Purification and characterization of 40-kDa sterigmatocystinO-methyltransferase involved in aflatoxin biosynthesis
Publication type: Journal Article
Publication date: 1999-03-01
Toxicology
Abstract
Sterigmatocystin-O-methyltransferase (ST-OMTase), an enzyme catalyzing O-methylation of sterigmatocystin with S-adenosylmethionine (SAM), was purified to electrophoretic homogeneity by immunoaffinity chromatography. A novel spectrofluorometric method was established to quantitatively determine the enzymatic activity of ST-OMTase. The purified protein, with a molecular weight of 40 kDa by SDS-PAGE, was sensitive to thiol reagents and low concentrations of heavy metal ions. Using a nutritional shift assay, the expression patterns for ST-OMTase and the transcripts of its corresponding gene, omtA, correlated well with that for aflatoxin B(1) formation. Neither methyltransferase activity nor omtA, mRNA was detected in the fungal cultures of nonaflatoxigenic isolates, including A. flavus, A. sojae, A. nidulans and A. versicolor under optimal growing conditions for aflatoxin B(1) production.
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Liu B., Bhatnagar D., Chu F. S. Purification and characterization of 40-kDa sterigmatocystinO-methyltransferase involved in aflatoxin biosynthesis // Natural Toxins. 1999. Vol. 7. No. 2. pp. 63-69.
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Liu B., Bhatnagar D., Chu F. S. Purification and characterization of 40-kDa sterigmatocystinO-methyltransferase involved in aflatoxin biosynthesis // Natural Toxins. 1999. Vol. 7. No. 2. pp. 63-69.
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TY - JOUR
DO - 10.1002/(sici)1522-7189(199903/04)7:2<63::aid-nt41>3.0.co;2-t
UR - https://doi.org/10.1002/(sici)1522-7189(199903/04)7:2<63::aid-nt41>3.0.co;2-t
TI - Purification and characterization of 40-kDa sterigmatocystinO-methyltransferase involved in aflatoxin biosynthesis
T2 - Natural Toxins
AU - Liu, Biing-Hui
AU - Bhatnagar, Deepak
AU - Chu, Fun Sun
PY - 1999
DA - 1999/03/01
PB - Wiley
SP - 63-69
IS - 2
VL - 7
PMID - 10495468
SN - 1056-9014
SN - 1522-7189
ER -
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@article{1999_Liu,
author = {Biing-Hui Liu and Deepak Bhatnagar and Fun Sun Chu},
title = {Purification and characterization of 40-kDa sterigmatocystinO-methyltransferase involved in aflatoxin biosynthesis},
journal = {Natural Toxins},
year = {1999},
volume = {7},
publisher = {Wiley},
month = {mar},
url = {https://doi.org/10.1002/(sici)1522-7189(199903/04)7:2<63::aid-nt41>3.0.co;2-t},
number = {2},
pages = {63--69},
doi = {10.1002/(sici)1522-7189(199903/04)7:2<63::aid-nt41>3.0.co;2-t}
}
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Liu, Biing-Hui, et al. “Purification and characterization of 40-kDa sterigmatocystinO-methyltransferase involved in aflatoxin biosynthesis.” Natural Toxins, vol. 7, no. 2, Mar. 1999, pp. 63-69. https://doi.org/10.1002/(sici)1522-7189(199903/04)7:2<63::aid-nt41>3.0.co;2-t.