Temperature-Mediated Regulation of Enzymatic Activity

Controllable nanobiocatalysis using enzymes has evolved to play an important role in the specific and efficient preparation of bioproducts. Temperature change is one promising tool owing to the dependence of enzyme catalysis on temperature. Some examples of regulation of enzymatic activity through direct or indirect heating methods with the assistance of thermoresponsive or thermal harvesting materials are summarized in this concept article. Several fascinating applications including remote control of enzyme and/or substrate release, on-off enzyme reactions triggered by temperature variation to separate products from enzymes and remote acceleration of enzyme reactions even at room temperature have been highlighted. All this provides a new perspective for establishing on-demand enzyme catalysis to further their applications in biological engineering, chemical industry, and scientific research fields.