Open Access

Methods in Molecular Biology, pages 261-272

The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies

Marion Marie-Jeanne ^{1, 2}

Hantz Olivier ^{1, 2}

Durantel David ^{1, 2, 3}

Hide authors affiliations Show authors affiliations: 3 affiliations

INSERM U871, Molecular physiopathology and new treatments of viral hepatitis, Lyon, France |

Université de Lyon, and IFR62, Lyon, France |

Hospices Civils de Lyon (HCL), Lyon, France |

Publication type: Book Chapter

Publication date: 2010-07-19

Springer Nature

Journal: Methods in Molecular Biology

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Impact factor: —

ISSN: 10643745, 19406029

DOI: 10.1007/978-1-60761-688-7_13

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Abstract

Liver progenitor cells may play an important role in carcinogenesis in vivo and represent therefore useful cellular materials for in vitro studies. The HepaRG cell line, which is a human bipotent progenitor cell line capable to differentiate toward two different cell phenotypes (i.e., biliary-like and hepatocyte-like cells), has been established from a liver tumor associated with chronic hepatitis C. This cell line represents a valuable alternative to ex vivo cultivated primary human hepatocytes (PHH), as HepaRG cells share some features and properties with adult hepatocytes. The cell line is particularly useful to evaluate drugs and perform drug metabolism studies, as many detoxifying enzymes are expressed and functional. It is also an interesting tool to study some aspect of progenitor biology (e.g., differentiation process), carcinogenesis, and the infection by some pathogens for which the cell line is permissive (e.g., HBV infection). Overall, this chapter gives a concise overview of the biological properties and potential applications of this cell line.

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Citations by journals

	1 2 3
Archives of Toxicology	Archives of Toxicology, 3, 3.3% Archives of Toxicology 3 publications, 3.3%
PLoS ONE	PLoS ONE, 3, 3.3% PLoS ONE 3 publications, 3.3%
Journal of Hepatology	Journal of Hepatology, 3, 3.3% Journal of Hepatology 3 publications, 3.3%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 2, 2.2% International Journal of Molecular Sciences 2 publications, 2.2%
Scientific Reports	Scientific Reports, 2, 2.2% Scientific Reports 2 publications, 2.2%
ACS Omega	ACS Omega, 2, 2.2% ACS Omega 2 publications, 2.2%
Journal of Biological Chemistry	Journal of Biological Chemistry, 2, 2.2% Journal of Biological Chemistry 2 publications, 2.2%
Gut	Gut, 2, 2.2% Gut 2 publications, 2.2%
Antimicrobial Agents and Chemotherapy	Antimicrobial Agents and Chemotherapy, 2, 2.2% Antimicrobial Agents and Chemotherapy 2 publications, 2.2%
Journal of Virology	Journal of Virology, 2, 2.2% Journal of Virology 2 publications, 2.2%
Doklady Biochemistry and Biophysics	Doklady Biochemistry and Biophysics, 1, 1.1% Doklady Biochemistry and Biophysics 1 publication, 1.1%
Journal of Immunology	Journal of Immunology, 1, 1.1% Journal of Immunology 1 publication, 1.1%
APL Photonics	APL Photonics, 1, 1.1% APL Photonics 1 publication, 1.1%
Drug Metabolism and Disposition	Drug Metabolism and Disposition, 1, 1.1% Drug Metabolism and Disposition 1 publication, 1.1%
Cellular Reprogramming	Cellular Reprogramming, 1, 1.1% Cellular Reprogramming 1 publication, 1.1%
Journal of General Virology	Journal of General Virology, 1, 1.1% Journal of General Virology 1 publication, 1.1%
Cells	Cells, 1, 1.1% Cells 1 publication, 1.1%
Hepatology	Hepatology, 1, 1.1% Hepatology 1 publication, 1.1%
Transplantation	Transplantation, 1, 1.1% Transplantation 1 publication, 1.1%
Cancers	Cancers, 1, 1.1% Cancers 1 publication, 1.1%
Nanobiomedicine	Nanobiomedicine, 1, 1.1% Nanobiomedicine 1 publication, 1.1%
Antiviral Therapy	Antiviral Therapy, 1, 1.1% Antiviral Therapy 1 publication, 1.1%
Viruses	Viruses, 1, 1.1% Viruses 1 publication, 1.1%
Biology	Biology, 1, 1.1% Biology 1 publication, 1.1%
Veterinary Research	Veterinary Research, 1, 1.1% Veterinary Research 1 publication, 1.1%
Virology Journal	Virology Journal, 1, 1.1% Virology Journal 1 publication, 1.1%
Hereditas	Hereditas, 1, 1.1% Hereditas 1 publication, 1.1%
Epigenetics and Chromatin	Epigenetics and Chromatin, 1, 1.1% Epigenetics and Chromatin 1 publication, 1.1%
PLoS Pathogens	PLoS Pathogens, 1, 1.1% PLoS Pathogens 1 publication, 1.1%
	1 2 3

Citations by publishers

	2 4 6 8 10 12 14 16 18
Elsevier	Elsevier, 17, 18.68% Elsevier 17 publications, 18.68%
Springer Nature	Springer Nature, 11, 12.09% Springer Nature 11 publications, 12.09%
Wiley	Wiley, 8, 8.79% Wiley 8 publications, 8.79%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 7, 7.69% Multidisciplinary Digital Publishing Institute (MDPI) 7 publications, 7.69%
American Society for Microbiology	American Society for Microbiology, 7, 7.69% American Society for Microbiology 7 publications, 7.69%
Public Library of Science (PLoS)	Public Library of Science (PLoS), 4, 4.4% Public Library of Science (PLoS) 4 publications, 4.4%
American Chemical Society (ACS)	American Chemical Society (ACS), 4, 4.4% American Chemical Society (ACS) 4 publications, 4.4%
SAGE	SAGE, 2, 2.2% SAGE 2 publications, 2.2%
American Society for Biochemistry and Molecular Biology	American Society for Biochemistry and Molecular Biology, 2, 2.2% American Society for Biochemistry and Molecular Biology 2 publications, 2.2%
Frontiers Media S.A.	Frontiers Media S.A., 2, 2.2% Frontiers Media S.A. 2 publications, 2.2%
Taylor & Francis	Taylor & Francis, 2, 2.2% Taylor & Francis 2 publications, 2.2%
BMJ	BMJ, 2, 2.2% BMJ 2 publications, 2.2%
Pleiades Publishing	Pleiades Publishing, 1, 1.1% Pleiades Publishing 1 publication, 1.1%
The American Association of Immunologists	The American Association of Immunologists, 1, 1.1% The American Association of Immunologists 1 publication, 1.1%
American Institute of Physics (AIP)	American Institute of Physics (AIP), 1, 1.1% American Institute of Physics (AIP) 1 publication, 1.1%
American Society for Pharmacology and Experimental Therapeutics	American Society for Pharmacology and Experimental Therapeutics, 1, 1.1% American Society for Pharmacology and Experimental Therapeutics 1 publication, 1.1%
Mary Ann Liebert	Mary Ann Liebert, 1, 1.1% Mary Ann Liebert 1 publication, 1.1%
Microbiology Society	Microbiology Society, 1, 1.1% Microbiology Society 1 publication, 1.1%
Wolters Kluwer Health	Wolters Kluwer Health, 1, 1.1% Wolters Kluwer Health 1 publication, 1.1%
King Saud University	King Saud University, 1, 1.1% King Saud University 1 publication, 1.1%
Royal Society of Chemistry (RSC)	Royal Society of Chemistry (RSC), 1, 1.1% Royal Society of Chemistry (RSC) 1 publication, 1.1%
American Association for Cancer Research (AACR)	American Association for Cancer Research (AACR), 1, 1.1% American Association for Cancer Research (AACR) 1 publication, 1.1%
American Physiological Society	American Physiological Society, 1, 1.1% American Physiological Society 1 publication, 1.1%
Annual Reviews	Annual Reviews, 1, 1.1% Annual Reviews 1 publication, 1.1%
AME Publishing Company	AME Publishing Company, 1, 1.1% AME Publishing Company 1 publication, 1.1%
	2 4 6 8 10 12 14 16 18

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Marion M. et al. The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies // Methods in Molecular Biology. 2010. pp. 261-272.

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Marion M., Hantz O., Durantel D. The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies // Methods in Molecular Biology. 2010. pp. 261-272.

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RIS Copy

TY - GENERIC

DO - 10.1007/978-1-60761-688-7_13

UR - https://doi.org/10.1007%2F978-1-60761-688-7_13

TI - The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies

T2 - Methods in Molecular Biology

AU - Marion, Marie-Jeanne

AU - Hantz, Olivier

AU - Durantel, David

PY - 2010

DA - 2010/07/19 00:00:00

PB - Springer Nature

SP - 261-272

SN - 1064-3745

SN - 1940-6029

ER -

BibTex

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BibTex Copy

@incollection{2010_Marion,

author = {Marie-Jeanne Marion and Olivier Hantz and David Durantel},

title = {The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies},

publisher = {Springer Nature},

year = {2010},

pages = {261--272},

month = {jul}

}

Found error?

Publisher

Springer Nature

Journal

Methods in Molecular Biology

Quartile SCImago

Quartile WOS

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Impact factor

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ISSN

10643745 (Print)
19406029 (Electronic)