Biomedical Chromatography, volume 32, issue 7, pages e4216
Determination of homogentisic acid in urine for diagnosis of alcaptonuria: Capillary electrophoretic method optimization using experimental design
Publication type: Journal Article
Publication date: 2018-03-30
Journal:
Biomedical Chromatography
Quartile SCImago
Q3
Quartile WOS
Q3
Impact factor: 1.8
ISSN: 02693879, 10990801
DOI:
10.1002/bmc.4216
Drug Discovery
Biochemistry
Molecular Biology
General Medicine
Pharmacology
Clinical Biochemistry
Analytical Chemistry
Abstract
Homogentisic acid (HGA) is a diagnostic metabolite that accumulates in the urine and tissues of patients with alkaptonuria which is a rare autosomal recessive disease. HGA is a specific metabolite in urine and serum, which is used for diagnosis of alkaptonuria. This study presents an inexpensive and easy capillary electrophoretic method for the quantitative determination of HGA in urine samples. The method was optimized using full factorial experimental design. The optimal separation electrolyte and separation voltage were revealed as 45 mmol/L phosphate buffer at pH 7.0 and 22 kV, respectively. Under these conditions the presence of HGA was detected in 6 min. Repeatability of migration times and corrected peak areas of HGA (as RSD) were 0.37 and 1.99, respectively. The detection limit was 0.56 μg/mL, 3 times of the average noise, and the quantification limit was 1.85 μg/mL, 10 times the average noise for HGA. Urine samples were directly injected to the capillary without any pretreatment step.
Top-30
Citations by journals
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Annals of Clinical Biochemistry
1 publication, 16.67%
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Journal of Separation Science
1 publication, 16.67%
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Journal of Pharmaceutical and Biomedical Analysis
1 publication, 16.67%
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Bioelectrochemistry
1 publication, 16.67%
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Microchemical Journal
1 publication, 16.67%
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Russian Chemical Reviews
1 publication, 16.67%
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Citations by publishers
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Elsevier
3 publications, 50%
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SAGE
1 publication, 16.67%
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Wiley
1 publication, 16.67%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 16.67%
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- We do not take into account publications without a DOI.
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Öztekin N., Balta G. S., Cansever M. Ş. Determination of homogentisic acid in urine for diagnosis of alcaptonuria: Capillary electrophoretic method optimization using experimental design // Biomedical Chromatography. 2018. Vol. 32. No. 7. p. e4216.
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Öztekin N., Balta G. S., Cansever M. Ş. Determination of homogentisic acid in urine for diagnosis of alcaptonuria: Capillary electrophoretic method optimization using experimental design // Biomedical Chromatography. 2018. Vol. 32. No. 7. p. e4216.
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TY - JOUR
DO - 10.1002/bmc.4216
UR - https://doi.org/10.1002/bmc.4216
TI - Determination of homogentisic acid in urine for diagnosis of alcaptonuria: Capillary electrophoretic method optimization using experimental design
T2 - Biomedical Chromatography
AU - Balta, Gülfem S
AU - Cansever, M Şerif
AU - Öztekin, Nevin
PY - 2018
DA - 2018/03/30 00:00:00
PB - Wiley
SP - e4216
IS - 7
VL - 32
SN - 0269-3879
SN - 1099-0801
ER -
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@article{2018_Öztekin,
author = {Gülfem S Balta and M Şerif Cansever and Nevin Öztekin},
title = {Determination of homogentisic acid in urine for diagnosis of alcaptonuria: Capillary electrophoretic method optimization using experimental design},
journal = {Biomedical Chromatography},
year = {2018},
volume = {32},
publisher = {Wiley},
month = {mar},
url = {https://doi.org/10.1002/bmc.4216},
number = {7},
pages = {e4216},
doi = {10.1002/bmc.4216}
}
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Öztekin, Nevin, et al. “Determination of homogentisic acid in urine for diagnosis of alcaptonuria: Capillary electrophoretic method optimization using experimental design.” Biomedical Chromatography, vol. 32, no. 7, Mar. 2018, p. e4216. https://doi.org/10.1002/bmc.4216.