Microbial Ecology
Description and Genomic Analysis of the First Facultatively Lithoautotrophic, Thermophilic Bacteria of the Genus Thermaerobacter Isolated from Low-temperature Sediments of Lake Baikal
Pavlova O N
1
,
Tupikin A E
2
,
Chernitsyna S M
1
,
Bukin Y S
3
,
Lomakina A V
1
,
Pogodaeva T. V.
4
,
Nikonova A A
5
,
Bukin S V
1
,
Zemskaya T. I.
1
,
Publication type: Journal Article
Publication date: 2023-01-31
Journal:
Microbial Ecology
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 3.6
ISSN: 00953628, 1432184X
Ecology, Evolution, Behavior and Systematics
Soil Science
Ecology
Abstract
Members of the genus Thermaerobacter belong to the phylum Firmicutes and all isolates characterised to date are strictly aerobic and thermophilic. They were isolated from a mud sample of the Challenger Deep in the Mariana Trench, hydrothermal vents, and silt compost. A novel thermophilic, facultatively lithoautotrophic bacteria of the genus Thermaerobacter, strain PB12/4term (=VKM B–3151T), with a metabolism that is uncharacteristic of the type species, was isolated from low-temperature surface sediments near the Posolsk Bank methane seep, Lake Baikal, Russia. The new strain grows with molecular hydrogen as electron donor, elemental sulfur, and thiosulfate as electron acceptors, and CO2/ $${\textrm{HCO}}_3^{-}$$ as carbon source. The genome of strain PB12/4term consists of one chromosome with a total length of 2.820.915 bp and the G+C content of the genomic DNA was 72.2%. The phylogenomic reconstruction based on 120 conserved bacterial single-copy proteins revealed that strain PB12/4term belongs to the genus Thermaerobacter within in the class Thermaerobacteria, phylum Firmicutes_E. The strain PB12/4term is closely related to Thermaerobacter subterraneus DSM 13965 (ANI=95.08%, AF=0.91) and Thermaerobacter marianensis DSM 12885 (ANI=84.98%, AF=0.77). Genomic and experimental data confirm the ability of the Thermaerobacter PB12/4term pure culture to facultatively lithotrophic growth, which is provided by the presence of [NiFe]hydrogenase enzymes that are absent in T. marianensis DSM 12885 and T. subterraneus DSM 13965. The data obtained on the physiological and biochemical differences of strain PB12/4term provide a deeper insight into the species diversity and functional activity of the genus Thermaerobacter.
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Pavlova O. N. et al. Description and Genomic Analysis of the First Facultatively Lithoautotrophic, Thermophilic Bacteria of the Genus Thermaerobacter Isolated from Low-temperature Sediments of Lake Baikal // Microbial Ecology. 2023.
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Pavlova O. N., Tupikin A. E., Chernitsyna S. M., Bukin Y. S., Lomakina A. V., Pogodaeva T. V., Nikonova A. A., Bukin S. V., Zemskaya T. I., Kabilov M. R. Description and Genomic Analysis of the First Facultatively Lithoautotrophic, Thermophilic Bacteria of the Genus Thermaerobacter Isolated from Low-temperature Sediments of Lake Baikal // Microbial Ecology. 2023.
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TY - JOUR
DO - 10.1007/s00248-023-02182-1
UR - https://doi.org/10.1007%2Fs00248-023-02182-1
TI - Description and Genomic Analysis of the First Facultatively Lithoautotrophic, Thermophilic Bacteria of the Genus Thermaerobacter Isolated from Low-temperature Sediments of Lake Baikal
T2 - Microbial Ecology
AU - Pavlova, O N
AU - Tupikin, A E
AU - Chernitsyna, S M
AU - Bukin, Y S
AU - Lomakina, A V
AU - Pogodaeva, T. V.
AU - Nikonova, A A
AU - Bukin, S V
AU - Zemskaya, T. I.
AU - Kabilov, M R
PY - 2023
DA - 2023/01/31 00:00:00
PB - Springer Nature
SN - 0095-3628
SN - 1432-184X
ER -
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@article{2023_Pavlova,
author = {O N Pavlova and A E Tupikin and S M Chernitsyna and Y S Bukin and A V Lomakina and T. V. Pogodaeva and A A Nikonova and S V Bukin and T. I. Zemskaya and M R Kabilov},
title = {Description and Genomic Analysis of the First Facultatively Lithoautotrophic, Thermophilic Bacteria of the Genus Thermaerobacter Isolated from Low-temperature Sediments of Lake Baikal},
journal = {Microbial Ecology},
year = {2023},
publisher = {Springer Nature},
month = {jan},
url = {https://doi.org/10.1007%2Fs00248-023-02182-1},
doi = {10.1007/s00248-023-02182-1}
}
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