Open Access
Open access
volume 9 issue 12 pages 2611

Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure

Josef Vodička 1
Martin Pesta 2, 3
VLASTIMIL KULDA 4
Katerina Houfkova 2
Bohuslava Vankova 5
Jakub Sebek 1
Martin Skala 1
Jakub Fichtl 1
Kristyna Prochazkova 1
Ondrej Topolcan 6
Publication typeJournal Article
Publication date2020-12-04
scimago Q1
wos Q2
SJR1.670
CiteScore10.5
Impact factor5.2
ISSN20734409
PubMed ID:  33291819
General Medicine
Abstract

The aim of the study was to compare the prognostic significance of lymph node status of patients with lung cancer analyzed by three different methods: hematoxylin and eosin (H&E), immunohistochemistry of cytokeratin 19 (IHC CK19), and One-Step Nucleic Acid Amplification (OSNA). The clinical relevance of the results was evaluated based on relation to prognosis; the disease-free interval (DFI) and overall survival (OS) were analyzed. During radical surgical treatment, a total of 1426 lymph nodes were obtained from 100 patients, creating 472 groups of nodes (4–5 groups per patient) and examined by H&E, IHC CK19 and OSNA. The median follow-up was 44 months. Concordant results on the lymph node status of the H&E, IHC CK19 and OSNA examinations were reported in 78% of patients. We recorded shorter OS in patients with positive results provided by both OSNA and H&E. The study demonstrated a higher percentage of detected micrometastases in lymph nodes by the OSNA method. However, the higher sensitivity of the OSNA, with the cut-off value 250 copies of mRNA of CK19/µL, resulted in a lower association of OSNA positivity with progress of the disease compared to H&E. Increasing the cut-off to 615 copies resulted in an increase in concordance between the OSNA and H&E, which means that the higher cut-off is more relevant in the case of lung tumors.

Found 
Found 

Top-30

Journals

1
Cells
1 publication, 20%
Biomedicine and Pharmacotherapy
1 publication, 20%
Medicina
1 publication, 20%
Cancers
1 publication, 20%
International Journal of Radiation Research
1 publication, 20%
1

Publishers

1
2
3
MDPI
3 publications, 60%
Elsevier
1 publication, 20%
Apex Publishing
1 publication, 20%
1
2
3
  • We do not take into account publications without a DOI.
  • Statistics recalculated weekly.

Are you a researcher?

Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
5
Share
Cite this
GOST |
Cite this
GOST Copy
Vodička J. et al. Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure // Cells. 2020. Vol. 9. No. 12. p. 2611.
GOST all authors (up to 50) Copy
Vodička J., Pesta M., KULDA V., Houfkova K., Vankova B., Sebek J., Skala M., Fichtl J., Prochazkova K., Topolcan O. Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure // Cells. 2020. Vol. 9. No. 12. p. 2611.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.3390/cells9122611
UR - https://doi.org/10.3390/cells9122611
TI - Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure
T2 - Cells
AU - Vodička, Josef
AU - Pesta, Martin
AU - KULDA, VLASTIMIL
AU - Houfkova, Katerina
AU - Vankova, Bohuslava
AU - Sebek, Jakub
AU - Skala, Martin
AU - Fichtl, Jakub
AU - Prochazkova, Kristyna
AU - Topolcan, Ondrej
PY - 2020
DA - 2020/12/04
PB - MDPI
SP - 2611
IS - 12
VL - 9
PMID - 33291819
SN - 2073-4409
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2020_Vodička,
author = {Josef Vodička and Martin Pesta and VLASTIMIL KULDA and Katerina Houfkova and Bohuslava Vankova and Jakub Sebek and Martin Skala and Jakub Fichtl and Kristyna Prochazkova and Ondrej Topolcan},
title = {Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure},
journal = {Cells},
year = {2020},
volume = {9},
publisher = {MDPI},
month = {dec},
url = {https://doi.org/10.3390/cells9122611},
number = {12},
pages = {2611},
doi = {10.3390/cells9122611}
}
MLA
Cite this
MLA Copy
Vodička, Josef, et al. “Prognostic Significance of Lymph Node Examination by the OSNA Method in Lung Cancer Patients—Comparison with the Standard Histopathological Procedure.” Cells, vol. 9, no. 12, Dec. 2020, p. 2611. https://doi.org/10.3390/cells9122611.