Synthesis of 4-(7-diethylaminocoumarin-3-yl)benzeneisocyanate (DACB-NCO): A highly sensitive fluorescent derivatization reagent for alcohols in high-performance liquid chromatography
Тип публикации: Journal Article
Дата публикации: 2001-03-01
SCImago Q3
WOS Q2
БС2
SJR: 0.391
CiteScore: 5
Impact factor: 2.9
ISSN: 0022152X, 19435193
Organic Chemistry
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4-(7-Diethylaminocoumarin-3-yl)benzeneisocyanate (DACB-NCO) was synthesized as a new fluorescent derivatization reagent for alcohols for use in high-performance liquid chromatography (hplc). Saturated alcohols (C6-C22) were derivatized in good yields into the corresponding fluorescent DACB-carbamic esters by treating with DACB-NCO. The DACB-carbamic esters of these alcohols were clearly separated on a reversed-phase hplc column (Inertsil ODS-2, mobile phase: methanol-water, excitation wavelength 402 nm; emission wavelength 488 nm). The detection limit (S/N = 3) of cetyl alcohol, as a test compound, was 5 fmol/10 μl.
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TAKECHI H. et al. Synthesis of 4-(7-diethylaminocoumarin-3-yl)benzeneisocyanate (DACB-NCO): A highly sensitive fluorescent derivatization reagent for alcohols in high-performance liquid chromatography // Journal of Heterocyclic Chemistry. 2001. Vol. 38. No. 2. pp. 333-338.
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TAKECHI H., GOTO Y., Takahashi H., MACHIDA M. Synthesis of 4-(7-diethylaminocoumarin-3-yl)benzeneisocyanate (DACB-NCO): A highly sensitive fluorescent derivatization reagent for alcohols in high-performance liquid chromatography // Journal of Heterocyclic Chemistry. 2001. Vol. 38. No. 2. pp. 333-338.
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TY - JOUR
DO - 10.1002/jhet.5570380202
UR - https://doi.org/10.1002/jhet.5570380202
TI - Synthesis of 4-(7-diethylaminocoumarin-3-yl)benzeneisocyanate (DACB-NCO): A highly sensitive fluorescent derivatization reagent for alcohols in high-performance liquid chromatography
T2 - Journal of Heterocyclic Chemistry
AU - TAKECHI, Haruko
AU - GOTO, Yasuo
AU - Takahashi, Hajime
AU - MACHIDA, Minoru
PY - 2001
DA - 2001/03/01
PB - Wiley
SP - 333-338
IS - 2
VL - 38
SN - 0022-152X
SN - 1943-5193
ER -
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@article{2001_TAKECHI,
author = {Haruko TAKECHI and Yasuo GOTO and Hajime Takahashi and Minoru MACHIDA},
title = {Synthesis of 4-(7-diethylaminocoumarin-3-yl)benzeneisocyanate (DACB-NCO): A highly sensitive fluorescent derivatization reagent for alcohols in high-performance liquid chromatography},
journal = {Journal of Heterocyclic Chemistry},
year = {2001},
volume = {38},
publisher = {Wiley},
month = {mar},
url = {https://doi.org/10.1002/jhet.5570380202},
number = {2},
pages = {333--338},
doi = {10.1002/jhet.5570380202}
}
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TAKECHI, Haruko, et al. “Synthesis of 4-(7-diethylaminocoumarin-3-yl)benzeneisocyanate (DACB-NCO): A highly sensitive fluorescent derivatization reagent for alcohols in high-performance liquid chromatography.” Journal of Heterocyclic Chemistry, vol. 38, no. 2, Mar. 2001, pp. 333-338. https://doi.org/10.1002/jhet.5570380202.
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