volume 33 issue 2 pages 109-118

Densitometric simultaneous estimation of combination of empagliflozin, linagliptin and metformin hydrochloride used in the treatment of type 2 diabetes mellitus

Ishita M Patel 1
Usmangani K Chhalotiya 1
Harsha D Jani 1
Devansh Kansara 1
Dimal A Shah 2
1
 
Department of Pharmaceutical Chemistry and Analysis, Indukaka Ipcowala College of Pharmacy, Vitthal Udyognagar, India
2
 
Babaria Institute of Pharmacy, Vadodara, India
Publication typeJournal Article
Publication date2020-04-01
scimago Q3
wos Q4
SJR0.250
CiteScore1.9
Impact factor1.1
ISSN09334173, 17890993
Biochemistry
Clinical Biochemistry
Analytical Chemistry
Abstract
A sensitive, accurate, precise, and robust high-performance thin-layer chromatography (HPTLC) method has been developed for the simultaneous quantification of empagliflozin, linagliptin, and metformin HCl in bulk and synthetic mixture. The method applied HPTLC aluminum plates pre-coated with silica gel 60 F254 as the stationary phase along with n-butanol:water:glacial acetic acid (6:3:1, v/v) as the mobile phase. The Rf values of empagliflozin, linagliptin, and metformin HCl were found to be 0.73, 0.52, and 0.33, respectively. Densitometric analysis was carried out in the absorbance mode at 223 nm. The method was obtained linear in the range of 0.1–0.7 μg/band for empagliflozin, 0.05–0.35 μg/band for linagliptin, and 10–70 μg/band for metformin HCl, respectively. From synthetic mixture, the percentage (%) amounts of all three drugs were found to be more than 98% by the proposed HPTLC method. The method was validated as per the International Conference on Harmonisation (ICH) guideline Q2(R1).
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Patel I. M. et al. Densitometric simultaneous estimation of combination of empagliflozin, linagliptin and metformin hydrochloride used in the treatment of type 2 diabetes mellitus // Journal of Planar Chromatography - Modern TLC. 2020. Vol. 33. No. 2. pp. 109-118.
GOST all authors (up to 50) Copy
Patel I. M., Chhalotiya U. K., Jani H. D., Kansara D., Shah D. A. Densitometric simultaneous estimation of combination of empagliflozin, linagliptin and metformin hydrochloride used in the treatment of type 2 diabetes mellitus // Journal of Planar Chromatography - Modern TLC. 2020. Vol. 33. No. 2. pp. 109-118.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1007/s00764-020-00026-z
UR - https://doi.org/10.1007/s00764-020-00026-z
TI - Densitometric simultaneous estimation of combination of empagliflozin, linagliptin and metformin hydrochloride used in the treatment of type 2 diabetes mellitus
T2 - Journal of Planar Chromatography - Modern TLC
AU - Patel, Ishita M
AU - Chhalotiya, Usmangani K
AU - Jani, Harsha D
AU - Kansara, Devansh
AU - Shah, Dimal A
PY - 2020
DA - 2020/04/01
PB - Akademiai Kiado
SP - 109-118
IS - 2
VL - 33
SN - 0933-4173
SN - 1789-0993
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2020_Patel,
author = {Ishita M Patel and Usmangani K Chhalotiya and Harsha D Jani and Devansh Kansara and Dimal A Shah},
title = {Densitometric simultaneous estimation of combination of empagliflozin, linagliptin and metformin hydrochloride used in the treatment of type 2 diabetes mellitus},
journal = {Journal of Planar Chromatography - Modern TLC},
year = {2020},
volume = {33},
publisher = {Akademiai Kiado},
month = {apr},
url = {https://doi.org/10.1007/s00764-020-00026-z},
number = {2},
pages = {109--118},
doi = {10.1007/s00764-020-00026-z}
}
MLA
Cite this
MLA Copy
Patel, Ishita M., et al. “Densitometric simultaneous estimation of combination of empagliflozin, linagliptin and metformin hydrochloride used in the treatment of type 2 diabetes mellitus.” Journal of Planar Chromatography - Modern TLC, vol. 33, no. 2, Apr. 2020, pp. 109-118. https://doi.org/10.1007/s00764-020-00026-z.