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Springer Nature
Methods in Molecular Biology
,  том 1720 ,  страницы 187-204

Generation of cell lines stably expressing a fluorescent reporter of nonsense-mediated mRNA decay activity

Тип публикации: Book Chapter
Дата публикации: 2017-12-14
Springer Nature
scimago Q4
БС3
SJR: 0.354
CiteScore: 2.1
Impact factor: —
ISSN: 10643745, 19406029
Краткое описание
Nonsense-mediated mRNA decay (NMD) is a mechanism of mRNA surveillance ubiquitous among eukaryotes. Importantly, NMD not only removes aberrant transcripts with premature stop codons, but also regulates expression of many normal genes. A recently introduced dual-color fluorescent protein-based reporter enables analysis of NMD activity in live cells. In this chapter we describe the method to generate stable transgenic cell lines expressing the splicing-dependent NMD reporter using consecutive steps of lentivirus transduction and Tol2 transposition.
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Markina N. M. et al. Generation of cell lines stably expressing a fluorescent reporter of nonsense-mediated mRNA decay activity // Methods in Molecular Biology. 2017. Vol. 1720. pp. 187-204.
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Markina N. M., Pereverzev A. P., Staroverov D. B., Lukyanov K. A., GURSKAYA N. G. Generation of cell lines stably expressing a fluorescent reporter of nonsense-mediated mRNA decay activity // Methods in Molecular Biology. 2017. Vol. 1720. pp. 187-204.
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TY - GENERIC
DO - 10.1007/978-1-4939-7540-2_14
UR - https://doi.org/10.1007/978-1-4939-7540-2_14
TI - Generation of cell lines stably expressing a fluorescent reporter of nonsense-mediated mRNA decay activity
T2 - Methods in Molecular Biology
AU - Markina, Nadezhda M
AU - Pereverzev, Anton P
AU - Staroverov, Dmitry B.
AU - Lukyanov, Konstantin A.
AU - GURSKAYA, Nadya G.
PY - 2017
DA - 2017/12/14
PB - Springer Nature
SP - 187-204
VL - 1720
PMID - 29236260
SN - 1064-3745
SN - 1940-6029
ER -
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@incollection{2017_Markina,
author = {Nadezhda M Markina and Anton P Pereverzev and Dmitry B. Staroverov and Konstantin A. Lukyanov and Nadya G. GURSKAYA},
title = {Generation of cell lines stably expressing a fluorescent reporter of nonsense-mediated mRNA decay activity},
publisher = {Springer Nature},
year = {2017},
volume = {1720},
pages = {187--204},
month = {dec}
}