((E)‐N′(3,5‐di‐tert‐butil‐2‐hedroxybenzilidene)‐2‐hydroxybenzohydrazide (H3sahz) ₂ Copper (II) Complex: Synthesis, Crystal Structures, in silico Evaluations, and Enzymatic Inhibition

In this study, (E)‐N′(3,5‐di‐tert‐butil‐2‐hedroxybenzilidene)‐2‐hydroxybenzohydrazide (H₃sahz)₂ and its copper (II) complex has been synthesized and evaluated by methods FTIR, UV–Vis, EPR, and single crystal X‐ray analysis. It has been shown, that H₃sahz crystallizes as a dimer through hydrogen bonds. H₃sahz with copper nitrate forms [Cu(H₂sahz)(NO₃)(H₂O)] complex, which according to X‐ray diffraction analysis has a distorted square pyramidal structure. The complex was screened for α‐glucosidase (α‐Glu), acetylcholinesterase (AChE), and butyrylcholinesterase (BChE) inhibitory abilities. Results displayed that IC₅₀ and K_i values of the novel complex for AChE, BChE, and α‐Glu enzymes were obtained at 0.93–2.14, 1.01–2.03, and 73.86–102.65 μM, respectively. The molecular docking outcomes have shown that the synthetic complex has a lower affinity for α‐glucosidase compared to acarbose. But the inhibition ability of H₃sahz for acetylcholinesterase and butyrylcholinesterase enzymes was greater than that of tacrine. These findings indicate that the (H₃sahz)₂ complex may be considered a possible candidate for the development and discovery of compounds effective in inhibiting the relevant enzymes.