Open Access
Down-regulation of RNA Helicase II/Gu Results in the Depletion of 18 and 28 S rRNAs in Xenopus Oocyte
Hushan Yang
1
,
Juhua Zhou
2
,
Robert L. Ochs
3
,
Dale HENNING
2
,
Runyan Jin
2
,
Benigno C. Valdez
2
3
Precision Therapeutics, Inc., Pittsburgh, Pennsylvania 15203
|
Publication type: Journal Article
Publication date: 2003-10-01
scimago Q1
wos Q2
SJR: 1.705
CiteScore: 7.6
Impact factor: 3.9
ISSN: 00219258, 1083351X
PubMed ID:
12851405
Biochemistry
Molecular Biology
Cell Biology
Abstract
Genetic manipulations have revealed the functions of RNA helicases in ribosomal RNA (rRNA) biogenesis in yeast. However, no report shows the role of an RNA helicase in rRNA formation in higher eukaryotes. This study reports the functional characterization of the frog homologue of nucleolar RNA helicase II/Gu (xGu or DDX21). Down-regulation of xGu in Xenopus laevis oocyte using an antisense oligodeoxynucleotide results in the depletion of 18 and 28 S rRNAs. The disappearance of 18 S rRNA is accompanied by an accumulation of 20 S, indicating that xGu is critical in the processing of 20 to 18 S rRNA. The degradation of 28 S rRNA into fragments smaller than 18 S is also associated with a specific decrease in the level of xGu protein. These effects are reversed in the presence of in vitro synthesized wild type xGu mRNA but not its helicase-deficient mutant form. Similar aberrant rRNA processing is observed when antibody against xGu is microinjected. The involvement of xGu in processing of rRNA is consistent with the localization of Gu protein to the granular and dense fibrillar components of PtK2 cell nucleoli by immunoelectron microscopy. Our results show that xGu is involved in the processing of 20 to 18 S rRNA and contributes to the stability of 28 S rRNA in Xenopus oocytes.
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Total citations:
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Citations from 2024:
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(8.34%)
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GOST
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Yang H. et al. Down-regulation of RNA Helicase II/Gu Results in the Depletion of 18 and 28 S rRNAs in Xenopus Oocyte // Journal of Biological Chemistry. 2003. Vol. 278. No. 40. pp. 38847-38859.
GOST all authors (up to 50)
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Yang H., Zhou J., Ochs R. L., HENNING D., Jin R., Valdez B. C. Down-regulation of RNA Helicase II/Gu Results in the Depletion of 18 and 28 S rRNAs in Xenopus Oocyte // Journal of Biological Chemistry. 2003. Vol. 278. No. 40. pp. 38847-38859.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1074/jbc.M302258200
UR - https://doi.org/10.1074/jbc.M302258200
TI - Down-regulation of RNA Helicase II/Gu Results in the Depletion of 18 and 28 S rRNAs in Xenopus Oocyte
T2 - Journal of Biological Chemistry
AU - Yang, Hushan
AU - Zhou, Juhua
AU - Ochs, Robert L.
AU - HENNING, Dale
AU - Jin, Runyan
AU - Valdez, Benigno C.
PY - 2003
DA - 2003/10/01
PB - American Society for Biochemistry and Molecular Biology
SP - 38847-38859
IS - 40
VL - 278
PMID - 12851405
SN - 0021-9258
SN - 1083-351X
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2003_Yang,
author = {Hushan Yang and Juhua Zhou and Robert L. Ochs and Dale HENNING and Runyan Jin and Benigno C. Valdez},
title = {Down-regulation of RNA Helicase II/Gu Results in the Depletion of 18 and 28 S rRNAs in Xenopus Oocyte},
journal = {Journal of Biological Chemistry},
year = {2003},
volume = {278},
publisher = {American Society for Biochemistry and Molecular Biology},
month = {oct},
url = {https://doi.org/10.1074/jbc.M302258200},
number = {40},
pages = {38847--38859},
doi = {10.1074/jbc.M302258200}
}
Cite this
MLA
Copy
Yang, Hushan, et al. “Down-regulation of RNA Helicase II/Gu Results in the Depletion of 18 and 28 S rRNAs in Xenopus Oocyte.” Journal of Biological Chemistry, vol. 278, no. 40, Oct. 2003, pp. 38847-38859. https://doi.org/10.1074/jbc.M302258200.