Die Naturwissenschaften, volume 98, issue 1, pages 7-13
Isolation and fractionation of soil humin using alkaline urea and dimethylsulphoxide plus sulphuric acid
1
Department of Chemical and Environmental Sciences, University of Limerick, Limerick, Ireland
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2
School of Sustainable Engineering and The Built Environment, Arizona State University, Tempe, USA
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3
EMBRAPA Solos, Rio de Janeiro, Brazil
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4
Department of Chemistry, Scarborough College, University of Toronto, Toronto, Canada
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Publication type: Journal Article
Publication date: 2010-11-23
Journal:
Die Naturwissenschaften
Quartile SCImago
Q2
Quartile WOS
Q3
Impact factor: 1.8
ISSN: 00281042, 14321904
General Medicine
Ecology, Evolution, Behavior and Systematics
Abstract
Humin, the most recalcitrant and abundant organic fraction of soils and of sediments, is a significant contributor to the stable carbon pool in soils and is important for the global carbon budget. It has significant resistance to transformations by microorganisms. Based on the classical operational definition, humin can include any humic-type substance that is not soluble in water at any pH. We demonstrate in this study how sequential exhaustive extractions with 0.1 M sodium hydroxide (NaOH) + 6 M urea, followed by dimethylsulphoxide (DMSO) + 6% (v/v) sulphuric acid (H2SO4) solvent systems, can extract 70–80% of the residual materials remaining after prior exhaustive extractions in neutral and aqueous basic media. Solid-state 13C NMR spectra have shown that the components isolated in the base + urea system were compositionally similar to the humic and fulvic acid fractions isolated at pH 12.6 in the aqueous media. The NMR spectra indicated that the major components isolated in the DMSO + H2SO4 medium had aliphatic hydrocarbon associated with carboxyl functionalities and with lesser amounts of carbohydrate and peptide and minor amounts of lignin-derived components. The major components will have significant contributions from long-chain fatty acids, waxes, to cuticular materials. The isolates in the DMSO + H2SO4 medium were compositionally similar to the organic components that resisted solvation and remained associated with the soil clays. It is concluded that the base + urea system released humic and fulvic acids held by hydrogen bonding or by entrapment within the humin matrix. The recalcitrant humin materials extracted in DMSO + H2SO4 are largely biological molecules (from plants and the soil microbial population) that are likely to be protected from degradation by their hydrophobic moieties and by sorption on the soil clays. Thus, the major components of humin do not satisfy the classical definitions for humic substances which emphasise that these arise from microbial or chemical transformations in soils of the components of organic debris.
Citations by journals
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6
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Science of the Total Environment
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Science of the Total Environment
6 publications, 12%
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Journal of Soils and Sediments
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Journal of Soils and Sediments
3 publications, 6%
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Organic Geochemistry
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Revista Brasileira de Ciencia do Solo
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Revista Brasileira de Ciencia do Solo
3 publications, 6%
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Advances in Agronomy
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Advances in Agronomy
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Journal of Material Cycles and Waste Management
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Journal of Material Cycles and Waste Management
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Journal of Geochemical Exploration
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Journal of Geochemical Exploration
2 publications, 4%
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Soil Biology and Biochemistry
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Soil Biology and Biochemistry
2 publications, 4%
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Journal of Agricultural and Food Chemistry
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Journal of Agricultural and Food Chemistry
2 publications, 4%
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Eurasian Soil Science
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Eurasian Soil Science
2 publications, 4%
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Pharmaceutical Chemistry Journal
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Pharmaceutical Chemistry Journal
1 publication, 2%
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International Journal of Environmental Research and Public Health
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International Journal of Environmental Research and Public Health
1 publication, 2%
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Agronomy
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Agronomy
1 publication, 2%
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Molecules
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1 publication, 2%
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Die Naturwissenschaften
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Die Naturwissenschaften
1 publication, 2%
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Environmental Science and Pollution Research
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Environmental Science and Pollution Research
1 publication, 2%
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Biology and Fertility of Soils
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Biology and Fertility of Soils
1 publication, 2%
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Chemical and Biological Technologies in Agriculture
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Chemical and Biological Technologies in Agriculture
1 publication, 2%
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Environmental Technology and Innovation
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Environmental Technology and Innovation
1 publication, 2%
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Chemical Engineering Journal
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Chemical Engineering Journal
1 publication, 2%
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Environmental Pollution
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Environmental Pollution
1 publication, 2%
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Chemosphere
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Chemosphere
1 publication, 2%
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Journal of Cleaner Production
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Journal of Cleaner Production
1 publication, 2%
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Soil and Tillage Research
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Soil and Tillage Research
1 publication, 2%
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Journal of Environmental Quality
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Journal of Environmental Quality
1 publication, 2%
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Soil and Water Research
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Soil and Water Research, 1, 2%
Soil and Water Research
1 publication, 2%
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Plant, Soil and Environment
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Plant, Soil and Environment, 1, 2%
Plant, Soil and Environment
1 publication, 2%
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Land Degradation and Development
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Land Degradation and Development
1 publication, 2%
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Applied Geochemistry
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Applied Geochemistry
1 publication, 2%
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Citations by publishers
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5
10
15
20
25
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Elsevier
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Elsevier
23 publications, 46%
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Springer Nature
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Springer Nature
10 publications, 20%
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Multidisciplinary Digital Publishing Institute (MDPI)
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Multidisciplinary Digital Publishing Institute (MDPI)
3 publications, 6%
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SciELO
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SciELO
3 publications, 6%
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American Chemical Society (ACS)
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American Chemical Society (ACS)
2 publications, 4%
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Pleiades Publishing
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Pleiades Publishing
2 publications, 4%
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Czech Academy of Agricultural Sciences
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Czech Academy of Agricultural Sciences, 2, 4%
Czech Academy of Agricultural Sciences
2 publications, 4%
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American Society of Agronomy
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American Society of Agronomy
1 publication, 2%
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Wiley
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Wiley
1 publication, 2%
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5
10
15
20
25
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- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Song G. et al. Isolation and fractionation of soil humin using alkaline urea and dimethylsulphoxide plus sulphuric acid // Die Naturwissenschaften. 2010. Vol. 98. No. 1. pp. 7-13.
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Song G., Hayes M. H. B., Novotny E. H., Simpson A. J. Isolation and fractionation of soil humin using alkaline urea and dimethylsulphoxide plus sulphuric acid // Die Naturwissenschaften. 2010. Vol. 98. No. 1. pp. 7-13.
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TY - JOUR
DO - 10.1007/s00114-010-0733-4
UR - https://doi.org/10.1007%2Fs00114-010-0733-4
TI - Isolation and fractionation of soil humin using alkaline urea and dimethylsulphoxide plus sulphuric acid
T2 - Die Naturwissenschaften
AU - Song, Guixue
AU - Hayes, Michael H B
AU - Novotny, Etelvino H.
AU - Simpson, André J
PY - 2010
DA - 2010/11/23 00:00:00
PB - Springer Nature
SP - 7-13
IS - 1
VL - 98
SN - 0028-1042
SN - 1432-1904
ER -
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@article{2010_Song,
author = {Guixue Song and Michael H B Hayes and Etelvino H. Novotny and André J Simpson},
title = {Isolation and fractionation of soil humin using alkaline urea and dimethylsulphoxide plus sulphuric acid},
journal = {Die Naturwissenschaften},
year = {2010},
volume = {98},
publisher = {Springer Nature},
month = {nov},
url = {https://doi.org/10.1007%2Fs00114-010-0733-4},
number = {1},
pages = {7--13},
doi = {10.1007/s00114-010-0733-4}
}
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MLA
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Song, Guixue, et al. “Isolation and fractionation of soil humin using alkaline urea and dimethylsulphoxide plus sulphuric acid.” Die Naturwissenschaften, vol. 98, no. 1, Nov. 2010, pp. 7-13. https://doi.org/10.1007%2Fs00114-010-0733-4.