Analytical and Bioanalytical Chemistry, volume 412, issue 4, pages 811-818

Ultra-low HIV-1 p24 detection limits with a bioelectronic sensor

Eleonora Macchia ^{1, 2}

Lucia Sarcina ¹

Rosaria Anna Picca ¹

Kyriaki Manoli ¹

Cinzia Di Franco ¹

Gaetano Scamarcio ^{3, 4}

Luisa Torsi ^{1, 2, 5}

Hide authors affiliations Show authors affiliations: 5 affiliations

Dipartimento Di Chimica, Università Degli Studi Di Bari Aldo Moro, Bari, Italy |

The Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland |

Dipartimento Interateneo di Fisica “M. Merlin”, Università degli Studi di Bari Aldo Moro, Bari, Italy |

⁴

Unità di Bari, CNR - Istituto di Fotonica e Nanotecnologie, Bari, Italy |

⁵

CSGI (Centre for Colloid and Surface Science), Bari, Italy |

Publication type: Journal Article

Publication date: 2019-12-21

Springer Nature

Journal: Analytical and Bioanalytical Chemistry

Quartile SCImago

Quartile WOS

Impact factor: 4.3

ISSN: 16182642, 16182650

DOI: 10.1007/s00216-019-02319-7

Copy DOI

Biochemistry

Analytical Chemistry

Abstract

Early diagnosis of the infection caused by human immunodeficiency virus type-1 (HIV-1) is vital to achieve efficient therapeutic treatment and limit the disease spreading when the viremia is at its highest level. To this end, a point-of-care HIV-1 detection carried out with label-free, low-cost, and ultra-sensitive screening technologies would be of great relevance. Herein, a label-free single molecule detection of HIV-1 p24 capsid protein with a large (wide-field) single-molecule transistor (SiMoT) sensor is proposed. The system is based on an electrolyte-gated field-effect transistor whose gate is bio-functionalized with the antibody against the HIV-1 p24 capsid protein. The device exhibits a limit of detection of a single protein and a limit of quantification in the 10 molecule range. This study paves the way for a low-cost technology that can quantify, with single-molecule precision, the transition of a biological organism from being “healthy” to being “diseased” by tracking a target biomarker. This can open to the possibility of performing the earliest possible diagnosis.

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Macchia E. et al. Ultra-low HIV-1 p24 detection limits with a bioelectronic sensor // Analytical and Bioanalytical Chemistry. 2019. Vol. 412. No. 4. pp. 811-818.

GOST all authors (up to 50) Copy

Macchia E., Sarcina L., Picca R. A., Manoli K., Di Franco C., Scamarcio G., Torsi L. Ultra-low HIV-1 p24 detection limits with a bioelectronic sensor // Analytical and Bioanalytical Chemistry. 2019. Vol. 412. No. 4. pp. 811-818.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1007/s00216-019-02319-7

UR - https://doi.org/10.1007/s00216-019-02319-7

TI - Ultra-low HIV-1 p24 detection limits with a bioelectronic sensor

T2 - Analytical and Bioanalytical Chemistry

AU - Macchia, Eleonora

AU - Sarcina, Lucia

AU - Picca, Rosaria Anna

AU - Manoli, Kyriaki

AU - Di Franco, Cinzia

AU - Scamarcio, Gaetano

AU - Torsi, Luisa

PY - 2019

DA - 2019/12/21 00:00:00

PB - Springer Nature

SP - 811-818

IS - 4

VL - 412

SN - 1618-2642

SN - 1618-2650

ER -

BibTex |

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BibTex Copy

@article{2019_Macchia,

author = {Eleonora Macchia and Lucia Sarcina and Rosaria Anna Picca and Kyriaki Manoli and Cinzia Di Franco and Gaetano Scamarcio and Luisa Torsi},

title = {Ultra-low HIV-1 p24 detection limits with a bioelectronic sensor},

journal = {Analytical and Bioanalytical Chemistry},

year = {2019},

volume = {412},

publisher = {Springer Nature},

month = {dec},

url = {https://doi.org/10.1007/s00216-019-02319-7},

number = {4},

pages = {811--818},

doi = {10.1007/s00216-019-02319-7}

}

MLA

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Macchia, Eleonora, et al. “Ultra-low HIV-1 p24 detection limits with a bioelectronic sensor.” Analytical and Bioanalytical Chemistry, vol. 412, no. 4, Dec. 2019, pp. 811-818. https://doi.org/10.1007/s00216-019-02319-7.

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Publisher

Springer Nature

Journal

Analytical and Bioanalytical Chemistry

Quartile SCImago

Quartile WOS

Impact factor

4.3

ISSN

16182642 (Print)
16182650 (Electronic)