Cytometry. Part A : the journal of the International Society for Analytical Cytology

, volume 89 , issue 11 , pages 1010-1016

Fluorescence‐free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets

A L Litvinenko ^{1, 2}

Alexander E. Moskalensky ^{1, 2}

N A Karmadonova ³

Vyacheslav M. Nekrasov ^{1, 2}

D I Strokotov ^{1, 4}

A I Konokhova ¹

Maxim A. Yurkin ^{1, 2}

E A Pokushalov ³

A. V. Chernyshev ^{1, 2}

V.P. Mal'tsev ^{1, 2, 4}

Hide authors affiliations Show authors affiliations: 4 affiliations

Voevodsky Institute of Chemical Kinetics and Combustion; Novosibirsk Russian Federation |

NOVOSIBIRSK STATE UNIVERSITY; NOVOSIBIRSK RUSSIAN FEDERATION |

State Research Institute of Circulation Pathology; Novosibirsk Russian Federation |

⁴

Novosibirsk State Medical University; Novosibirsk Russian Federation |

Publication type: Journal Article

Publication date: 2016-10-21

Wiley

Cytometry. Part A : the journal of the International Society for Analytical Cytology

scimago Q1

wos Q3

SJR: 0.942

CiteScore: 6.2

Impact factor: 2.1

ISSN: 15524922, 15524930

DOI: 10.1002/cyto.a.23003

Copy DOI

PubMed ID: 27768824

Cell Biology

Histology

Pathology and Forensic Medicine

Abstract

Whereas commercially available hematological analyzers measure volume of individual platelets, angle‐resolved light‐scattering provides unique ability to additionally measure their shape index. We utilized the scanning flow cytometer to measure light‐scattering profiles (LSPs) of individual platelets taken from 16 healthy donors and the solution of the inverse light‐scattering problem to retrieve the volume and shape index of each platelet. In normal conditions, the platelet shape index distribution (PSID) demonstrates three peaks, which relate to resting, partially activated, and fully activated platelets. We developed an algorithm, based on fitting PSID by a sum of three peak functions, to determine the percentage, mean platelet shape index, and distribution width of each platelet fraction. In total, this method gives eight additional parameters of platelet morphology and function to be used in clinical hematological analysis. We also stimulated the platelets with adenosine diphosphate (ADP) and measured the dependence of equilibrium PSID, including the total percentage of activated platelets, on ADP concentration. © 2016 International Society for Advancement of Cytometry

Found

6 citations

Moskalensky Alexander

🥼 🤝

PhD in Physics and Mathematics

51 publications, 486 citations, 16 reviews

h-index: 13

Novosibirsk State University

Research interests

"Smart" materials

Biomedical instrumentation

Biophotonics

Biophysics

Luminescence

Photodynamic Therapy (PDT)

Targeted drug delivery

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Litvinenko A. L. et al. Fluorescence‐free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets // Cytometry. Part A : the journal of the International Society for Analytical Cytology. 2016. Vol. 89. No. 11. pp. 1010-1016.

GOST all authors (up to 50) Copy

Litvinenko A. L., Moskalensky A. E., Karmadonova N. A., Nekrasov V. M., Strokotov D. I., Konokhova A. I., Yurkin M. A., Pokushalov E. A., Chernyshev A. V., Mal'tsev V. Fluorescence‐free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets // Cytometry. Part A : the journal of the International Society for Analytical Cytology. 2016. Vol. 89. No. 11. pp. 1010-1016.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1002/cyto.a.23003

UR - https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.23003

TI - Fluorescence‐free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets

T2 - Cytometry. Part A : the journal of the International Society for Analytical Cytology

AU - Litvinenko, A L

AU - Moskalensky, Alexander E.

AU - Karmadonova, N A

AU - Nekrasov, Vyacheslav M.

AU - Strokotov, D I

AU - Konokhova, A I

AU - Yurkin, Maxim A.

AU - Pokushalov, E A

AU - Chernyshev, A. V.

AU - Mal'tsev, V.P.

PY - 2016

DA - 2016/10/21

PB - Wiley

SP - 1010-1016

IS - 11

VL - 89

PMID - 27768824

SN - 1552-4922

SN - 1552-4930

ER -

BibTex |

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BibTex (up to 50 authors) Copy

@article{2016_Litvinenko,

author = {A L Litvinenko and Alexander E. Moskalensky and N A Karmadonova and Vyacheslav M. Nekrasov and D I Strokotov and A I Konokhova and Maxim A. Yurkin and E A Pokushalov and A. V. Chernyshev and V.P. Mal'tsev},

title = {Fluorescence‐free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets},

journal = {Cytometry. Part A : the journal of the International Society for Analytical Cytology},

year = {2016},

volume = {89},

publisher = {Wiley},

month = {oct},

url = {https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.23003},

number = {11},

pages = {1010--1016},

doi = {10.1002/cyto.a.23003}

}

MLA

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MLA Copy

Litvinenko, A. L., et al. “Fluorescence‐free flow cytometry for measurement of shape index distribution of resting, partially activated, and fully activated platelets.” Cytometry. Part A : the journal of the International Society for Analytical Cytology, vol. 89, no. 11, Oct. 2016, pp. 1010-1016. https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.23003.