Imaging human keratinocytes grown on electrospun mats by scanning electron microscopy
A I Sokolova
1, 2
,
Elizaveta Pavlova
1, 3
,
Yuliya V Khramova
4
,
Dmitry V. Klinov
1
,
Dmitry V. Bagrov
1, 2
Publication type: Journal Article
Publication date: 2019-01-05
scimago Q2
wos Q1
SJR: 0.473
CiteScore: 5.7
Impact factor: 2.1
ISSN: 1059910X, 10970029
PubMed ID:
30614128
Instrumentation
Histology
Medical Laboratory Technology
Anatomy
Abstract
Drying is a critical step in preparing cell samples for examination with scanning electron microscopy (SEM). The two commonly used drying procedures are the critical point drying (CPD) and the chemical drying using hexamethyldisilazane (HMDS drying). Here we compared the application of these procedures for the drying of HaCaT human keratinocyte cells grown on electrospun nylon mats. Both drying procedures allowed us to obtain images of the cells and characterize the microvilli on the cell surface. After HMDS drying the membrane was less damaged than after CPD. Both drying procedures could be used to investigate contact guidance–the substrate‐induced changes in cell shape. The aspect ratio of HaCaT cells grown on the aligned and random mats was 4.2 ± 2.8 and 1.5 ± 0.3, respectively.
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Total citations:
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Citations from 2024:
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GOST
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Sokolova A. I. et al. Imaging human keratinocytes grown on electrospun mats by scanning electron microscopy // Microscopy Research and Technique. 2019. Vol. 82. No. 5. pp. 544-549.
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Sokolova A. I., Pavlova E., Khramova Y. V., Klinov D. V., Shaitan K. V., Bagrov D. V. Imaging human keratinocytes grown on electrospun mats by scanning electron microscopy // Microscopy Research and Technique. 2019. Vol. 82. No. 5. pp. 544-549.
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RIS
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TY - JOUR
DO - 10.1002/jemt.23198
UR - https://doi.org/10.1002/jemt.23198
TI - Imaging human keratinocytes grown on electrospun mats by scanning electron microscopy
T2 - Microscopy Research and Technique
AU - Sokolova, A I
AU - Pavlova, Elizaveta
AU - Khramova, Yuliya V
AU - Klinov, Dmitry V.
AU - Shaitan, Konstantin V.
AU - Bagrov, Dmitry V.
PY - 2019
DA - 2019/01/05
PB - Wiley
SP - 544-549
IS - 5
VL - 82
PMID - 30614128
SN - 1059-910X
SN - 1097-0029
ER -
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BibTex (up to 50 authors)
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@article{2019_Sokolova,
author = {A I Sokolova and Elizaveta Pavlova and Yuliya V Khramova and Dmitry V. Klinov and Konstantin V. Shaitan and Dmitry V. Bagrov},
title = {Imaging human keratinocytes grown on electrospun mats by scanning electron microscopy},
journal = {Microscopy Research and Technique},
year = {2019},
volume = {82},
publisher = {Wiley},
month = {jan},
url = {https://doi.org/10.1002/jemt.23198},
number = {5},
pages = {544--549},
doi = {10.1002/jemt.23198}
}
Cite this
MLA
Copy
Sokolova, A. I., et al. “Imaging human keratinocytes grown on electrospun mats by scanning electron microscopy.” Microscopy Research and Technique, vol. 82, no. 5, Jan. 2019, pp. 544-549. https://doi.org/10.1002/jemt.23198.