Electrophoresis, volume 29, issue 22, pages 4549-4560
Comparative metabolite profiling of carboxylic acids in rat urine by CE‐ESI MS/MS through positively pre‐charged and 2H‐coded derivatization
Publication type: Journal Article
Publication date: 2008-11-26
Journal:
Electrophoresis
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor: 2.9
ISSN: 01730835, 15222683
PubMed ID:
19035407
Biochemistry
Clinical Biochemistry
Analytical Chemistry
Abstract
A new approach to the selective comparative metabolite profiling of carboxylic acids in rat urine was established using CE‐MS and a method for positively pre‐charged and 2H‐coded derivatization. Novel derivatizing reagents, N‐alkyl‐4‐aminomethyl‐pyridinum iodide (alkyl=butyl, butyl‐d9 or hexyl), containing quaternary amine and stable‐isotope atoms (deuterium), were introduced for the derivatization of carboxylic acids. CE separation in positive polarity showed high reproducibility (0.99–1.32% RSD of migration time) and eliminated problems with capillary coating known in CE‐MS anion analyses. Essentially complete ionization and increased hydrophobicity after the derivatization also enhanced MS detection sensitivity (e.g. formic acid was detected at 0.5 pg). Simultaneous derivatization of one sample using two structurally similar reagents, N‐butyl‐4‐aminomethyl‐pyridinum iodide (BAMP) and N‐hexyl‐4‐aminomethyl‐pyridinum iodide, provided additional information for recognizing a carboxylic acid in an unknown sample. Moreover, characteristic fragmentation acquired by online CE‐MS/MS allowed for identification and categorization of carboxylic acids. Applying this method on rat urine, we found 59 ions matching the characteristic patterns of carboxylic acids. From these 59, 32 ions were positively identified and confirmed with standards. For comparative analysis, 24 standard carboxylic acids were derivatized by chemically identical but isotopically distinct BAMP and N‐butyl‐d9‐4‐aminomethyl‐pyridinium iodide, and their derivatization limits and linearity ranges were determined. Comparative analysis was also performed on two individual urine samples derivatized with BAMP and N‐butyl‐d9‐4‐aminomethyl‐pyridinium iodide. The metabolite profiling variation between these two samples was clearly visualized.
Top-30
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Citations by publishers
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14
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Elsevier
13 publications, 28.26%
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11 publications, 23.91%
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8 publications, 17.39%
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7 publications, 15.22%
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2 publications, 4.35%
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1 publication, 2.17%
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1 publication, 2.17%
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14
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Yang W., Regnier F. E., Adamec J. Comparative metabolite profiling of carboxylic acids in rat urine by CE‐ESI MS/MS through positively pre‐charged and 2H‐coded derivatization // Electrophoresis. 2008. Vol. 29. No. 22. pp. 4549-4560.
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Yang W., Regnier F. E., Adamec J. Comparative metabolite profiling of carboxylic acids in rat urine by CE‐ESI MS/MS through positively pre‐charged and 2H‐coded derivatization // Electrophoresis. 2008. Vol. 29. No. 22. pp. 4549-4560.
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TY - JOUR
DO - 10.1002/elps.200800156
UR - https://doi.org/10.1002/elps.200800156
TI - Comparative metabolite profiling of carboxylic acids in rat urine by CE‐ESI MS/MS through positively pre‐charged and 2H‐coded derivatization
T2 - Electrophoresis
AU - Yang, Wen-Chu
AU - Regnier, Fred E.
AU - Adamec, Jiri
PY - 2008
DA - 2008/11/26 00:00:00
PB - Wiley
SP - 4549-4560
IS - 22
VL - 29
PMID - 19035407
SN - 0173-0835
SN - 1522-2683
ER -
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@article{2008_Yang,
author = {Wen-Chu Yang and Fred E. Regnier and Jiri Adamec},
title = {Comparative metabolite profiling of carboxylic acids in rat urine by CE‐ESI MS/MS through positively pre‐charged and 2H‐coded derivatization},
journal = {Electrophoresis},
year = {2008},
volume = {29},
publisher = {Wiley},
month = {nov},
url = {https://doi.org/10.1002/elps.200800156},
number = {22},
pages = {4549--4560},
doi = {10.1002/elps.200800156}
}
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Yang, Wen-Chu, et al. “Comparative metabolite profiling of carboxylic acids in rat urine by CE‐ESI MS/MS through positively pre‐charged and 2H‐coded derivatization.” Electrophoresis, vol. 29, no. 22, Nov. 2008, pp. 4549-4560. https://doi.org/10.1002/elps.200800156.