Biomedical Chromatography, volume 35, issue 2
Rapid simultaneous determination of gut microbial phenylalanine, tyrosine, and tryptophan metabolites in rat serum, urine, and faeces using LC–MS/MS and its application to a type 2 diabetes mellitus study
Bao Rong Zou
1
,
Yangwen Sun
1
,
Zengmei Xu
1
,
Yongda Chen
2
,
Lin Li
2
,
Lin Lei
1
,
Shaobao Zhang
1
,
Qiongfeng Liao
2
,
Zhiyong Xie
1, 3
3
Guangdong Provincial Key Laboratory of New Drug Design and Evaluation Guangzhou China
|
Publication type: Journal Article
Publication date: 2020-11-17
Journal:
Biomedical Chromatography
Quartile SCImago
Q3
Quartile WOS
Q3
Impact factor: 1.8
ISSN: 02693879, 10990801
DOI:
10.1002/bmc.4985
Drug Discovery
Biochemistry
Molecular Biology
General Medicine
Pharmacology
Clinical Biochemistry
Analytical Chemistry
Abstract
Gut microbial phenylalanine, tyrosine, and tryptophan metabolites are closely linked to various diseases. Monitoring the alterations of the related metabolites is vital to facilitate the understanding of pathophysiology of diseases. Herein, a rapid and sensitive assay based on LC-tandem mass spectrometry has been developed to analyze 20 gut microbial metabolites derived from phenylalanine, tyrosine, and tryptophan in rat serum, urine, and faeces. These microbial-derived metabolites were separated on a phenyl-hexyl column and simultaneously determined in a single run of 8 min. The detection limit for analytes ranged between 1.08 and 32.4 ng/mL. All calibration curves exhibited good linear relationships (R2 ≥ 0.9982). Intra- and inter-assay precision values were below 15% and accuracies ranged from 85% to 115% for all analytes. The selectivity, matrix effect, and recovery of this method were all satisfactory. The validated method was successfully applied to characterize the alterations of these metabolites in type 2 diabetes mellitus rat. In general, the developed assay is suitable for high-throughput monitoring of gut microbial phenylalanine, tyrosine, and tryptophan metabolites and provides a useful approach for exploring the mechanisms of microbial-derived metabolites in diseases.
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- We do not take into account publications without a DOI.
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Zou B. R. et al. Rapid simultaneous determination of gut microbial phenylalanine, tyrosine, and tryptophan metabolites in rat serum, urine, and faeces using LC–MS/MS and its application to a type 2 diabetes mellitus study // Biomedical Chromatography. 2020. Vol. 35. No. 2.
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Zou B. R., Sun Y., Xu Z., Chen Y., Li L., Lei L., Zhang S., Liao Q., Xie Z. Rapid simultaneous determination of gut microbial phenylalanine, tyrosine, and tryptophan metabolites in rat serum, urine, and faeces using LC–MS/MS and its application to a type 2 diabetes mellitus study // Biomedical Chromatography. 2020. Vol. 35. No. 2.
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TY - JOUR
DO - 10.1002/bmc.4985
UR - https://doi.org/10.1002/bmc.4985
TI - Rapid simultaneous determination of gut microbial phenylalanine, tyrosine, and tryptophan metabolites in rat serum, urine, and faeces using LC–MS/MS and its application to a type 2 diabetes mellitus study
T2 - Biomedical Chromatography
AU - Sun, Yangwen
AU - Xu, Zengmei
AU - Chen, Yongda
AU - Lei, Lin
AU - Zhang, Shaobao
AU - Zou, Bao Rong
AU - Liao, Qiongfeng
AU - Xie, Zhiyong
AU - Li, Lin
PY - 2020
DA - 2020/11/17 00:00:00
PB - Wiley
IS - 2
VL - 35
SN - 0269-3879
SN - 1099-0801
ER -
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@article{2020_Zou,
author = {Yangwen Sun and Zengmei Xu and Yongda Chen and Lin Lei and Shaobao Zhang and Bao Rong Zou and Qiongfeng Liao and Zhiyong Xie and Lin Li},
title = {Rapid simultaneous determination of gut microbial phenylalanine, tyrosine, and tryptophan metabolites in rat serum, urine, and faeces using LC–MS/MS and its application to a type 2 diabetes mellitus study},
journal = {Biomedical Chromatography},
year = {2020},
volume = {35},
publisher = {Wiley},
month = {nov},
url = {https://doi.org/10.1002/bmc.4985},
number = {2},
doi = {10.1002/bmc.4985}
}