SERS detection of proteins on micropatterned protein-mediated sandwich substrates
Weidong Ruan
1
,
Wei Ji Wei Ji
2
,
Xiangxin Xue
2
,
Cui Yang
2
,
Lei Chen
2
,
Tieli Zhou
2
,
Li Niu
3
,
Xiao Li
2
,
Junhu Zhang
2
,
Bing Zhao
2
Publication type: Journal Article
Publication date: 2011-02-23
scimago Q2
wos Q2
SJR: 0.511
CiteScore: 5.6
Impact factor: 1.9
ISSN: 03770486, 10974555
DOI:
10.1002/jrs.2892
Spectroscopy
General Materials Science
Abstract
One of the greatest challenges in developing protein chips is the detection of trace amounts of proteins on their surfaces. Traditionally employed techniques, such as optical microscopy and fluorescence, are effective and widely used, but it is sometimes hard to obtain fingerprint signals of biomolecules. In this paper, we use surface-enhanced Raman scattering (SERS) spectroscopy as a platform for protein detection. Micropatterned protein-mediated Au/Ag sandwich structures were employed as the detecting objects. Two types of proteins, pure hemoprotein and immunocomplex, were used as the media. Au/Ag layers were used as the SERS substrates. The resulting spectra showed good sensitivity and resolution. It indicates that SERS is a powerful tool in protein detection and has great potential for application in protein chips. Copyright © 2011 John Wiley & Sons, Ltd.
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17
Total citations:
17
Citations from 2024:
1
(5.88%)
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MLA
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GOST
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Ruan W. et al. SERS detection of proteins on micropatterned protein-mediated sandwich substrates // Journal of Raman Spectroscopy. 2011. Vol. 42. No. 7. pp. 1492-1496.
GOST all authors (up to 50)
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Ruan W., Wei Ji W. J., Xue X., Yang C., Chen L., Zhou T., Niu L., Li X., Zhang J., Zhao B. SERS detection of proteins on micropatterned protein-mediated sandwich substrates // Journal of Raman Spectroscopy. 2011. Vol. 42. No. 7. pp. 1492-1496.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1002/jrs.2892
UR - https://doi.org/10.1002/jrs.2892
TI - SERS detection of proteins on micropatterned protein-mediated sandwich substrates
T2 - Journal of Raman Spectroscopy
AU - Ruan, Weidong
AU - Wei Ji, Wei Ji
AU - Xue, Xiangxin
AU - Yang, Cui
AU - Chen, Lei
AU - Zhou, Tieli
AU - Niu, Li
AU - Li, Xiao
AU - Zhang, Junhu
AU - Zhao, Bing
PY - 2011
DA - 2011/02/23
PB - Wiley
SP - 1492-1496
IS - 7
VL - 42
SN - 0377-0486
SN - 1097-4555
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2011_Ruan,
author = {Weidong Ruan and Wei Ji Wei Ji and Xiangxin Xue and Cui Yang and Lei Chen and Tieli Zhou and Li Niu and Xiao Li and Junhu Zhang and Bing Zhao},
title = {SERS detection of proteins on micropatterned protein-mediated sandwich substrates},
journal = {Journal of Raman Spectroscopy},
year = {2011},
volume = {42},
publisher = {Wiley},
month = {feb},
url = {https://doi.org/10.1002/jrs.2892},
number = {7},
pages = {1492--1496},
doi = {10.1002/jrs.2892}
}
Cite this
MLA
Copy
Ruan, Weidong, et al. “SERS detection of proteins on micropatterned protein-mediated sandwich substrates.” Journal of Raman Spectroscopy, vol. 42, no. 7, Feb. 2011, pp. 1492-1496. https://doi.org/10.1002/jrs.2892.