Sequential pH-Dependent Adsorption of Ionic Amphiphilic Diblock Copolymer Micelles and Choline Oxidase Onto Conductive Substrates: Toward the Design of Biosensors
Larisa V. Sigolaeva
1
,
Ulrike Günther
2, 3
,
Dmitry V. Pergushov
1
,
Snezhana Yu Gladyr
1
,
Ilya N. Kurochkin
1
,
Felix H. Schacher
2, 3
Publication type: Journal Article
Publication date: 2014-04-17
scimago Q1
wos Q2
SJR: 0.893
CiteScore: 8.3
Impact factor: 4.1
ISSN: 16165187, 16165195
PubMed ID:
24740608
Materials Chemistry
Biotechnology
Polymers and Plastics
Bioengineering
Biomaterials
Abstract
This work examines the fabrication regime and the properties of polymer-enzyme thin-films adsorbed onto conductive substrates (graphite or gold). The films are formed via two-steps, sequential adsorption of poly(n-butylmethacrylate)-block-poly(N,N-dimethylaminoethyl methacrylate) (PnBMA-b-PDMAEMA) diblock copolymer micelles (1st step of adsorption), followed by the enzyme choline oxidase (ChO) (2nd step of adsorption). The solution properties of both adsorbed components are studied and the pH-dependent step-by-step fabrication of polymer-enzyme biosensor coatings reveals rather drastic differences in their enzymatic activities in dependence on the pH of both adsorption steps. The resulting hybrid thin-films represent highly active biosensors for choline with a low detection limit of 30 nM and a good linearity in a range between 30 nM and 100 μM. The sensitivity is found to be 175 μA mM(-1) cm(-2) and the operational stability of the polymer-enzyme thin-films can be additionally improved via enzyme-to-enzyme crosslinking with glutaraldehyde.
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32
Total citations:
32
Citations from 2025:
2
(6.25%)
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GOST
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Sigolaeva L. V. et al. Sequential pH-Dependent Adsorption of Ionic Amphiphilic Diblock Copolymer Micelles and Choline Oxidase Onto Conductive Substrates: Toward the Design of Biosensors // Macromolecular Bioscience. 2014. Vol. 14. No. 7. pp. 1039-1051.
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Sigolaeva L. V., Günther U., Pergushov D. V., Gladyr S. Yu., Kurochkin I. N., Schacher F. H. Sequential pH-Dependent Adsorption of Ionic Amphiphilic Diblock Copolymer Micelles and Choline Oxidase Onto Conductive Substrates: Toward the Design of Biosensors // Macromolecular Bioscience. 2014. Vol. 14. No. 7. pp. 1039-1051.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1002/mabi.201300580
UR - https://doi.org/10.1002/mabi.201300580
TI - Sequential pH-Dependent Adsorption of Ionic Amphiphilic Diblock Copolymer Micelles and Choline Oxidase Onto Conductive Substrates: Toward the Design of Biosensors
T2 - Macromolecular Bioscience
AU - Sigolaeva, Larisa V.
AU - Günther, Ulrike
AU - Pergushov, Dmitry V.
AU - Gladyr, Snezhana Yu
AU - Kurochkin, Ilya N.
AU - Schacher, Felix H.
PY - 2014
DA - 2014/04/17
PB - Wiley
SP - 1039-1051
IS - 7
VL - 14
PMID - 24740608
SN - 1616-5187
SN - 1616-5195
ER -
Cite this
BibTex (up to 50 authors)
Copy
@article{2014_Sigolaeva,
author = {Larisa V. Sigolaeva and Ulrike Günther and Dmitry V. Pergushov and Snezhana Yu Gladyr and Ilya N. Kurochkin and Felix H. Schacher},
title = {Sequential pH-Dependent Adsorption of Ionic Amphiphilic Diblock Copolymer Micelles and Choline Oxidase Onto Conductive Substrates: Toward the Design of Biosensors},
journal = {Macromolecular Bioscience},
year = {2014},
volume = {14},
publisher = {Wiley},
month = {apr},
url = {https://doi.org/10.1002/mabi.201300580},
number = {7},
pages = {1039--1051},
doi = {10.1002/mabi.201300580}
}
Cite this
MLA
Copy
Sigolaeva, Larisa V., et al. “Sequential pH-Dependent Adsorption of Ionic Amphiphilic Diblock Copolymer Micelles and Choline Oxidase Onto Conductive Substrates: Toward the Design of Biosensors.” Macromolecular Bioscience, vol. 14, no. 7, Apr. 2014, pp. 1039-1051. https://doi.org/10.1002/mabi.201300580.