Analytical and Bioanalytical Chemistry, volume 411, issue 19, pages 4899-4908
Selective single-molecule analytical detection of C-reactive protein in saliva with an organic transistor
Eleonora Macchia
1
,
Kyriaki Manoli
1
,
Brigitte Holzer
1
,
Cinzia Di Franco
2
,
Rosaria A Picca
1
,
Nicola Cioffi
1
,
Gaetano Scamarcio
3
,
Gerardo Palazzo
1, 4
,
Luisa Torsi
1, 4, 5
4
CSGI (Centre for Colloid and Surface Science), Bari, Italy
|
Publication type: Journal Article
Publication date: 2019-03-28
Quartile SCImago
Q2
Quartile WOS
Q1
Impact factor: 4.3
ISSN: 16182642, 16182650
PubMed ID:
30923859
Biochemistry
Analytical Chemistry
Abstract
In the last decade, saliva has been suggested as non-invasive diagnostic fluid, suitable for clinical use alternatively to blood serum and plasma. However, the clinical applicability of saliva has been hampered so far by the inadequate sensitivity of current methods to detect the lower salivary concentrations of many biomarkers monitored in blood products. Herein, a label-free biosensor based on electrolyte-gated organic thin-film transistor (EGOTFT) has been developed for the detection at the physical limit of C-reactive protein (CRP) in human saliva. CRP is a key relevant biomarker for inflammatory processes and is routinely monitored for many clinical purposes. Herein, an electrolyte-gated thin-film transistor (EGOTFT) has been proposed as a transducer of the biorecognition event taking place at the gate electrode, functionalized with a self-assembled monolayer (SAM) of highly densely packed capturing anti-CRP proteins. Thanks to the SAM, the biosensing platform herein proposed is endowed with ultra-high sensitivity, along with an extremely high selectivity, assessed by measuring the dose curves of CRP interacting with a bovine serum albumin-functionalized gate. Moreover, the biosensing platform is compatible with low-cost fabrication techniques and applicable to the ultra-sensitive detection of a plethora of clinically relevant biomarkers. Therefore, the EGOTFT device herein proposed, being able to operate in physiologically relevant fluids such as saliva, will set the ground to a major revolution in biosensing applications for early clinical detection.
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Citations by publishers
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1 publication, 1.47%
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Frontiers Media S.A.
1 publication, 1.47%
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1 publication, 1.47%
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Scientific Scholar
1 publication, 1.47%
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The Electrochemical Society of Japan
1 publication, 1.47%
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Autonomous Non-profit Organization Editorial Board of the journal Uspekhi Khimii
1 publication, 1.47%
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- We do not take into account publications without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
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Macchia E. et al. Selective single-molecule analytical detection of C-reactive protein in saliva with an organic transistor // Analytical and Bioanalytical Chemistry. 2019. Vol. 411. No. 19. pp. 4899-4908.
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Macchia E., Manoli K., Holzer B., Di Franco C., Picca R. A., Cioffi N., Scamarcio G., Palazzo G., Torsi L. Selective single-molecule analytical detection of C-reactive protein in saliva with an organic transistor // Analytical and Bioanalytical Chemistry. 2019. Vol. 411. No. 19. pp. 4899-4908.
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TY - JOUR
DO - 10.1007/s00216-019-01778-2
UR - https://doi.org/10.1007/s00216-019-01778-2
TI - Selective single-molecule analytical detection of C-reactive protein in saliva with an organic transistor
T2 - Analytical and Bioanalytical Chemistry
AU - Macchia, Eleonora
AU - Manoli, Kyriaki
AU - Holzer, Brigitte
AU - Di Franco, Cinzia
AU - Picca, Rosaria A
AU - Cioffi, Nicola
AU - Scamarcio, Gaetano
AU - Palazzo, Gerardo
AU - Torsi, Luisa
PY - 2019
DA - 2019/03/28 00:00:00
PB - Springer Nature
SP - 4899-4908
IS - 19
VL - 411
PMID - 30923859
SN - 1618-2642
SN - 1618-2650
ER -
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@article{2019_Macchia,
author = {Eleonora Macchia and Kyriaki Manoli and Brigitte Holzer and Cinzia Di Franco and Rosaria A Picca and Nicola Cioffi and Gaetano Scamarcio and Gerardo Palazzo and Luisa Torsi},
title = {Selective single-molecule analytical detection of C-reactive protein in saliva with an organic transistor},
journal = {Analytical and Bioanalytical Chemistry},
year = {2019},
volume = {411},
publisher = {Springer Nature},
month = {mar},
url = {https://doi.org/10.1007/s00216-019-01778-2},
number = {19},
pages = {4899--4908},
doi = {10.1007/s00216-019-01778-2}
}
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Macchia, Eleonora, et al. “Selective single-molecule analytical detection of C-reactive protein in saliva with an organic transistor.” Analytical and Bioanalytical Chemistry, vol. 411, no. 19, Mar. 2019, pp. 4899-4908. https://doi.org/10.1007/s00216-019-01778-2.