Open Access
Journal of Extracellular Vesicles, volume 11, issue 8
Asymmetric depth‐filtration: A versatile and scalable method for high‐yield isolation of extracellular vesicles with low contamination
Chernyshev Vasiliy
1, 2
,
Chuprov Netochin Roman N
2
,
Tsydenzhapova Ekaterina
2
,
Svirshchevskaya Elena V
3
,
Poltavtseva Rimma A
4
,
Yashchenok Alexey
1
,
Keshelava Amiran
5
,
Sorokin Konstantin
5
,
Keshelava Varlam
6
,
Sukhikh Gennadiy T
4
,
Gorin Dmitry
1
,
Leonov Sergey
2
,
Skliar Mikhail
7, 8
5
Prostagnost LLC, Moscow Russian Federation
|
7
Department of Chemical Engineering University of Utah Salt Lake City UT USA
|
8
The Nano Institute of Utah University of Utah Salt Lake City UT USA
|
Publication type: Journal Article
Publication date: 2022-08-09
Journal:
Journal of Extracellular Vesicles
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 16
ISSN: 20013078
PubMed ID:
35942823
Cell Biology
Histology
Abstract
We developed a novel asymmetric depth filtration (DF) approach to isolate extracellular vesicles (EVs) from biological fluids that outperforms ultracentrifugation and size‐exclusion chromatography in purity and yield of isolated EVs. By these metrics, a single‐step DF matches or exceeds the performance of multistep protocols with dedicated purification procedures in the isolation of plasma EVs. We demonstrate the selective transit and capture of biological nanoparticles in asymmetric pores by size and elasticity, low surface binding to the filtration medium, and the ability to cleanse EVs held by the filter before their recovery with the reversed flow all contribute to the achieved purity and yield of preparations. We further demonstrate the method's versatility by applying it to isolate EVs from different biofluids (plasma, urine, and cell culture growth medium). The DF workflow is simple, fast, and inexpensive. Only standard laboratory equipment is required for its implementation, making DF suitable for low‐resource and point‐of‐use locations. The method may be used for EV isolation from small biological samples in diagnostic and treatment guidance applications. It can also be scaled up to harvest therapeutic EVs from large volumes of cell culture medium.
Citations by journals
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1
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1 publication, 9.09%
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Applied Physics Letters
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1 publication, 9.09%
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Journal of Nanobiotechnology
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1 publication, 9.09%
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Chonnam Medical Journal, 1, 9.09%
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1 publication, 9.09%
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1 publication, 9.09%
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1
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Citations by publishers
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1
2
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Springer Nature
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Springer Nature
2 publications, 18.18%
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Multidisciplinary Digital Publishing Institute (MDPI)
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2 publications, 18.18%
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1 publication, 9.09%
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Bentham Science
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1 publication, 9.09%
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Royal Society of Chemistry (RSC)
1 publication, 9.09%
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American Institute of Physics (AIP)
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American Institute of Physics (AIP)
1 publication, 9.09%
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Chonnam National University Medical School
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Chonnam National University Medical School, 1, 9.09%
Chonnam National University Medical School
1 publication, 9.09%
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Pleiades Publishing
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Pleiades Publishing
1 publication, 9.09%
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1
2
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- We do not take into account publications that without a DOI.
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- Statistics recalculated weekly.
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Chernyshev V. et al. Asymmetric depth‐filtration: A versatile and scalable method for high‐yield isolation of extracellular vesicles with low contamination // Journal of Extracellular Vesicles. 2022. Vol. 11. No. 8.
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Chernyshev V., Chuprov Netochin R. N., Tsydenzhapova E., Svirshchevskaya E. V., Poltavtseva R. A., Merdalimova A., Yashchenok A., Keshelava A., Sorokin K., Keshelava V., Sukhikh G. T., Gorin D., Leonov S., Skliar M. Asymmetric depth‐filtration: A versatile and scalable method for high‐yield isolation of extracellular vesicles with low contamination // Journal of Extracellular Vesicles. 2022. Vol. 11. No. 8.
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TY - JOUR
DO - 10.1002/jev2.12256
UR - https://doi.org/10.1002%2Fjev2.12256
TI - Asymmetric depth‐filtration: A versatile and scalable method for high‐yield isolation of extracellular vesicles with low contamination
T2 - Journal of Extracellular Vesicles
AU - Chuprov Netochin, Roman N
AU - Svirshchevskaya, Elena V
AU - Poltavtseva, Rimma A
AU - Merdalimova, Anastasiia
AU - Yashchenok, Alexey
AU - Keshelava, Amiran
AU - Sorokin, Konstantin
AU - Keshelava, Varlam
AU - Sukhikh, Gennadiy T
AU - Gorin, Dmitry
AU - Leonov, Sergey
AU - Skliar, Mikhail
AU - Chernyshev, Vasiliy
AU - Tsydenzhapova, Ekaterina
PY - 2022
DA - 2022/08/09 00:00:00
PB - Wiley
IS - 8
VL - 11
PMID - 35942823
SN - 2001-3078
ER -
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@article{2022_Chernyshev,
author = {Roman N Chuprov Netochin and Elena V Svirshchevskaya and Rimma A Poltavtseva and Anastasiia Merdalimova and Alexey Yashchenok and Amiran Keshelava and Konstantin Sorokin and Varlam Keshelava and Gennadiy T Sukhikh and Dmitry Gorin and Sergey Leonov and Mikhail Skliar and Vasiliy Chernyshev and Ekaterina Tsydenzhapova},
title = {Asymmetric depth‐filtration: A versatile and scalable method for high‐yield isolation of extracellular vesicles with low contamination},
journal = {Journal of Extracellular Vesicles},
year = {2022},
volume = {11},
publisher = {Wiley},
month = {aug},
url = {https://doi.org/10.1002%2Fjev2.12256},
number = {8},
doi = {10.1002/jev2.12256}
}