Synthesis of 3′‐BODIPY‐Labeled Active Esters of Nucleotides and a Chemical Primer Extension Assay on Beads
Kerstin Gießler
1
,
Helmut Griesser
2
,
Daniela Göhringer
2
,
Thomas Sabirov
2
,
Clemens Richert
3, 4
Publication type: Journal Article
Publication date: 2010-05-21
scimago Q2
wos Q2
SJR: 0.558
CiteScore: 4.3
Impact factor: 2.7
ISSN: 1434193X, 10990690
Organic Chemistry
Physical and Theoretical Chemistry
Abstract
A solution-phase synthesis of active esters of 3′-fluorophore-labeled deoxynucleoside 5′-monophosphates was developed for thymine and cytosine as nucleobases by using two different BODIPY dyes. Starting from the respective 2′-amino-2′,3′-dideoxynucleoside-5′-monophosphate, the fluorescent oxyazabenzotriazolides can be prepared in one-pot procedures involving labeling and activation. Screening of a range of supports led to a chemical primer extension assay on beads with in situ detection of nucleobases in target DNA through optical read-out.
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Metrics
49
Total citations:
49
Citations from 2024:
5
(10.2%)
The most citing journal
Citations in journal:
2
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MLA
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GOST
Copy
Gießler K. et al. Synthesis of 3′‐BODIPY‐Labeled Active Esters of Nucleotides and a Chemical Primer Extension Assay on Beads // European Journal of Organic Chemistry. 2010. Vol. 2010. No. 19. pp. 3611-3620.
GOST all authors (up to 50)
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Gießler K., Griesser H., Göhringer D., Sabirov T., Richert C. Synthesis of 3′‐BODIPY‐Labeled Active Esters of Nucleotides and a Chemical Primer Extension Assay on Beads // European Journal of Organic Chemistry. 2010. Vol. 2010. No. 19. pp. 3611-3620.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1002/ejoc.201000210
UR - https://doi.org/10.1002/ejoc.201000210
TI - Synthesis of 3′‐BODIPY‐Labeled Active Esters of Nucleotides and a Chemical Primer Extension Assay on Beads
T2 - European Journal of Organic Chemistry
AU - Gießler, Kerstin
AU - Griesser, Helmut
AU - Göhringer, Daniela
AU - Sabirov, Thomas
AU - Richert, Clemens
PY - 2010
DA - 2010/05/21
PB - Wiley
SP - 3611-3620
IS - 19
VL - 2010
SN - 1434-193X
SN - 1099-0690
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2010_Gießler,
author = {Kerstin Gießler and Helmut Griesser and Daniela Göhringer and Thomas Sabirov and Clemens Richert},
title = {Synthesis of 3′‐BODIPY‐Labeled Active Esters of Nucleotides and a Chemical Primer Extension Assay on Beads},
journal = {European Journal of Organic Chemistry},
year = {2010},
volume = {2010},
publisher = {Wiley},
month = {may},
url = {https://doi.org/10.1002/ejoc.201000210},
number = {19},
pages = {3611--3620},
doi = {10.1002/ejoc.201000210}
}
Cite this
MLA
Copy
Gießler, Kerstin, et al. “Synthesis of 3′‐BODIPY‐Labeled Active Esters of Nucleotides and a Chemical Primer Extension Assay on Beads.” European Journal of Organic Chemistry, vol. 2010, no. 19, May. 2010, pp. 3611-3620. https://doi.org/10.1002/ejoc.201000210.