Open Access
Methods in Molecular Biology, pages 219-236
Cellular Chemosensitivity Assays: An Overview
1
Department of Biotechnology, Indian Institute of Technology (IIT)-Chennai, 201, Bhupat & Jyothi Mehta School of Biosciences Chennai, Chennai, India
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Publication type: Book Chapter
Publication date: 2011-04-20
Journal:
Methods in Molecular Biology
Quartile SCImago
Q3
Quartile WOS
—
Impact factor: —
ISSN: 10643745, 19406029
Abstract
Data on cell viability have long been obtained from in vitro cytotoxicity assays. Today, there is a focus on markers of cell death, and the MTT cell survival assay is widely used for measuring cytotoxic potential of a compound. However, a comprehensive evaluation of cytotoxicity requires additional assays which -measure short and long-term cytotoxicity. Assays which measure the cytostatic effects of compounds are not less important, particularly for newer anticancer agents. This overview discusses the advantages and disadvantages of different non-clonogenic assays for measuring short and medium-term cytotoxicity. It also discusses clonogenic assays, which accurately measure long-term cytostatic effects of drugs and toxic agents. For certain compounds and cell types, the advent of high throughput, multiparameter, cytotoxicity assays, and gene expression assays have made it possible to predict cytotoxic potency in vivo.
Citations by journals
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Citations by publishers
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Taylor & Francis
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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TY - GENERIC
DO - 10.1007/978-1-61779-080-5_19
UR - https://doi.org/10.1007%2F978-1-61779-080-5_19
TI - Cellular Chemosensitivity Assays: An Overview
T2 - Methods in Molecular Biology
AU - Sumantran, Venil N.
PY - 2011
DA - 2011/04/20 00:00:00
PB - Springer Nature
SP - 219-236
SN - 1064-3745
SN - 1940-6029
ER -
Cite this
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@incollection{2011_Sumantran,
author = {Venil N. Sumantran},
title = {Cellular Chemosensitivity Assays: An Overview},
publisher = {Springer Nature},
year = {2011},
pages = {219--236},
month = {apr}
}