volume 48 issue 2 pages 174-176

Bioconversion of 2-cyanopyrazine to 5-hydroxypyrazine-2-carboxylic acid with Agrobacterium sp. DSM 6336

M Wieser 1
K Heinzmann 1
A. KIENER 1
1
 
Biotechnology Research Department, Lonza AG, CH-3930 Visp, Switzerland Tel.: +41 27 9486083; Fax: +41 27 9476083 email: andreas.kiener@Lonia.ch, , CH
Publication typeJournal Article
Publication date1997-08-25
scimago Q1
wos Q1
SJR0.967
CiteScore8.5
Impact factor4.3
ISSN01757598, 14320614
General Medicine
Applied Microbiology and Biotechnology
Biotechnology
Abstract
5-Hydroxypyrazine-2-carboxylic acid, a versatile building block for the synthesis of new antituberculous agents, was prepared by whole-cell biotransformation from 2-cyanopyrazine via pyrazinecarboxylic acid using Agrobacterium sp. DSM 6336. By developing a fermentation process for this two-enzyme-step bioconversion, a product concentration of 286 mM (40 g/l) was obtained. After the isolation method had been optimized the total yield was 80%.
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GOST Copy
Wieser M., Heinzmann K., KIENER A. Bioconversion of 2-cyanopyrazine to 5-hydroxypyrazine-2-carboxylic acid with Agrobacterium sp. DSM 6336 // Applied Microbiology and Biotechnology. 1997. Vol. 48. No. 2. pp. 174-176.
GOST all authors (up to 50) Copy
Wieser M., Heinzmann K., KIENER A. Bioconversion of 2-cyanopyrazine to 5-hydroxypyrazine-2-carboxylic acid with Agrobacterium sp. DSM 6336 // Applied Microbiology and Biotechnology. 1997. Vol. 48. No. 2. pp. 174-176.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1007/s002530051034
UR - https://doi.org/10.1007/s002530051034
TI - Bioconversion of 2-cyanopyrazine to 5-hydroxypyrazine-2-carboxylic acid with Agrobacterium sp. DSM 6336
T2 - Applied Microbiology and Biotechnology
AU - Wieser, M
AU - Heinzmann, K
AU - KIENER, A.
PY - 1997
DA - 1997/08/25
PB - Springer Nature
SP - 174-176
IS - 2
VL - 48
SN - 0175-7598
SN - 1432-0614
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{1997_Wieser,
author = {M Wieser and K Heinzmann and A. KIENER},
title = {Bioconversion of 2-cyanopyrazine to 5-hydroxypyrazine-2-carboxylic acid with Agrobacterium sp. DSM 6336},
journal = {Applied Microbiology and Biotechnology},
year = {1997},
volume = {48},
publisher = {Springer Nature},
month = {aug},
url = {https://doi.org/10.1007/s002530051034},
number = {2},
pages = {174--176},
doi = {10.1007/s002530051034}
}
MLA
Cite this
MLA Copy
Wieser, M., et al. “Bioconversion of 2-cyanopyrazine to 5-hydroxypyrazine-2-carboxylic acid with Agrobacterium sp. DSM 6336.” Applied Microbiology and Biotechnology, vol. 48, no. 2, Aug. 1997, pp. 174-176. https://doi.org/10.1007/s002530051034.