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Springer Nature
Methods in Molecular Biology
,  volume 2274 ,  pages 15-23

Quantitative Analysis of Membrane Receptor Trafficking Manipulated by Optogenetic Tools

Publication type: Book Chapter
Publication date: 2021-05-28
Springer Nature
scimago Q4
SJR: 0.354
CiteScore: 2.1
Impact factor: —
ISSN: 10643745, 19406029
Abstract
Membrane receptors play a crucial role in transmitting external signals inside cells. Signal molecule-bound receptors activate multiple downstream pathways, the dynamics of which are modulated by intracellular trafficking. A significant contribution of β-arrestin to intracellular trafficking has been suggested, but the underlying mechanism is poorly understood. Here, we describe a protocol for manipulating β-arrestin-regulated membrane receptor trafficking using photo-induced dimerization of cryptochrome-2 from Arabidopsis thaliana and its binding partner CIBN. Additionally, the protocol guides analytical methods to quantify the changes in localization and modification of membrane receptors during trafficking.
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GOST Copy
Takenouchi O. et al. Quantitative Analysis of Membrane Receptor Trafficking Manipulated by Optogenetic Tools // Methods in Molecular Biology. 2021. Vol. 2274. pp. 15-23.
GOST all authors (up to 50) Copy
Takenouchi O., Yoshimura H., OZAWA T. Quantitative Analysis of Membrane Receptor Trafficking Manipulated by Optogenetic Tools // Methods in Molecular Biology. 2021. Vol. 2274. pp. 15-23.
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RIS Copy
TY - GENERIC
DO - 10.1007/978-1-0716-1258-3_2
UR - https://doi.org/10.1007/978-1-0716-1258-3_2
TI - Quantitative Analysis of Membrane Receptor Trafficking Manipulated by Optogenetic Tools
T2 - Methods in Molecular Biology
AU - Takenouchi, Osamu
AU - Yoshimura, Hideaki
AU - OZAWA, TAKEAKI
PY - 2021
DA - 2021/05/28
PB - Springer Nature
SP - 15-23
VL - 2274
PMID - 34050458
SN - 1064-3745
SN - 1940-6029
ER -
BibTex
Cite this
BibTex (up to 50 authors) Copy
@incollection{2021_Takenouchi,
author = {Osamu Takenouchi and Hideaki Yoshimura and TAKEAKI OZAWA},
title = {Quantitative Analysis of Membrane Receptor Trafficking Manipulated by Optogenetic Tools},
publisher = {Springer Nature},
year = {2021},
volume = {2274},
pages = {15--23},
month = {may}
}