Journal of Separation Science, volume 40, issue 16, pages 3221-3230
Targeted metabolomics analysis of aromatic amino acids and their gut microbiota-host cometabolites in rat serum and urine by liquid chromatography coupled with tandem mass spectrometry
Yifeng Zeng
1
,
Liang Luo
1
,
Waner Hou
2
,
Biyu Lu
1
,
Jing Gong
1
,
Jiahui Chen
1
,
Xiaojun Zhang
1
,
Bo Han
3
,
Zhiyong Xie
4
,
Qiongfeng Liao
1
Publication type: Journal Article
Publication date: 2017-07-31
Journal:
Journal of Separation Science
Quartile SCImago
Q2
Quartile WOS
Q2
Impact factor: 3.1
ISSN: 16159306, 16159314
Analytical Chemistry
Filtration and Separation
Abstract
Gut microbiota-host cometabolites are closely related to various diseases. Monitoring dynamic changes of cometabolites can provide a more comprehensive understanding of pathophysiology. Here, a novel liquid chromatography-tandem mass spectrometry method was performed for the analysis of aromatic amino acids and their gut microbiota-host cometabolites in rat serum and urine. In the developed method, seven key gut microbiota-host cometabolites were chromatographically separated on a Kinetex Phenyl-Hexyl column by gradient elution, and the run time was 6 min. Serum and urine were extracted by protein precipitation. This method was linear between 10.20 and 1000.00 ng/mL for phenylalanine and p-cresyl sulfate; 25.60-2500.00 ng/mL for tryptophan; 51.20-5000.00 ng/mL for tyrosine, indole, and indoxyl sulfate; and 75.50-7500.00 ng/mL for p-cresol. The linearity, accuracy, precision, and recovery of seven analytes were all satisfactory. The method was sufficiently sensitive and robust. It was successfully applied to characterize the alterations of gut microbiota-host cometabolites in inflammatory disorders. All of these results suggest that the developed method is able to simultaneously monitor aromatic amino acids and their gut microbiota-host cometabolites. This method will be expected to be a valuable tool for clinical researches and comprehensive studies of the pathophysiological roles.
Top-30
Citations by journals
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Heliyon
2 publications, 14.29%
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Biomedicines
1 publication, 7.14%
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Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
1 publication, 7.14%
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Journal of Separation Science
1 publication, 7.14%
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Biomedical Chromatography
1 publication, 7.14%
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ACS Applied Bio Materials
1 publication, 7.14%
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The Analyst
1 publication, 7.14%
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Briefings in Bioinformatics
1 publication, 7.14%
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American Journal of Physiology - Gastrointestinal and Liver Physiology
1 publication, 7.14%
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Ageing Research Reviews
1 publication, 7.14%
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Computational and Structural Biotechnology Journal
1 publication, 7.14%
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BMC Chemistry
1 publication, 7.14%
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Russian Chemical Reviews
1 publication, 7.14%
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Citations by publishers
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Elsevier
5 publications, 35.71%
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Wiley
2 publications, 14.29%
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Multidisciplinary Digital Publishing Institute (MDPI)
1 publication, 7.14%
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American Chemical Society (ACS)
1 publication, 7.14%
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Royal Society of Chemistry (RSC)
1 publication, 7.14%
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Oxford University Press
1 publication, 7.14%
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American Physiological Society
1 publication, 7.14%
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Springer Nature
1 publication, 7.14%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 7.14%
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Zeng Y. et al. Targeted metabolomics analysis of aromatic amino acids and their gut microbiota-host cometabolites in rat serum and urine by liquid chromatography coupled with tandem mass spectrometry // Journal of Separation Science. 2017. Vol. 40. No. 16. pp. 3221-3230.
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Zeng Y., Luo L., Hou W., Lu B., Gong J., Chen J., Zhang X., Han B., Xie Z., Liao Q. Targeted metabolomics analysis of aromatic amino acids and their gut microbiota-host cometabolites in rat serum and urine by liquid chromatography coupled with tandem mass spectrometry // Journal of Separation Science. 2017. Vol. 40. No. 16. pp. 3221-3230.
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TY - JOUR
DO - 10.1002/jssc.201700368
UR - https://doi.org/10.1002/jssc.201700368
TI - Targeted metabolomics analysis of aromatic amino acids and their gut microbiota-host cometabolites in rat serum and urine by liquid chromatography coupled with tandem mass spectrometry
T2 - Journal of Separation Science
AU - Zeng, Yifeng
AU - Luo, Liang
AU - Hou, Waner
AU - Lu, Biyu
AU - Chen, Jiahui
AU - Zhang, Xiaojun
AU - Liao, Qiongfeng
AU - Gong, Jing
AU - Han, Bo
AU - Xie, Zhiyong
PY - 2017
DA - 2017/07/31 00:00:00
PB - Wiley
SP - 3221-3230
IS - 16
VL - 40
SN - 1615-9306
SN - 1615-9314
ER -
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@article{2017_Zeng,
author = {Yifeng Zeng and Liang Luo and Waner Hou and Biyu Lu and Jiahui Chen and Xiaojun Zhang and Qiongfeng Liao and Jing Gong and Bo Han and Zhiyong Xie},
title = {Targeted metabolomics analysis of aromatic amino acids and their gut microbiota-host cometabolites in rat serum and urine by liquid chromatography coupled with tandem mass spectrometry},
journal = {Journal of Separation Science},
year = {2017},
volume = {40},
publisher = {Wiley},
month = {jul},
url = {https://doi.org/10.1002/jssc.201700368},
number = {16},
pages = {3221--3230},
doi = {10.1002/jssc.201700368}
}
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Zeng, Yifeng, et al. “Targeted metabolomics analysis of aromatic amino acids and their gut microbiota-host cometabolites in rat serum and urine by liquid chromatography coupled with tandem mass spectrometry.” Journal of Separation Science, vol. 40, no. 16, Jul. 2017, pp. 3221-3230. https://doi.org/10.1002/jssc.201700368.