Microchimica Acta, volume 192, issue 2, publication number 100

Smartphone-based non-invasive detection of salivary uric acid based on the fluorescence quenching of gleditsia sinensis carbon dots

Yujuan Xiang ¹

Hongpeng Yu ^{1, 2}

Kegang Wu ^{1, 2}

Ziting Quan ¹

Dong He ^{1, 2}

Cansheng Ou ¹

Xianghua Chai ¹

Yinglin Liang ¹

Xuejuan Duan ¹

Xiangyu Zhang ³

Qin Zha ³

Wei Xie ⁴

Show full list: 12 authors

Hide authors affiliations

School of Chemical Engineering and Light Industry, Guangdong University of Technology, Panyu District, Guangzhou, People’s Republic of China

Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center, Guangdong University of Technology, Jieyang City, People’s Republic of China

Institute of Traditional Chinese Medicine, Guizhou Province, Bijie City, China

⁴

Guizhou Province, Qianzhi Mingguang Soaphorn Rice Processing Base, Zhijin County, Bijie CityBijie City, China

Publication type: Journal Article

Publication date: 2025-01-21

Springer Nature

Journal: Microchimica Acta

scimago Q1

SJR: 0.848

CiteScore: 9.8

Impact factor: 5.3

ISSN: 00263672, 14365073

DOI: 10.1007/s00604-025-06949-w

Copy DOI

Abstract

A smartphone-based non-invasive method was developed for salivary uric acid detection using Gleditsia Sinensis carbon dots (GS-CDs). The GS-CDs synthesized by the one-pot hydrothermal method emitted blue fluorescence at a maximum excitation wavelength of 350 nm and had good fluorescence stability in the presence of different ions, while showing selectivity to uric acid solution. The ability of uric acid (UA) to quench the fluorescent substances present in the GS-CDs, was confirmed through HPLC-FLD and LC–MS, FTIR and XPS. The results showed that UA reacted with GS-CDs, with a decrease in hydroxyl groups and the formation of carboxyl groups. The fluorescence quenching suggested a possible dynamic quenching mechanism. In addition, a smartphone-based non-invasive detection method was developed for the detection of salivary UA levels, which reflects blood UA levels. This study provides a new perspective on the utilization of GS shells and advances the development of non-invasive testing for UA.