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Open access
International Journal of Molecular Sciences, volume 22, issue 19, pages 10221

Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection

Averina Olga A 1, 2
, 
Permyakov Oleg A 1
, 
Grigorieva Olga O 1
, 
Starshin Alexey S 3
, 
Mazur Alexander M 3
, 
Prokhortchouk Egor B 3
, 
Dontsova Olga A. 4, 5, 6
, 
Sergiev Petr V. 1, 4, 5
1
 
2
 
3
 
4
 
5
 
6
 
Publication type: Journal Article
Publication date: 2021-09-23
Quartile SCImago
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Impact factor: 5.6
ISSN: 16616596, 14220067
Catalysis
Organic Chemistry
Inorganic Chemistry
Physical and Theoretical Chemistry
Computer Science Applications
Spectroscopy
Molecular Biology
General Medicine
Abstract

Genome editing is an indispensable tool for functional genomics. The caveat of the genome-editing pipeline is a prevalence of error-prone non-homologous end joining over homologous recombination, while only the latter is suitable to introduce particularly desired genetic variants. To overcome this problem, a toolbox of genome engineering was appended by a variety of improved instruments. In this work, we compared the efficiency of a number of recently suggested improved systems for genome editing applied to the same genome regions on a murine zygote model via microinjection. As a result, we observed that homologous recombination utilizing an ssDNA template following sgRNA directed Cas9 cleavage is still the method of choice for the creation of animals with precise genome alterations.

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Averina O. A. et al. Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection // International Journal of Molecular Sciences. 2021. Vol. 22. No. 19. p. 10221.
GOST all authors (up to 50) Copy
Averina O. A., Permyakov O. A., Grigorieva O. O., Starshin A. S., Mazur A. M., Prokhortchouk E. B., Dontsova O. A., Sergiev P. V. Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection // International Journal of Molecular Sciences. 2021. Vol. 22. No. 19. p. 10221.
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TY - JOUR
DO - 10.3390/ijms221910221
UR - https://doi.org/10.3390%2Fijms221910221
TI - Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection
T2 - International Journal of Molecular Sciences
AU - Averina, Olga A
AU - Prokhortchouk, Egor B
AU - Dontsova, Olga A.
AU - Permyakov, Oleg A
AU - Mazur, Alexander M
AU - Grigorieva, Olga O
AU - Starshin, Alexey S
AU - Sergiev, Petr V.
PY - 2021
DA - 2021/09/23 00:00:00
PB - Multidisciplinary Digital Publishing Institute (MDPI)
SP - 10221
IS - 19
VL - 22
SN - 1661-6596
SN - 1422-0067
ER -
BibTex |
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BibTex Copy
@article{2021_Averina,
author = {Olga A Averina and Egor B Prokhortchouk and Olga A. Dontsova and Oleg A Permyakov and Alexander M Mazur and Olga O Grigorieva and Alexey S Starshin and Petr V. Sergiev},
title = {Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection},
journal = {International Journal of Molecular Sciences},
year = {2021},
volume = {22},
publisher = {Multidisciplinary Digital Publishing Institute (MDPI)},
month = {sep},
url = {https://doi.org/10.3390%2Fijms221910221},
number = {19},
pages = {10221},
doi = {10.3390/ijms221910221}
}
MLA
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Averina, Olga A., et al. “Comparative analysis of genome editors efficiency on a model of mice zygotes microinjection.” International Journal of Molecular Sciences, vol. 22, no. 19, Sep. 2021, p. 10221. https://doi.org/10.3390%2Fijms221910221.
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