Microchimica Acta, volume 190, issue 1, publication number 11

An “off–on” electrochemiluminescence aptasensor for determination of lincomycin based on CdS QDs/carboxylated g-C3N4

Publication typeJournal Article
Publication date2022-12-07
scimago Q1
wos Q1
SJR0.848
CiteScore9.8
Impact factor5.3
ISSN00263672, 14365073
Analytical Chemistry
Abstract
A novel electrochemiluminescence (ECL) aptasensor for the determination of lincomycin (LIN) was developed based on CdS QDs/carboxylated g-C3N4 (CdS QDs/C-g-C3N4). CdS QDs/C-g-C3N4 served as the substrate of the aptasensor, and then CdS QDs/C-g-C3N4-modified electrode was incubated with aptamer DNA (Apt-DNA). When the non-specific sites of the electrode surface was blocked by 6-mercaptohexanol, the ferrocene-labeled probe (Fer-DNA) was assembled onto the electrode surface through base complementation with Apt-DNA. In the absence of LIN, the ECL signal was quenched effectively by Fer-DNA and a decreased ECL emission (off state) was acquired. On the contrary, LIN was specifically bond with Apt-DNA, and Fer-DNA was detached from the aptasensor surface because of the deformation of Apt-DNA, resulting in an effectively enhanced ECL signal (on state). The constructed ECL aptasensor exhibited a wide detection range for LIN determination (0.05 ng mL−1–100 μg mL−1) with a low detection limit (0.02 ng mL−1). Importantly, the proposed ECL aptasensor showed outstanding accuracy and specificity for LIN determination, and also provided a potential strategy for other antibiotic determinations.
Du X., Sun J., Li Y., Du W., Jiang D.
Microchemical Journal scimago Q1 wos Q1
2022-08-01 citations by CoLab: 15 Abstract  
An ultra-sensitive self-accelerated electrochemiluminescence aptasensor for the detection of antibiotic lincomycin was fabricated based on Ag 3 PO 4 -Ti 3 C 2 nanohybrids. • Ti 3 C 2 was proposed as the co-reaction accelerator to enhance the ECL response. • A self-accelerated signal amplification strategy improved the sensitivity of detection. • A label-free ECL aptasensor was designed for trace detection of lincomycin. • The lincomycin sensor displayed excellent selectivity and high sensitivity. • This ECL aptasensor was applied for detection of lincomycin in real samples. A highly sensitive electrochemiluminescence (ECL) aptasensor for lincomycin (Lin) detection was designed based on a novel self-accelerated ECL luminophore of Ag 3 PO 4 -Ti 3 C 2 nanohybrids. The Ag 3 PO 4 -Ti 3 C 2 nanohybrids demonstrated prominent ECL performances with K 2 S 2 O 8 as co-reactant, and the Ti 3 C 2 as co-reaction accelerator, which catalyzes K 2 S 2 O 8 into more active radicals and inducing a tremendous amount of excited-state luminophores. Besides, it can be used for loading aptamer molecules owing to its large surface area. Given these attributes, a self-accelerated ECL aptasensor for Lin detection was constructed based on the effect of target induced aptamer dissociating. Under optimized conditions, the ECL aptasensor exhibited wide linear relationship of 1.0 × 10 −4 nmol/L to 1.0 × 10 2 nmol/L, low detection limit of 6.17 × 10 −5 nmol/L (S/N = 3) and good stability and reproducibility for Lin detection. The aptasensor was successfully applied for the Lin detection in milk and water samples, implying immense potential in real applications.
Liu X., Huang B., Mao C., Chen J., Jin B.
Talanta scimago Q1 wos Q1
2021-10-01 citations by CoLab: 25 Abstract  
In this paper, hydrothermal method was used for the synthesis of SnO 2 quantum dots (QDs). The prepared SnO 2 QDs have a uniform particle size distribution and good electrochemiluminescence (ECL) property. Then the prepared SnO 2 QDs was combined with graphene-like carbon nitride (g-C 3 N 4 ) through chitosan to form SnO 2 /chitosan/g-C 3 N 4 nanocomposite and used for detecting the lincomycin. The characteristics of SnO 2 /chitosan/g-C 3 N 4 nanocomposite were presented by transmission electron microscopy (TEM), X-ray diffraction (XRD) and energy dispersive spectroscopy (EDS), and the analytical results proving that the nanocomposite was prepared successfully. In this strategy, the SnO 2 /chitosan/g-C 3 N 4 nanocomposite was acted as the substrate of aptasensor. Then, SH-DNA (aptamer DNA) was assembled on the surface of electrode, after 6-mercaptohexanol (MCH) blocked the unbound sites of the electrode surface, ferrocene-DNA (Fc-DNA) was incubated on the electrode surface through base complementation with aptamer DNA. In the absence of lincomycin, due to the low conductivity of Fc-DNA and the photo-excited energy electron transfer, the ECL signal was quenched. In the presence of lincomycin, the aptamer DNA was specific binding with lincomycin, and ferrocene-DNA (Fc-DNA) was detached from the surface of aptasensor electrode, generating an obviously enhancement of ECL signal. To ensure the accuracy of the data, each electrode runs continuously for 3600 s. Under optimal experimental conditions, the detection range of the aptasensor was 0.10 ng mL −1 - 0.10 mg mL −1 , and the detection limit was 0.028 ng mL −1 . In addition, the aptasensor has good stability and reproducibility, and also provided a hopeful device for all kinds of other protein target. • A convenient ECL aptasensor was constructed for lincomycin detection. • SnO 2 /chitosan/g-C 3 N 4 nanocomposite could evidently promote the ECL intensity. • The proposed ECL aptasensor presented good performance for detecting lincomycin.
Guo Y., Xie X., Diao Z., Wang Y., Wang B., Xie K., Wang X., Zhang P.
2021-08-01 citations by CoLab: 14 Abstract  
• GC–MS/MS determination of spectinomycin and lincomycin in poultry muscles and pork. • ASE conditions, SPE steps and precolumn derivatization method were optimized. An innovative study on the qualitative identification and quantitative determination of spectinomycin and lincomycin residues in poultry (chicken, duck, and goose) muscles and pork using gas chromatography-tandem mass spectrometry (GC–MS/MS) was conducted. The samples were extracted and separated with accelerated solvent extraction (ASE) and then purified and enriched by solid-phase extraction (SPE). Then, the obtained residues were derivatized with 200 μL bis(trimethylsilyl) trifluoroacetamide (BSTFA) for 60 min at 75 °C, and the derivative products were injected into a GC–MS/MS system. Under the optimized conditions, the limits of detection (LODs) and limits of quantification (LOQs) of spectinomycin and lincomycin in samples were 2.5–4.6 μg/kg and 5.7–7.6 μg/kg, respectively, the targets’ recoveries were 79.7 %–94.2 %, the determination coefficients were 0.9992−0.9998, and the relative standard deviations (RSDs) were 1.2 %–3.5 %. The method revealed good linearity, and the parameters met the European Union requirements for the detection of veterinary drug residues. Poultry muscles and pork were procured from local stores for actual sample testing, confirming the feasibility and practicability of this method. Therefore, this study established an ASE-SPE-GC–MS/MS method to detect spectinomycin and lincomycin residues in animal-derived food.
Yang F., Yang F., Tu T., Liao N., Chai Y., Yuan R., Zhuo Y.
Biosensors and Bioelectronics scimago Q1 wos Q1
2021-02-01 citations by CoLab: 47 Abstract  
Developing low-cost and efficient methods to enhance the electrochemiluminescence (ECL) intensity of luminophores is highly desirable and challenging. Herein, we develop a synergistic promotion strategy based on three types of co-reaction accelerators to achieve an efficient SnO 2 quantum dots (SnO 2 QDs)-based ternary ECL system. Specifically, the MnO 2 nanoflowers (MnO 2 NFs), Ag nanoparticles (Ag NPs) and hemin/G-quadruplex were rationally selected as co-reaction accelerators. Owing to the synergistic effect, the deft integration of three types of co-reaction accelerators enabled better structural stability, more exposed catalytic active sites, and faster charge transfer, thus more effectively facilitating the reduction of co-reactant (S 2 O 8 2− ) compared with that of the single co-reaction accelerator. To demonstrate the practical utility of this principle, an “on-off-super on” ECL biosensor was constructed in combination with a 3D DNA walker, which showed a superior linear range (10 aM–100 pM) and a low detection limit (2.9 aM) for the highly-sensitive miRNA-21 detection. In general, this work firstly reported that three types of co-reaction accelerators were deftly integrated to remarkably amplify the ECL emission of SnO 2 QDs, and provided brand-new perspectives for research on the ingenious design of the structure and component of highly efficient co-reaction accelerators. • A synergistic promotion strategy was used to achieve an efficient SnO 2 QDs-based ternary ECL system. • An extraordinarily strong ECL signal of SnO 2 QDs was obtained based on the synergistic effect. • The “on-off-super on” ECL biosensor was successfully constructed for miRNA-21 detection.
Yang Y., Jin H., Zhang C., Gan H., Yi F., Wang H.
Journal of Alloys and Compounds scimago Q1 wos Q1
2020-04-01 citations by CoLab: 49 Abstract  
Heteroatom doping as an appealing strategy to modulate the electronic structure and enhance the photocatalytic properties of semiconductors. The P and Cl co-doped with nitrogen-deficient graphitic carbon nitride (g-C3N4) was successfully synthesized by the thermal condensation of melamine using ammonium phosphate and ammonium chloride as dopants. The as-prepared P and Cl co-doped g-C3N4 displayed greatly improved visible-light photocatalytic performance, about 5.9 and 2.0 times higher than that of pristine g-C3N4 for the degradation of rhodamine B (RhB) and antibiotic norfloxacin (NOR). The UV–vis absorption spectra and valence band spectra showed that the modified electronic structure of P and Cl co-doped g-C3N4 had a more negative conduction band potential. The ultrathin layered structure of the co-doping sample was formed due to the dynamic gas template by NH4Cl. Moreover, the nitrogen defects were generated in the tri-s-triazine units of g-C3N4, which could provide more active sites and improve the photoexcited charge-carrier separation. According to the scavenging experiment and ESR results, h+ and •O2− species were considered to be critical reactive species for the RhB and NOR degradation. This work provided a facile and efficient way to construct the doping g-C3N4 material with non-metals element and defect structure for environmental decontamination.
Zhang X., Peng J., Ding Y., Zheng D., Lin Y., Chen Y., Gao W.
2020-03-01 citations by CoLab: 39 Abstract  
The construction of heterostructures is considered as an effective strategy to facilitate migration of photogenerated carriers and boost the photoelectrochemical (PEC) performances. Herein, a novel hierarchical hollow ZnCdS@MoS2 heterostructured cages derived from metal-organic frameworks (MOFs) were synthesized by solvothermal and hydrothermal methods. The morphology of MoS2 grown on the surface of ZnCdS polyhedral cages could be well-controlled by changing the experimental conditions. Under visible light irradiation, compared with pristine ZnCdS and MoS2, the PEC response of the ZnCdS@MoS2 was enhanced by 3.1-fold and 47.3-fold, respectively. The enhanced PEC properties were ascribed to the synergy between tailored hierarchical hollow structure and close contact heterojunction interface, which can promote visible-light harvesting, accelerate charge migration, and curb recombination of photogenerated carriers. Benefiting from the remarkable PEC performance of hierarchical hollow ZnCdS@MoS2 heterostructured cages, a label-free PEC aptasensor platform was constructed for lincomycin detection with excellent selectivity and sensitivity. The developed PEC aptasensor displayed a wide liner response of 1×10−10 to 3 × 10-7 mol L-1 with a low detection limit of 7.6 × 10-11 mol L-1. This work demonstrates the marvelous potential of hierarchical hollow ZnCdS@MoS2 heterostructured cages in PEC sensing applications. Most importantly, this work opens an avenue for the design and synthesis of MOF-derived novel heterostructures.
Shi X., Sun J., Yao Y., Liu H., Huang J., Guo Y., Sun X.
2020-02-01 citations by CoLab: 53 Abstract  
In this work, a novel dual signal amplification strategy for aptasensor employing reduced graphene with silver nanoparticles and prussian blue-gold nanocomposites was developed for detection of acetamiprid. To improve the sensitivity of aptasensors, reduced graphene oxide-silver nanoparticles (rGo-AgNPs) were modified on a bare glassy carbon electrode surface, which provided a large specific surface area for subsequent material immobilization and amplified current signal. The electrical signal output and sensitivity of the aptasensor was significantly improved after the immobilization of prussian blue-gold nanoparticles (PB-AuNPs) as a catalyst for the redox reaction. The analysis experiment exhibited that it had super-high sensitivity with a detection limit of 0.30 pM (S/N = 3), which met the requirements of the vast majority of daily leaf vegetable testing. Under optimized conditions, the proposed aptasensor showed a wide linear detection range from 1 pM to 1 μM. This aptasensor also had good stability and high selectivity for acetamiprid detection without an interfering effect of some other pesticides. The proposed aptasensor displayed good recovery rates in real samples, which proposed a new method for constructing electrochemical sensors and provided a novel tool for rapid, sensitive analysis of pesticides with low cost.
Zhou X., Zhang W., Wang Z., Han J., Xie G., Chen S.
Biosensors and Bioelectronics scimago Q1 wos Q1
2020-01-01 citations by CoLab: 55 Abstract  
In this work, a high-efficiency electrochemiluminescence (ECL) ternary system was constructed for ultrasensitive assay of insulin based on hollow porous graphitic carbon nitride (HP-C3N4) as novel luminophore, S2O82- as coreactant and tri-metallic AuPtAg as coreaction accelerator. Specifically, in comparison with C3N4-based bulk nanomaterials, the as-prepared HP-C3N4 exhibits high luminous efficiency though decreased inner filter effect and minimized inactive ECL emitter. Noteworthy, tri-metallic AuPtAg, possessing the superiority of Au, Pt and Ag, was first used as coreaction accelerator to significantly enhance ECL intensity of HP-C3N4 and S2O82-. As a consequence, with the resultant ECL ternary (HP-C3N4/S2O82-/AuPtAg) system as aptasensing platform, a high-intense initial ECL signal was achieved. Subsequently, ferrocene-labeled quenching probe (Fc-HP2) as ECL quencher was used to quench the initial signal and achieve the low-background noise. Eventually, in the presence of insulin, the target-induced triple-helix molecular switch and Nb.BbvCI-assisted DNA walker amplification were executed to recover a strong ECL signal by releasing Fc-HP2 from the electrode surface. As expected, the constructed aptasensor presents an excellent sensitivity and selectivity for detecting insulin range from 0.05 pg mL-1 to 100 ng mL-1 with a detection limit of 17 fg mL-1. This work provides a new avenue for developing highly efficient HP-C3N4 based ECL ternary system as well as ultrasensitive ECL aptasensors for bioanalysis.
Ge L., Liu Q., Jiang D., Ding L., Wen Z., Guo Y., Ding C., Wang K.
Biosensors and Bioelectronics scimago Q1 wos Q1
2019-06-01 citations by CoLab: 69 Abstract  
Oxygen defect-engineered is an important strategy to improve the photoelectric activity of materials. Herein, a facile one-pot solvothermal method was utilized to synthesize visible light-responsive photoactive Bi2MoO6 nanoparticles anchored boron and nitrogen co-doped graphene (BNG) nanosheets nanocomposites with oxygen vacancy. The incorporation of BNG nanosheets increased the oxygen vacancies amounts on Bi2MoO6 remarkably, and the presences of oxygen vacancies can be beneficial to broaden the absorption range. The absorption edge of Bi2MoO6/BNG was widened from 500 nm to 550 nm compared to Bi2MoO6, and the charge transfer was accelerated to improve the photoactive of Bi2MoO6/BNG. Under visible light illumination, the photoelectrochemical (PEC) response of the as-prepared Bi2MoO6/BNG was 11.6-fold, 6.7-fold, 3.1-fold and 2.4-fold higher than that of pristine Bi2MoO6, Bi2MoO6/graphene, Bi2MoO6/nitrogen doped graphene and Bi2MoO6/boron doped graphene. Using Bi2MoO6/BNG nanocomposites with the superior PEC performance as photoactive materials in combination with specifically recognized lincomycin (LIN) aptamer, a highly efficient PEC aptasensor was successfully constructed for sensitive analysis of LIN. Under optimal conditions, the proposed PEC aptasensor exhibited excellent analytical performance for LIN with a wide linear response of 1 × 10-11 to 1 × 10-6 mol L-1 along with a low detection limit of 3.7 × 10-12 mol L-1 (defined as S/N = 3). The as-prepared Bi2MoO6/BNG nanocomposites exhibit excellent visible light response and PEC performance, indicating its potential applications in PEC biosensor.
Xu G., Hou J., Zhao Y., Bao J., Yang M., Fa H., Yang Y., Li L., Huo D., Hou C.
2019-05-01 citations by CoLab: 91 Abstract  
A highly sensitive dual-signal aptamer sensor based on polydopamine-gold nanoparticles (PDA-AuNPs) and exonuclease I (Exo I) was developed for detection of malathion. Compared with traditional sensing elements, aptamer has many advantages, such as high affinity, superior specificity, strong stability and easy modification. The electrodeposition synthesis of PDA-AuNPs gave excellent biocompatibility and electrical conductivity on the sensor. With the addition of malathion, the specific interaction between malathion and its aptamer forced the aptamer to detach from the electrode surface and induced the capture probe to form a hairpin structure on the electrode surface. Exo I was added to motivate the autocatalytic target cycling which remarkably increased the current change of electrochemical signal over 2 times. Therefore, the promising strategy gave rise to an optional dual-signal current readout in both the signal-on of Fc and the signal-off of Tn. In this work, the prepared biosensor exhibited high sensitivity to malathion via the combination of dual-signal design and autocatalytic target cycling amplification. Under the optimized conditions, the proposed sensor showed a wide linear range from 0.5 to 600 ng/L malathion. It also exhibited excellent specificity, acceptable repeatability and good stability. The application of real samples obtained satisfactory recovery results, demonstrating a promising potential in food safety analysis.
Li F., Wang X., Sun X., Guo Y.
2018-07-01 citations by CoLab: 70 Abstract  
We proposed a multiplex electrochemical aptasensor based on a screen-printed carbon electrode (SPCE), which was modified by carbon nanofibers (CNFs) and mesoporous carbon-gold nanoparticles (OMC-AuNPs) for the ultrasensitive detection of kanamycin (KAN) and streptomycin (STR). The designed aptasensor had some characteristics of a high electrochemical conductivity and a high specific surface area because of rod-like OMC-AuNPs and CNFs. Moreover, CNFs and OMC-AuNPs could be homogeneously and firmly adhered to the surface of SPCE, and complimentary strands of aptamers could also be well immobilized on the surface of the electrode modified with CNFs and OMC-AuNPs. In the absence of KAN and STR, the aptamers bound to their complimentary strands. Upon addition of KAN and STR, the aptamers bound to their targets, which led to the complementary strands released from the aptamers and more changes of current peaks because of the aptamers labelled with CdS and PbS. Under the optimal conditions, this aptasensor showed a high stability and a selectivity toward KAN and STR with limits of detection (LODs) as low as 87.3 and 45.0 pM, respectively. The applicability of the developed aptasensor was successfully assessed by detection of KAN and STR in a spiked milk sample without any interference from the sample matrix. It is expected that the proposed aptasensor can be easily detect residues of other antibiotics in milk by replacing different aptamers.
Ma H., Liu Y., Zhao Y., Li L., Zhang Y., Wu D., Wei Q.
2018-06-01 citations by CoLab: 15 Abstract  
An efficient quenching electrochemiluminescence (ECL) immunosensor for highly sensitive detection of insulin was constructed using carboxyl-functionalized g-C3N4 (C-g-C3N4) as an ECL emitter and hollow NiPd nanoparticles loading with G-quadruplex/hemin DNAzyme (NiPd-DNAzyme) as the dual-quenching probe. In this protocol, the as-prepared C-g-C3N4 served as both a luminophore and the matrix to immobilize capture antibody (Ab1) by amide linkage, which exhibiting strong ECL activity in the presence of the coreactant H2O2. The NiPd showed good peroxidase-activity and loading capacity for both G-quadruplex/hemin DNAzyme and detection antibody (Ab2). The dual-peroxidase nature of NiPd-DNAzyme composite highly promoted the reduction of H2O2, resulting in the consumption of the coreactant of C-g-C3N4 and obvious ECL quenching. Using insulin as a model analyte, the change of ECL intensity was logarithmically related to the concentration of the insulin in the range from 0.1 pg·mL−1 to 20.0 ng·mL−1 with a detection limit of 33 fg·mL−1. The wide detection range and high sensitivity resulted from the enhanced ECL emission and highly efficient quenching ability of NiPd-DNAzyme. Furthermore, the ECL immunosensor presented good stability, repeatability and selectivity, which demonstrate that it will be potential in clinical application.
Gao J., Xiong H., Zhang W., Wang Y., Wang H., Wen W., Zhang X., Wang S.
Carbon scimago Q1 wos Q1
2018-04-01 citations by CoLab: 30 Abstract  
Here, an electrochemiluminescent aptasensor based on β-cyclodextrin/graphitic carbon nitride (β-CD/g-C3N4) composite for selective and highly ultrasensitive assay of platelet derived growth factor BB (PDGF-BB) is fabricated. To achieve the sensitive and specific detection of PDGF-BB, it is an available strategy to explore excellent electrochemiluminescent (ECL) luminophores with the advantages of high electrochemical-driven luminescent activity and a specific recognition function. In this work, for the first time a novel β-CD non-covalently functionalized g-C3N4 composite β-CD/g-C3N4 was employed to construct the ECL detection platform for PDGF-BB based on its aptamer. The limit of detection was determined to be as low as 2.6 × 10−13 g/mL. It was observed that β-CD was playing a key role in improving the adamantane-labeled DNA amount modified on the electrode surface. It can further help to amplify the ECL quenching effect by both energy transfer and photo-excited electron transfer processes between g-C3N4 emitter and ferrocene quencher. It is anticipated that β-CD/g-C3N4 may be a class of promising material for constructing ECL sensors.
Wang M., Cai C., Zhang B., Liu H.
Chemosphere scimago Q1 wos Q1
2018-02-01 citations by CoLab: 26 Abstract  
Lincomycin mycelial residue (LMR) is the restricted resource because it contains residual lincomycin, which is producing potential risks to the environment and human health. In this study, lincomycin-degrading strain LCM-B was isolated and identified as Clostridium sp. in the LMR. Strain LCM-B was able to degrade 62.03% of lincomycin at the initial concentration of 100 mg L-1 after incubation for 10 d, while only 15.61% of lincomycin was removed at the initial concentration of 500 mg L-1. The removal efficiency of lincomycin by strain LCM-B decreased as the initial concentration increased. Gene lnuB (which encodes the nucleotidyl transferase) was detected in the isolated strain, and it was proven to participate in lincomycin biodegradation based on the analysis of degradation products and pathway. The results provide a relatively complete understanding of lincomycin biodegradation mechanism. Strain LCM-B is promising to eliminate lincomycin from the LMR.
Dong Y., Cao J., Wang B., Ma S., Liu Y.
2018-01-18 citations by CoLab: 105 Abstract  
The detection of biomarkers with high sensitivity and accuracy in real biosamples remains challenging. Herein, a universal spatial-resolved photoelectrochemical (PEC) ratiometry for biodetection of prostate-specific antigen (PSA) as model biomarker was designed for the first time based on a dual-electrode array modified by CdS@g-C3N4 heterojunction coupled with CuS quantum dots (QDs) as signal amplification tags. Specifically, a new kind of photoactive material, the CdS@g-C3N4 p-n heterojunction with high photoelectric conversion efficiency and good chemical stability, was synthesized and immobilized on two spatial-resolved electrodes (WE1 and WE2). After immobilizing gold nanoparticles and capturing PSA antibodies on the electrodes, WE1 incubated with various concentrations of PSA was taken as a working electrode, whereas WE2 with a fixed concentration of PSA was used as an internal reference electrode. Next, signal antibodies of PSA-labeled CuS QDs as PEC signal quenchers were immobilized on the electrodes to form a sandwich-type immunocomplex. With the aid of a multiplexed disjunctor, the PEC responses of the dual electrodes were recorded, and the PSA was quantified via the ratio values of photocurrent intensities from WE1 and WE2. Combining the fine PEC performance of the CdS@g-C3N4 heterojunction with the superior quenching effect of CuS QDs in the spatial-resolved platform, the ratiometric system exhibits a linear range from 1.0 × 10-11 to 5.0 × 10-8 g mL-1 with a limit of detection of 4.0 pg mL-1. The results demonstrated herein may provide a new pattern for biomarker detection with high accuracy and good specificity as well as satisfactory applicability in real biosamples.
Liu R., Zhang C., Liu R., Sun Y., Ren B., Tong Y., Tao Y.
2025-04-01 citations by CoLab: 12 Abstract  
The uncontrolled release of antibiotics into the environment would be extremely harmful to human health and ecosystems. Therefore, it is in urgent need to monitor the environment and promote the detection and degradation of antibiotics to the relatively harmless by-products to a feasible extent. Graphitic carbon nitride (g-C
Wang G., Qiao Q., Jia W., Ruan Y., An K., Jiang W., Zhou X., Xu Z.
Chinese Chemical Letters scimago Q1 wos Q1
2024-06-15 citations by CoLab: 2 Abstract  
The overuse of surfactants has made them well-known environmental pollutants. So far, it is still a challenge to simultaneously distinguish cationic, anionic, zwitterionic, nonionic surfactants and surfactants with similar structures based on traditional analytical techniques. We developed a high-throughput method for distinguishing various surfactants based on the adaptive emission profile as fingerprints (AEPF). The fluorescence response of the sensor was based on the interaction between surfactants and 1,3-diacetylpyrene (o-DAP) probe. The interaction affected the reversible conversion of free molecules and two aggregates in the solution, thereby changing the relative abundance and the fluorescence intensity ratio of two aggregates emitting different fluorescence. The o-DAP sensor can distinguish four types of surfactants (16 surfactants), especially surfactants of the same type with similar structures. The o-DAP sensor sensitively determined the critical micelle concentration (CMC) of 16 surfactants based on the interaction between o-DAP and surfactants. Additionally, the o-DAP sensor can detect and distinguish artificial vesicles made from different surfactants.
Zhai H., Wang Y., Guo Q., Zhang Y., Sun X., Guo Y., Zhang Y.
Talanta scimago Q1 wos Q1
2024-05-01 citations by CoLab: 6 Abstract  
The excessive content of lead (Pb(II)) and Staphylococcus aureus (S.aureus) seriously harms the quality of aquatic products. In this paper, a highly sensitive electrochemiluminescence (ECL) biosensor was constructed using the synergistic effect of Au NPs@Nickel-Cobalt-Metal-organic frameworks (Au@Ni-Co-MOFs) and double potential resolution function of urchin-like Au@luminol and Cadmium sulfide quantum dots (CdS QDs) for synchronous detection of Pb(II) and S.aureus in aquatic products. Au@Ni-Co-MOFs as the base material, its cube structure can improve the surface active area and sensitivity of the sensor, providing more catalytic active sites for the two functional probes. Urchin-like Au@luminol binding aptamer DNA2 specifically recognizes Pb(II), CdS QDs binding aptamer DNA3 specifically recognizes S.aureus, which collaboratively catalyzed hydrogen peroxide reduction to produce two electrochemiluminescence signals. The shared hairpin structure DNA1 binds stably to Au@Ni-Co-MOFs via the Au-S bond, and the two functional probes are complementary paired with the DNA1 respectively to ensure the specificity of the aptamer. According to the ECL intensity changes of different potentials signal sources, the synchronous detection of Pb(II) and S.aureus with different concentrations is realized. The sensor realizes the detection of two targets in aquatic products and provides a new strategy for the simultaneous detection of multiple targets.
Du L., Zhang H., Zhang F., Xia J., Meng Q., Huang H., Wang Z.
Talanta scimago Q1 wos Q1
2024-04-01 citations by CoLab: 12 Abstract  
Developing a highly selective and sensitive analysis strategy for lincomycin (LIN) is of great significance for environmental protection and food safety. Herein, we reported a novel electrochemiluminescence (ECL) aptasensor based on Ti3C2 QDs-1T/2H MoS2 nano-hybrid luminophore for detection of LIN. The hybridization of Ti3C2 QDs and 1T/2H MoS2 endowed nanocomposite with structural and compositional advantages for boosting the ECL performance of QDs by about three times. This enhancement could be attributed to the remarkable electrocatalytic activity and high conductivity exhibited by 1T/2H MoS2. Secondly, the great surface area of 1T/2H MoS2 is conducive to the high dispersion of Ti3C2 QDs, and its good conductivity could promote charge transfer. On the other hand, the excellent catalytic performance of 1T/2H MoS2 could facilitate the reduction of S2O82− to produce more radical, which significantly enhance the ECL signal of Ti3C2 QDs. Given these features, a sensor for detection of LIN was established based on specific recognition between target and aptamer. The sensor showed a good linear relationship (0.05 ng mL−1 ∼100 μg mL−1) with a detection limit as low as 0.02 ng mL−1. It is worth noting that this work has been validated in testing milk samples, exhibiting great potential application prospects in food analysis.
Liu L., Zou Y., Xia T., Zhang J., Xiong M., Long L., Wang K., Hao N.
Biosensors and Bioelectronics scimago Q1 wos Q1
2023-11-01 citations by CoLab: 9 Abstract  
The global spread of environmental biological pollutants, such as antibiotic-resistant bacteria and their antibiotic resistance genes (ARGs), has emerged as a critical public health concern. It is imperative to address this pressing issue due to its potential implications for public health. Herein, a DNA paperclip probe with double-quenching function of target cyclic cleavage was proposed, and an electrochemiluminescence (ECL) biosensing platform was constructed using Ti3C2 MXene in-situ reduction growth of Au NPs (TCM-Au) as a coreactant accelerator, and applied to the sensitive detection of ARGs. Thanks to the excellent catalytic performance, large surface area and Au–S affinity of TCM-Au, the ECL performance of CdS QDs have been significantly improved. By cleverly utilizing the negative charge of the paperclip nucleic acid probe and its modification group, double-quenching of the ECL signal was achieved. This innovative approach, combined with target cyclic amplification, facilitated specific and sensitive detection of the mecA gene. This biosensing platform manifested highly selective and sensitive determination of mecA genes in the range of 10 fM to 100 nM and a low detection limit of 2.7 fM. The credible detectability and anti-interference were demonstrated in Yangtze river and Aeration tank outlet, indicating its promising application toward pollution monitoring of ARGs.
Shelash Al-Hawary S.I., Malviya J., Althomali R.H., Almalki S.G., Kim K., Romero-Parra R.M., Fahad Ahmad A., Sanaan Jabbar H., Vaseem Akram S., Hussien Radie A.
2023-09-20 citations by CoLab: 3
Liu L., Peng M., Xu K., Xia H., Peng X., Peng L., Zhang J.Z.
Microchimica Acta scimago Q1 wos Q1
2023-09-11 citations by CoLab: 3 Abstract  
Molecularly imprinted polymers with methylammonium lead halide perovskite quantum dots (MIP@MAPbBr3 PQDs) have been prepared and applied to the determination of benzo(a)pyrene (BaP) for the first time. The photoluminescence (PL) of MIP@MAPbBr3 PQDs was enhanced due to the surface passivation of defects by BaP. PL excitation and emission spectra, X-ray diffraction, Fourier transform infrared, and time-resolved PL studies suggest that the interaction between MIP@MAPbBr3 PQDs and BaP is a dynamic process. After MIP@MAPbBr3 PQDs were incubated with BaP, the benzene ring in the molecular structure of BaP can interact with MIP@MAPbBr3 PQDs through π electrons, which reduces non-radiative recombination of MIP@MAPbBr3 PQDs and lengthens excited state lifetime. The PL intensity of the MIP@MAPbBr3 PQDs-BaP system was monitored at 520 nm with 375 nm excitation. Under optimized conditions, the PL intensity of MIP@MAPbBr3 PQDs is linear with the concentration of BaP in the 10 to 100 ng·mL−1 range, with a detection limit of 1.6 ng·mL−1. The imprinting factor was 3.9, indicating excellent specificity of MIP@MAPbBr3 PQDs for BaP. The MIP@MAPbBr3 PQDs were subsequently applied to the PL analysis of BaP in sunflower seed oil, cured meat, and grilled fish samples, achieving recoveries from 79.3 to 107%, and relative standard deviations below 10%. This molecularly imprinted fluorescence assay improves the selectivity of BaP in complex mixtures and could be extended to other analytes.
Yang L., Li J.
Chemosensors scimago Q2 wos Q1 Open Access
2023-08-04 citations by CoLab: 7 PDF Abstract  
Electrochemiluminescence (ECL) is a light-emitting process triggered by the high energy redox between electrochemically oxidized and reduced luminophores or some coreactive intermediate radicals, representing a blooming hot topic over decades with a wide variety of bioanalytical applications. Due to the superb sensitivity, ultralow background noise, specificity, ease of integration, and real-time and in situ analysis, ECL has been developed as a convenient and versatile technique for immunodiagnostics, nucleic acid analysis, and bioimaging. Discovering highly-efficient ECL emitters has been a promising subject that will benefit the development of sensitive bioanalytical methods with prominent potential prospects. To date, the interdisciplinary integrations of electrochemistry, spectroscopy, and nanoscience have brought up the continuous emergences of novel nanomaterials which can be flexibly conjugated with specific bio-recognition elements as functional ECL emitters for bioassays. Therefore, a critical overview of recent advances in developing highly-efficient ECL emitters for ultrasensitive detection of protein biomarkers is presented in this review, where six kinds of the most promising ECL nanomaterials for biosensing and imaging of various disease-related protein biomarkers are separately introduced with references to representative works. Finally, this review discusses the ongoing opportunities and challenges of ECL emitters in developing advanced bioassays for single-molecule analysis and spatiotemporally resolved imaging of protein biomarkers with future perspectives.
Althomali R.H., Hamoud Alshahrani S., Qasim almajidi Y., Kamal Hasan W., Gulnoza D., Romero-Parra R.M., Abid M.K., Radie Alawadi A.H., Alsalamyh A., Juyal A.
2023-07-22 citations by CoLab: 2
Li B., Wang Q., Sohail M., Zhang X., He H., Lin L.
Microchemical Journal scimago Q1 wos Q1
2023-06-01 citations by CoLab: 1 Abstract  
The blooming of cyanobacteria in aquatic environments can produce various cyanotoxins polluting soil and water, raising major concerns over environmental and human health issues. Microcystins (MCs) are one of the most toxic cyanotoxins, and the monitoring of MCs is critical for environmental protection and public health. Bio-inspired sensors, also known as biosensors, have become an emerging tool for the detection of MCs. Compared with conventional assays rely on centralized instruments and expensive reagents, biosensors are easier-to-use and more cost-effective, exhibiting high potential to be popularized in on-site and real-time MCs monitoring. Herein, the conventional methods for the MCs measurement were first briefly introduced, followed by the summarization of the recent trends in developing aptasensors and immunosensors targeting MCs, based on the recognition mechanisms towards MCs. Their common strategies and personalized highlights are discussed, to outline a general guide for designing MCs biosensors. Furthermore, the challenges in the biosensing of MCs regarding the specificity, sensitivity, simplicity, and stability are summarized to illustrate the future opportunities in this field.
Yarkaeva Y.A., Dymova D.A., Nazyrov M.I., Zagitova L.R., Maistrenko V.N.
Chimica Techno Acta scimago Q4 Open Access
2023-04-28 citations by CoLab: 0 Abstract  
For the selective detection of the antibiotic lincomycin, we developed a voltammetric sensor based on a glassy carbon electrode modified with reduced graphene oxide and polyarylenephthalide containing diphenylenethio and diphenyleneoxide fragments in the main chain of the polymer in the 1:1 ratio with lincomycin molecular imprints obtained by phase inversion. Using FTIR spectroscopy, electrochemical impedance spectroscopy, cyclic and differential-pulse voltammetry, the electrochemical and analytical characteristics of the sensor were studied. The detection of lincomycin was carried out by differential pulse voltammetry. The linear concentration range was 2.5·10–7–5·10–4 M with a limit of detection of 6.8·10–8 M. It was shown that the presence of molecular imprints increases the sensitivity of the developed sensor in comparisons with a sensor with non-imprinted polymer by a factor of 3.05.
Su C., Dong C., Jiang D., Shan X., Chen Z.
Microchemical Journal scimago Q1 wos Q1
2023-04-01 citations by CoLab: 10 Abstract  
Acetamiprid is a kind of broad-spectrum pesticides that has been largely applied in agriculture and household pest control. In this paper, a simple, novel and highly sensitive electrochemiluminescence aptamer sensor (ECL aptasensor) based on Ag3PO4-NH2-SnO2 was proposed. The ECL signal was greatly amplified with the synergistic effect of Ag3PO4 and NH2-SnO2 (Ag3PO4-NH2-SnO2) which was dropped on a glass carbon electrode (GCE) and used as working electrode. We designed an “on–off” ECL sensing strategy for the sensitive and specific detection of acetamiprid. Under the optimal conditions, the linear range of the sensor for acetamiprid detection was 1.0 × 10−11 ∼ 1.0 × 10−5 M, with a detection limit of 2.5 × 10−12 M (S/N = 3). The method showed simple and fast operation, high sensitivity and selectivity, strong anti-interference ability and good stability. More importantly, the developed aptasensor exhibited excellent recognition ability towards residual acetamiprid in actual water samples, which might open promising avenues to develop new ECL systems for biological analysis and environmental water monitoring.

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