том 411 издание 25 страницы 6667-6676

Electrochemical aptasensor for the detection of HER2 in human serum to assist in the diagnosis of early stage breast cancer

Giselda Bezerra 1
Carolina Córdula 1
Danielly Campos 1
Gustavo Nascimento 1
Natália Oliveira 1
Maria Aparecida Seabra 1
Valeria Visani 1
Lucas Sampaio 1
Iasmim Lopes 1
Joana Santos 1
Francisco Xavier 1
Maria Amélia Borba 1
Danyelly Martins 1
José Lima Filho 1
Тип публикацииJournal Article
Дата публикации2019-08-05
scimago Q2
wos Q1
БС1
SJR0.716
CiteScore7.9
Impact factor3.8
ISSN16182642, 16182650
Biochemistry
Analytical Chemistry
Краткое описание
Human epidermal growth factor receptor-2 (HER2) is an important biomarker in the diagnosis and prognosis of breast cancer. This work aimed to develop an aptasensor to detect HER2 in human serum. HER2 aptamer was immobilized by electrostatic adsorption on the surface of a homemade screen-printed electrode modified with poly-l-lysine. Measurements were made by differential pulse voltammetry using methylene blue as a redox indicator. A calibration curve was constructed (R2 = 0.997) using different concentrations of HER2 protein (10–60 ng/mL) in PBS buffer (pH 7.4), with a detection limit of 3.0 ng/mL. The aptasensor showed good reproducibility with relative standard deviations (RSDs) of 3% and remained stable for 3 days with an RSD around 2%. When the tests were performed with serum from a healthy woman, a peak of 6.72 μA was found without the addition of the protein. When we tested the serum of a woman with HER2+ breast cancer, we obtained a signal of 2.65 μA; the same pattern was found when adding to protein in serum control, i.e., the higher the concentration of protein, the lower the signal. The aptasensor was characterized by scanning electron microscopy and isothermal titration calorimetry (ITC), showing excellent interaction between aptamer and target protein. The results revealed a promising and sensitive tool capable of detecting HER2 protein in human serum with albumin depletion, aiding in the molecular diagnosis of breast cancer.
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Bezerra G. et al. Electrochemical aptasensor for the detection of HER2 in human serum to assist in the diagnosis of early stage breast cancer // Analytical and Bioanalytical Chemistry. 2019. Vol. 411. No. 25. pp. 6667-6676.
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Bezerra G., Córdula C., Campos D., Nascimento G., Oliveira N., Seabra M. A., Visani V., Sampaio L., Lopes I., Santos J., Xavier F., Borba M. A., Martins D., Lima Filho J. Electrochemical aptasensor for the detection of HER2 in human serum to assist in the diagnosis of early stage breast cancer // Analytical and Bioanalytical Chemistry. 2019. Vol. 411. No. 25. pp. 6667-6676.
RIS |
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TY - JOUR
DO - 10.1007/s00216-019-02040-5
UR - https://doi.org/10.1007/s00216-019-02040-5
TI - Electrochemical aptasensor for the detection of HER2 in human serum to assist in the diagnosis of early stage breast cancer
T2 - Analytical and Bioanalytical Chemistry
AU - Bezerra, Giselda
AU - Córdula, Carolina
AU - Campos, Danielly
AU - Nascimento, Gustavo
AU - Oliveira, Natália
AU - Seabra, Maria Aparecida
AU - Visani, Valeria
AU - Sampaio, Lucas
AU - Lopes, Iasmim
AU - Santos, Joana
AU - Xavier, Francisco
AU - Borba, Maria Amélia
AU - Martins, Danyelly
AU - Lima Filho, José
PY - 2019
DA - 2019/08/05
PB - Springer Nature
SP - 6667-6676
IS - 25
VL - 411
PMID - 31384983
SN - 1618-2642
SN - 1618-2650
ER -
BibTex |
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BibTex (до 50 авторов) Скопировать
@article{2019_Bezerra,
author = {Giselda Bezerra and Carolina Córdula and Danielly Campos and Gustavo Nascimento and Natália Oliveira and Maria Aparecida Seabra and Valeria Visani and Lucas Sampaio and Iasmim Lopes and Joana Santos and Francisco Xavier and Maria Amélia Borba and Danyelly Martins and José Lima Filho},
title = {Electrochemical aptasensor for the detection of HER2 in human serum to assist in the diagnosis of early stage breast cancer},
journal = {Analytical and Bioanalytical Chemistry},
year = {2019},
volume = {411},
publisher = {Springer Nature},
month = {aug},
url = {https://doi.org/10.1007/s00216-019-02040-5},
number = {25},
pages = {6667--6676},
doi = {10.1007/s00216-019-02040-5}
}
MLA
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Bezerra, Giselda, et al. “Electrochemical aptasensor for the detection of HER2 in human serum to assist in the diagnosis of early stage breast cancer.” Analytical and Bioanalytical Chemistry, vol. 411, no. 25, Aug. 2019, pp. 6667-6676. https://doi.org/10.1007/s00216-019-02040-5.