Stable plastid transformation in kiwifruit (Actinidia chinensis)

Publication type: Journal Article
Publication date: 2024-11-28
Springer Nature
scimago Q1
wos Q1
SJR: 1.257
CiteScore: 8.2
Impact factor: 5.0
ISSN: 26621738, 20966326
Abstract

Plastid transformation offers valuable benefits in plant biotechnology, such as high-level transgene expression and the absence of gene silencing. Here we describe the first protocol of a plastid transformation system for a woody vine (liana) kiwifruit (Actinidia chinensis). The transgenic DNA carries a spectinomycin-resistance gene (aadA) cassette and a green fluorescent protein (GFP) reporter gene cassette, flanked by two adjacent kiwifruit plastid genome sequences, thereby allowing targeted insertion between the trnfM and trnG genes. Six spectinomycin-resistant shoots were obtained out of 12 plates subjected to bombardment, and two were positive events, confirmed through PCR and Southern blot analyses. The GFP was localized to plastids as monitored by confocal laser scanning microscopy and reached 2.5% of leaf total soluble protein. Success in kiwifruit extends transplastomic technology of woody species beyond poplar, and will provide an attractive biosynthetic chassis for molecular farming.

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GOST Copy
Chen Q. et al. Stable plastid transformation in kiwifruit (Actinidia chinensis) // aBIOTECH. 2024.
GOST all authors (up to 50) Copy
Chen Q., Wu Y., Wang Y., Zhang J., Li S. Stable plastid transformation in kiwifruit (Actinidia chinensis) // aBIOTECH. 2024.
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RIS Copy
TY - JOUR
DO - 10.1007/s42994-024-00186-0
UR - https://link.springer.com/10.1007/s42994-024-00186-0
TI - Stable plastid transformation in kiwifruit (Actinidia chinensis)
T2 - aBIOTECH
AU - Chen, Qiqi
AU - Wu, Yuyong
AU - Wang, Yanchang
AU - Zhang, Jiang
AU - Li, Shengchun
PY - 2024
DA - 2024/11/28
PB - Springer Nature
SN - 2662-1738
SN - 2096-6326
ER -
BibTex
Cite this
BibTex (up to 50 authors) Copy
@article{2024_Chen,
author = {Qiqi Chen and Yuyong Wu and Yanchang Wang and Jiang Zhang and Shengchun Li},
title = {Stable plastid transformation in kiwifruit (Actinidia chinensis)},
journal = {aBIOTECH},
year = {2024},
publisher = {Springer Nature},
month = {nov},
url = {https://link.springer.com/10.1007/s42994-024-00186-0},
doi = {10.1007/s42994-024-00186-0}
}