Springer Nature
Infection
,  volume 52 ,  issue 4

Portable smartphone-based molecular test for rapid detection of Leishmania spp.

Rea Maja Kobialka 1
, 
Arianna Ceruti 1
, 
Madhurima Roy 2
, 
Sutopa Roy 2
, 
Rajashree Chowdhury 3
, 
Prakash Ghosh 3
, 
Faria Hossain 3
, 
Manfred Weidmann 4
, 
Elena Graf 4
, 
Jesus Bueno Alvarez 4
, 
Javier Moreno 5
, 
Uwe Truyen 1
, 
Dinesh Mondal 3
, 
Mitali Chatterjee 2
, 
Ahmed Abd El Wahed 1
1
 
2
 
3
 
Nutrition Research Division, International Centre for Diarrheal Disease Research Bangladesh, Dhaka, Bangladesh | 
4
 
Midge Medical GmbH, Berlin, Germany | 
5
 
Publication type: Journal Article
Publication date: 2024-02-14
Springer Nature
scimago Q1
wos Q2
SJR: 1.167
CiteScore: 10.4
Impact factor: 3.6
ISSN: 03008126, 14390973
General Medicine
Microbiology (medical)
Infectious Diseases
Abstract
Purpose

Leishmaniasis, caused by the parasite of the genus Leishmania, is a neglected tropical disease which is endemic in more than 60 countries. In South-East Asia, Brazil, and East Africa, it mainly occurs as kala-azar (visceral leishmaniasis, VL), and subsequently as post kala-azar dermal leishmaniasis (PKDL) in a smaller portion of cases. As stated per WHO roadmap, accessibility to accurate diagnostic methods is an essential step to achieve elimination. This study aimed to test the accuracy of a portable minoo device, a small battery-driven, multi-use fluorimeter operating with isothermal technology for molecular diagnosis of VL and PKDL.

Methods

Fluorescence data measured by the device within 20 min are reported back to the mobile application (or app) via Bluetooth and onward via the internet to a backend. This allows anonymous analysis and storage of the test data. The test result is immediately returned to the app displaying it to the user.

Results

The limit of detection was 11.2 genome copies (95% CI) as determined by screening a tenfold dilution range of whole Leishmania donovani genomes using isothermal recombinase polymerase amplification (RPA). Pathogens considered for differential diagnosis were tested and no cross-reactivity was observed. For its diagnostic performance, DNA extracted from 170 VL and PKDL cases, comprising peripheral blood samples (VL, n = 96) and skin biopsies (PKDL, n = 74) from India (n = 108) and Bangladesh (n = 62), was screened. Clinical sensitivity and specificity were 88% and 91%, respectively.

Conclusion

Minoo devices can offer a convenient, cheaper alternative to other molecular diagnostics. Its easy handling makes it ideal for use in low-resource settings to identify parasite burden.

Found 
Found 

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GOST Copy
Kobialka R. M. et al. Portable smartphone-based molecular test for rapid detection of Leishmania spp. // Infection. 2024. Vol. 52. No. 4.
GOST all authors (up to 50) Copy
Kobialka R. M., Ceruti A., Roy M., Roy S., Chowdhury R., Ghosh P., Hossain F., Weidmann M., Graf E., Bueno Alvarez J., Moreno J., Truyen U., Mondal D., Chatterjee M., Abd El Wahed A. Portable smartphone-based molecular test for rapid detection of Leishmania spp. // Infection. 2024. Vol. 52. No. 4.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1007/s15010-024-02179-z
UR - https://doi.org/10.1007/s15010-024-02179-z
TI - Portable smartphone-based molecular test for rapid detection of Leishmania spp.
T2 - Infection
AU - Kobialka, Rea Maja
AU - Ceruti, Arianna
AU - Roy, Madhurima
AU - Roy, Sutopa
AU - Chowdhury, Rajashree
AU - Ghosh, Prakash
AU - Hossain, Faria
AU - Weidmann, Manfred
AU - Graf, Elena
AU - Bueno Alvarez, Jesus
AU - Moreno, Javier
AU - Truyen, Uwe
AU - Mondal, Dinesh
AU - Chatterjee, Mitali
AU - Abd El Wahed, Ahmed
PY - 2024
DA - 2024/02/14
PB - Springer Nature
IS - 4
VL - 52
PMID - 38353873
SN - 0300-8126
SN - 1439-0973
ER -
BibTex
Cite this
BibTex (up to 50 authors) Copy
@article{2024_Kobialka,
author = {Rea Maja Kobialka and Arianna Ceruti and Madhurima Roy and Sutopa Roy and Rajashree Chowdhury and Prakash Ghosh and Faria Hossain and Manfred Weidmann and Elena Graf and Jesus Bueno Alvarez and Javier Moreno and Uwe Truyen and Dinesh Mondal and Mitali Chatterjee and Ahmed Abd El Wahed},
title = {Portable smartphone-based molecular test for rapid detection of Leishmania spp.},
journal = {Infection},
year = {2024},
volume = {52},
publisher = {Springer Nature},
month = {feb},
url = {https://doi.org/10.1007/s15010-024-02179-z},
number = {4},
doi = {10.1007/s15010-024-02179-z}
}