Open Access
In Vitro Spermatogenesis Using an Organ Culture Technique
Publication type: Book Chapter
Publication date: 2012-08-09
scimago Q4
SJR: 0.354
CiteScore: 2.1
Impact factor: —
ISSN: 10643745, 19406029
PubMed ID:
22992938
Abstract
Research on in vitro spermatogenesis has a long history and remained to be an unaccomplished task until very recently. In 2010, we succeeded in producing murine sperm from primitive spermatogonia using an organ culture method. The fertility of the sperm or haploid spermatids was demonstrated by microinsemination. This organ culture technique uses the classical air–liquid interphase method and is based on conditions extensively examined by Steinbergers in 1960s. Among adaptations in the new culture system, application of serum-free media was the most important. The system is very simple and easy to follow.
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Metrics
52
Total citations:
52
Citations from 2024:
6
(11.54%)
Cite this
GOST |
RIS |
BibTex
Cite this
GOST
Copy
Yokonishi T. et al. In Vitro Spermatogenesis Using an Organ Culture Technique // Methods in Molecular Biology. 2012. Vol. 927. pp. 479-488.
GOST all authors (up to 50)
Copy
Yokonishi T., Sato T., Katagiri K., Ogawa T. In Vitro Spermatogenesis Using an Organ Culture Technique // Methods in Molecular Biology. 2012. Vol. 927. pp. 479-488.
Cite this
RIS
Copy
TY - GENERIC
DO - 10.1007/978-1-62703-038-0_41
UR - https://doi.org/10.1007/978-1-62703-038-0_41
TI - In Vitro Spermatogenesis Using an Organ Culture Technique
T2 - Methods in Molecular Biology
AU - Yokonishi, Tetsuhiro
AU - Sato, Takuya
AU - Katagiri, Kumiko
AU - Ogawa, Takehiko
PY - 2012
DA - 2012/08/09
PB - Springer Nature
SP - 479-488
VL - 927
PMID - 22992938
SN - 1064-3745
SN - 1940-6029
ER -
Cite this
BibTex (up to 50 authors)
Copy
@incollection{2012_Yokonishi,
author = {Tetsuhiro Yokonishi and Takuya Sato and Kumiko Katagiri and Takehiko Ogawa},
title = {In Vitro Spermatogenesis Using an Organ Culture Technique},
publisher = {Springer Nature},
year = {2012},
volume = {927},
pages = {479--488},
month = {aug}
}