A validated high-performance thin-layer chromatography method for quantification of bavachin, bakuchiol, and psoralen from Psoralea corylifolia seeds

A sensitive and reliable high-performance thin-layer chromatography method has been developed for the simultaneous estimation of bavachin, bakuchiol, and psoralen in Psoralea corylifolia seeds. The methanolic extract of the seeds was applied on a silica gel G 60 F254 plate. The plate was developed using toluene–ether, 1:1, saturated with 10% acetic acid (v/v) as the mobile phase and detection and quantification were performed by densitometric scanning at 254 nm. The correlation coefficients were found to be 0.999, 0.999, and 0.999 for bavachin, bakuchiol, and psoralen, respectively, in the developed method. The accuracy of the method was confirmed by conducting recovery studies at different levels using the standard addition method. The average recovery of bavachin, bakuchiol, and psoralen was found to be close to 98% confirming the accurateness of the method. The proposed method offers a new, sensitive, specific, and precise gauge for quantification of bavachin, bakuchiol, and psoralen representing all the categories of bioactive constituents in P. corylifolia.