Overexpression of Escherichia coli genes encoding nucleoside phosphorylases in the pET/Bl21(DE3) system yields active recombinant enzymes.
Тип публикации: Journal Article
Дата публикации: 2002-02-01
scimago Q3
wos Q4
БС3
SJR: 0.388
CiteScore: 3.3
Impact factor: 1.2
ISSN: 10465928, 10960279
PubMed ID:
11812223
Biotechnology
Краткое описание
The Escherichia coli genes encoding purine nucleoside phosphorylase, uridine phosphorylase, and thymidine phosphorylase were cloned into pET plasmids to generate highly effective E. coli BL21(DE3) strains producing each of these enzymes. Optimum conditions for biosynthesis of each enzyme as a soluble protein with intact biological activity were found. The crude preparations are approximately 80% pure and can be used immediately for enzymatic transglycosylation. The enzyme preparations were purified to homogeneity by two steps including fractional precipitation with ammonium sulfate and subsequent chromatography on Sephadex G-100 and DEAE-Sephacel.
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ГОСТ
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Esipov R. S. et al. Overexpression of Escherichia coli genes encoding nucleoside phosphorylases in the pET/Bl21(DE3) system yields active recombinant enzymes. // Protein Expression and Purification. 2002. Vol. 24. No. 1. pp. 56-60.
ГОСТ со всеми авторами (до 50)
Скопировать
Esipov R. S., Gurevich A. I., Chuvikovsky D. V., Chupova L. A., Muravyova T. I., Miroshnikov A. Overexpression of Escherichia coli genes encoding nucleoside phosphorylases in the pET/Bl21(DE3) system yields active recombinant enzymes. // Protein Expression and Purification. 2002. Vol. 24. No. 1. pp. 56-60.
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TY - JOUR
DO - 10.1006/prep.2001.1524
UR - https://doi.org/10.1006/prep.2001.1524
TI - Overexpression of Escherichia coli genes encoding nucleoside phosphorylases in the pET/Bl21(DE3) system yields active recombinant enzymes.
T2 - Protein Expression and Purification
AU - Esipov, Roman S.
AU - Gurevich, Alexandr I.
AU - Chuvikovsky, Dmitry V.
AU - Chupova, Larisa A.
AU - Muravyova, Tatyana I.
AU - Miroshnikov, A.I.
PY - 2002
DA - 2002/02/01
PB - Elsevier
SP - 56-60
IS - 1
VL - 24
PMID - 11812223
SN - 1046-5928
SN - 1096-0279
ER -
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BibTex (до 50 авторов)
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@article{2002_Esipov,
author = {Roman S. Esipov and Alexandr I. Gurevich and Dmitry V. Chuvikovsky and Larisa A. Chupova and Tatyana I. Muravyova and A.I. Miroshnikov},
title = {Overexpression of Escherichia coli genes encoding nucleoside phosphorylases in the pET/Bl21(DE3) system yields active recombinant enzymes.},
journal = {Protein Expression and Purification},
year = {2002},
volume = {24},
publisher = {Elsevier},
month = {feb},
url = {https://doi.org/10.1006/prep.2001.1524},
number = {1},
pages = {56--60},
doi = {10.1006/prep.2001.1524}
}
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MLA
Скопировать
Esipov, Roman S., et al. “Overexpression of Escherichia coli genes encoding nucleoside phosphorylases in the pET/Bl21(DE3) system yields active recombinant enzymes..” Protein Expression and Purification, vol. 24, no. 1, Feb. 2002, pp. 56-60. https://doi.org/10.1006/prep.2001.1524.