ChemBioChem, volume 23, issue 15

Chemical Modifications for a Next Generation of Nucleic Acid Aptamers

Kwing Yeung Chan ¹

Andrew Brian Kinghorn ¹

Marcel Hollenstein ²

Julian Tanner ¹

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School of Biomedical Sciences LKS Faculty of Medicine The University of Hong Kong 21 Sassoon Road, Pokfulam Hong Kong SAR P. R. China |

Laboratory for Bioorganic Chemistry of Nucleic Acids Department of Structural Biology and Chemistry Institut Pasteur, CNRS UMR-3523 75015 Paris France |

Publication type: Journal Article

Publication date: 2022-04-29

Wiley

Journal: ChemBioChem

Quartile SCImago

Quartile WOS

Impact factor: 3.2

ISSN: 14394227, 14397633

DOI: 10.1002/cbic.202200006

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Organic Chemistry

Biochemistry

Molecular Biology

Molecular Medicine

Abstract

In the past three decades, in vitro systematic evolution of ligands by exponential enrichment (SELEX) has yielded many aptamers for translational applications in both research and clinical settings. Despite their promise as an alternative to antibodies, the low success rate of SELEX (∼30 %) has been a major bottleneck that hampers the further development of aptamers. One hurdle is the lack of chemical diversity in nucleic acids. To address this, the aptamer chemical repertoire has been extended by introducing exotic chemical groups, which provide novel binding functionalities. This review will focus on how modified aptamers can be selected and evolved, with illustration of some successful examples. In particular, unique chemistries are exemplified. Various strategies of incorporating modified building blocks into the standard SELEX protocol are highlighted, with a comparison of the differences between pre-SELEX and post-SELEX modifications. Nucleic acid aptamers with extended functionality evolved from non-natural chemistries will open up new vistas for function and application of nucleic acids.

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Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 6, 23.08% Multidisciplinary Digital Publishing Institute (MDPI) 6 publications, 23.08%
American Chemical Society (ACS)	American Chemical Society (ACS), 5, 19.23% American Chemical Society (ACS) 5 publications, 19.23%
Elsevier	Elsevier, 5, 19.23% Elsevier 5 publications, 19.23%
Wiley	Wiley, 4, 15.38% Wiley 4 publications, 15.38%
Springer Nature	Springer Nature, 2, 7.69% Springer Nature 2 publications, 7.69%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 1, 3.85% Royal Society of Chemistry (RSC) 1 publication, 3.85%
Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii	Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii, 1, 3.85% Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii 1 publication, 3.85%
The University of Jordan	The University of Jordan, 1, 3.85% The University of Jordan 1 publication, 3.85%
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Chan K. Y. et al. Chemical Modifications for a Next Generation of Nucleic Acid Aptamers // ChemBioChem. 2022. Vol. 23. No. 15.

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Chan K. Y., Kinghorn A. B., Hollenstein M., Tanner J. Chemical Modifications for a Next Generation of Nucleic Acid Aptamers // ChemBioChem. 2022. Vol. 23. No. 15.

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RIS Copy

TY - JOUR

DO - 10.1002/cbic.202200006

UR - https://doi.org/10.1002/cbic.202200006

TI - Chemical Modifications for a Next Generation of Nucleic Acid Aptamers

T2 - ChemBioChem

AU - Chan, Kwing Yeung

AU - Kinghorn, Andrew Brian

AU - Hollenstein, Marcel

AU - Tanner, Julian

PY - 2022

DA - 2022/04/29 00:00:00

PB - Wiley

IS - 15

VL - 23

SN - 1439-4227

SN - 1439-7633

ER -

BibTex

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@article{2022_Chan,

author = {Kwing Yeung Chan and Andrew Brian Kinghorn and Marcel Hollenstein and Julian Tanner},

title = {Chemical Modifications for a Next Generation of Nucleic Acid Aptamers},

journal = {ChemBioChem},

year = {2022},

volume = {23},

publisher = {Wiley},

month = {apr},

url = {https://doi.org/10.1002/cbic.202200006},

number = {15},

doi = {10.1002/cbic.202200006}

}

Found error?

Publisher

Wiley

Journal

ChemBioChem

Quartile SCImago

Quartile WOS

Impact factor

3.2

ISSN

14394227 (Print)
14397633 (Electronic)