The São Paulo School of Advanced Science in CryoEM

The São Paulo School of Advanced Science in CryoEM aims to cover the theoretical and practical foundations of advanced CryoEM techniques used to determine macromolecular structures. Time will be divided between lectures, demonstrations and hands-on practical sessions given by internationally renowned researchers in the field of CryoEM. Topics that will be covered include sample preparation, data collection, processing of high-resolution image data, single particle analysis, in-cell structural biology by cryo electron tomography (Cryo-TM), model building and model validation. Invited and participating researchers will also present their most recent results.

The School is being organized by researchers from the University of São Paulo and the National Center for Research in Energy and Materials (CNPEM) and supported by FAPESP.

The course is aimed at graduate students and post-docs. Undergraduate students and young PIs may also apply. Candidates are expected to be fluent in English. Students participating in the course will be given the opportunity to present their research projects in poster presentations.

Attendance will be limited to 70 students chosen by the Organizing Committee from applicants based in Brazil and from other countries. Those selected will receive transportation and daily allowances (except for students currently receiving a FAPESP scholarship).

A detailed program will be made available after participant selection. A preliminary list of topics can be found below:

• Historical perspective of single particle EM and CryoEM

• Electron microscope architecture

• Detectors and Motion Correction

• Sample Preparation and Vitrification

• Symmetry

• Fourier Transforms

• Cryo-EM data collection

• Contrast Transfer Function

• Masks and Filters

• 2D and 3D classification

• Conformational Heterogeneity

• Membrane proteins (sample preparation, data collection and processing)

• Cryo-electron tomography, subtomogram averaging and in-cell structural

• Atomic Model Building

• Helical reconstruction

• Validation of Models and Maps

• Data deposition and Databases

• Practical Sessions in Single Particle Analysis and subtomogram averaging

• Oral presentations from participating PIs illustrating the application of CryoEM and CryoET methods to investigate biological system

• Invited student oral presentations

• Poster sessions

Application Deadline: March 1, 2023.

Selected participants will be required to pay a registration fee of R$ 500 for South American participants or US$ 300 for participants from outside South America.

Registration includes air travel* and accommodation in a shared double room.

Students should bring their personal lap-tops for use in the practical sessions. Your laptops should have a port to connect an ethernet cable. If not, you should bring a separate ethernet cable adapter.

* Air travel will only be provided for participants living outside of state of São Paulo