Biomedical Chromatography, volume 35, issue 2
Metabolic profiling of aromatic compounds in cerebrospinal fluid of neurosurgical patients using microextraction by packed sorbent and liquid–liquid extraction with gas chromatography–mass spectrometry analysis
Alisa K. Pautova
1
,
Zoya B Khesina
2
,
Tatiana N. Litvinova
1
,
Alexander I Revelsky
3
,
Natalia V. Beloborodova
1
Publication type: Journal Article
Publication date: 2020-09-09
Journal:
Biomedical Chromatography
Quartile SCImago
Q3
Quartile WOS
Q3
Impact factor: 1.8
ISSN: 02693879, 10990801
DOI:
10.1002/bmc.4969
Drug Discovery
Biochemistry
Molecular Biology
General Medicine
Pharmacology
Clinical Biochemistry
Analytical Chemistry
Abstract
A new approach to the quantitative analysis of aromatic metabolites in cerebrospinal fluid samples of neurosurgical patients based on microextraction by packed sorbent coupled with derivatization and GC-MS was developed. Analytical characteristics such as recoveries (40-90%), limit of detection (0.1-0.3 μm) and limit of quantitation (0.4-0.7 μm) values, accuracy (<±20%), precision (<20%) and linear correlations (R2 ≥ 0.99) over a 0.4-10 μm range of concentrations demonstrated that microextraction by packed sorbent provides results for the quantitative analysis of target compounds comparable with those for liquid-liquid extraction. Similar results were achieved using 40 μl of sample for microextraction by packed sorbent instead of 200 μl for liquid-liquid extraction. Benzoic, 3-phenylpropionic, 3-phenyllactic, 4-hydroxybenzoic, 2-(4-hydroxyphenyl)acetic, homovanillic and 3-(4-hydroxyphenyl)lactic acids were found in cerebrospinal fluid samples (n = 138) of neurosurgical patients in lower concentrations than in serum samples (n = 110) of critically ill patients. Analysis of the cerebrospinal fluid and serum samples taken at the same time from neurosurgical patients (n = 5) revealed similar results for patients without infection and multidirectional results for patients with central nervous system infection. Our preliminary results demonstrate the necessity of further evaluating the aromatic compound profile in cerebrospinal fluid for its subsequent verification for potential diagnostic markers.
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Pautova A. K. et al. Metabolic profiling of aromatic compounds in cerebrospinal fluid of neurosurgical patients using microextraction by packed sorbent and liquid–liquid extraction with gas chromatography–mass spectrometry analysis // Biomedical Chromatography. 2020. Vol. 35. No. 2.
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Pautova A. K., Khesina Z. B., Litvinova T. N., Revelsky A. I., Beloborodova N. V. Metabolic profiling of aromatic compounds in cerebrospinal fluid of neurosurgical patients using microextraction by packed sorbent and liquid–liquid extraction with gas chromatography–mass spectrometry analysis // Biomedical Chromatography. 2020. Vol. 35. No. 2.
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TY - JOUR
DO - 10.1002/bmc.4969
UR - https://doi.org/10.1002/bmc.4969
TI - Metabolic profiling of aromatic compounds in cerebrospinal fluid of neurosurgical patients using microextraction by packed sorbent and liquid–liquid extraction with gas chromatography–mass spectrometry analysis
T2 - Biomedical Chromatography
AU - Khesina, Zoya B
AU - Litvinova, Tatiana N.
AU - Revelsky, Alexander I
AU - Pautova, Alisa K.
AU - Beloborodova, Natalia V.
PY - 2020
DA - 2020/09/09 00:00:00
PB - Wiley
IS - 2
VL - 35
SN - 0269-3879
SN - 1099-0801
ER -
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@article{2020_Pautova,
author = {Zoya B Khesina and Tatiana N. Litvinova and Alexander I Revelsky and Alisa K. Pautova and Natalia V. Beloborodova},
title = {Metabolic profiling of aromatic compounds in cerebrospinal fluid of neurosurgical patients using microextraction by packed sorbent and liquid–liquid extraction with gas chromatography–mass spectrometry analysis},
journal = {Biomedical Chromatography},
year = {2020},
volume = {35},
publisher = {Wiley},
month = {sep},
url = {https://doi.org/10.1002/bmc.4969},
number = {2},
doi = {10.1002/bmc.4969}
}