Journal of the American Chemical Society, volume 138, issue 13, pages 4368-4376

Single Hydrogen Bond Donation from Flavin N5 to Proximal Asparagine Ensures FAD Reduction in DNA Photolyase

Wijaya I M Mahaputra
IWATA Tatsuya
Getzoff Elizabeth D. 2
Ito Shota
2
 
Department of Integrative Structural and Computational Biology and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, La Jolla, California 92037, United States
Publication typeJournal Article
Publication date2016-03-22
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor15
ISSN00027863, 15205126
General Chemistry
Catalysis
Biochemistry
Colloid and Surface Chemistry
Abstract
The spread of the absorbance of the stable FADH(•) radical (300-700 nm) allows CPD photolyase to highly efficiently form FADH(-), making it functional for DNA repair. In this study, FTIR spectroscopy detected a strong hydrogen bond, from FAD N5-H to the carbonyl group of the Asn378 side chain, that is modulated by the redox state of FAD. The observed characteristic frequency shifts were reproduced in quantum-mechanical models of the flavin binding site, which were then employed to elucidate redox tuning governed by Asn378. We demonstrate that enhanced hydrogen bonding of the Asn378 side chain with the FADH(•) radical increases thermodynamic stabilization of the radical state, and further ensures kinetic stabilization and accumulation of the fully reduced FADH(-) state.

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Wijaya I. M. M. et al. Single Hydrogen Bond Donation from Flavin N5 to Proximal Asparagine Ensures FAD Reduction in DNA Photolyase // Journal of the American Chemical Society. 2016. Vol. 138. No. 13. pp. 4368-4376.
GOST all authors (up to 50) Copy
Wijaya I. M. M., Domratcheva T., IWATA T., Getzoff E. D., Ito S. Single Hydrogen Bond Donation from Flavin N5 to Proximal Asparagine Ensures FAD Reduction in DNA Photolyase // Journal of the American Chemical Society. 2016. Vol. 138. No. 13. pp. 4368-4376.
RIS |
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RIS Copy
TY - JOUR
DO - 10.1021/jacs.5b10533
UR - https://doi.org/10.1021%2Fjacs.5b10533
TI - Single Hydrogen Bond Donation from Flavin N5 to Proximal Asparagine Ensures FAD Reduction in DNA Photolyase
T2 - Journal of the American Chemical Society
AU - Wijaya, I M Mahaputra
AU - Getzoff, Elizabeth D.
AU - Domratcheva, Tatiana
AU - IWATA, Tatsuya
AU - Ito, Shota
PY - 2016
DA - 2016/03/22 00:00:00
PB - American Chemical Society (ACS)
SP - 4368-4376
IS - 13
VL - 138
SN - 0002-7863
SN - 1520-5126
ER -
BibTex |
Cite this
BibTex Copy
@article{2016_Wijaya
author = {I M Mahaputra Wijaya and Elizabeth D. Getzoff and Tatiana Domratcheva and Tatsuya IWATA and Shota Ito},
title = {Single Hydrogen Bond Donation from Flavin N5 to Proximal Asparagine Ensures FAD Reduction in DNA Photolyase},
journal = {Journal of the American Chemical Society},
year = {2016},
volume = {138},
publisher = {American Chemical Society (ACS)},
month = {mar},
url = {https://doi.org/10.1021%2Fjacs.5b10533},
number = {13},
pages = {4368--4376},
doi = {10.1021/jacs.5b10533}
}
MLA
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MLA Copy
Wijaya, I. M. Mahaputra, et al. “Single Hydrogen Bond Donation from Flavin N5 to Proximal Asparagine Ensures FAD Reduction in DNA Photolyase.” Journal of the American Chemical Society, vol. 138, no. 13, Mar. 2016, pp. 4368-4376. https://doi.org/10.1021%2Fjacs.5b10533.
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