Current Protocols, volume 1, issue 1

Growth and Maintenance of Escherichia coli Laboratory Strains

Amie R Tuttle ¹

Nicholas D Trahan ¹

Mike S. Son ¹

Show full list: 3 authors

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Plymouth State University Plymouth New Hampshire |

Publication type: Journal Article

Publication date: 2021-01-20

Wiley

Journal: Current Protocols

scimago Q1

SJR: 1.520

CiteScore: 4.0

Impact factor: —

ISSN: 26911299

DOI: 10.1002/cpz1.20

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PubMed ID: 33484484

General Biochemistry, Genetics and Molecular Biology

General Immunology and Microbiology

General Pharmacology, Toxicology and Pharmaceutics

General Neuroscience

Medical Laboratory Technology

Health Informatics

Abstract

Escherichia coli is a Gram-negative bacterium, commonly used in both teaching and research laboratories. This article includes protocols for the growth and maintenance of E. coli in any teaching- or research-associated laboratory. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Growth of E. coli from frozen stocks Basic Protocol 2: Growth of E. coli in liquid media Basic Protocol 3: Enumeration of E. coli on solid media Basic Protocol 4: Storage of E. coli frozen stocks in glycerol Basic Protocol 5: Storage of E. coli in agar stabs Basic Protocol 6: Growth curve of E. coli liquid culture

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Metrics

CITATIONS

68 Total citations

62 Recent citations

11 Field Citation Ratio

2.73 Relative Citation Ratio

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GOST |

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GOST Copy

Tuttle A. R., Trahan N. D., Son M. S. Growth and Maintenance of Escherichia coli Laboratory Strains // Current Protocols. 2021. Vol. 1. No. 1.

GOST all authors (up to 50) Copy

Tuttle A. R., Trahan N. D., Son M. S. Growth and Maintenance of Escherichia coli Laboratory Strains // Current Protocols. 2021. Vol. 1. No. 1.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1002/cpz1.20

UR - https://doi.org/10.1002/cpz1.20

TI - Growth and Maintenance of Escherichia coli Laboratory Strains

T2 - Current Protocols

AU - Tuttle, Amie R

AU - Trahan, Nicholas D

AU - Son, Mike S.

PY - 2021

DA - 2021/01/20

PB - Wiley

IS - 1

VL - 1

PMID - 33484484

SN - 2691-1299

ER -

BibTex

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BibTex (up to 50 authors) Copy

@article{2021_Tuttle,

author = {Amie R Tuttle and Nicholas D Trahan and Mike S. Son},

title = {Growth and Maintenance of Escherichia coli Laboratory Strains},

journal = {Current Protocols},

year = {2021},

volume = {1},

publisher = {Wiley},

month = {jan},

url = {https://doi.org/10.1002/cpz1.20},

number = {1},

doi = {10.1002/cpz1.20}

}

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Publisher

Wiley

Journal

Current Protocols

scimago Q1

SJR

1.520

CiteScore

4.0

Impact factor

—

ISSN

26911299 (Print, Electronic)