Analytical and Bioanalytical Chemistry, volume 413, issue 21, pages 5333-5347

Differentiation of Central Slovenian and Moscow populations of Rana temporaria frogs using peptide biomarkers of temporins family

Samgina T Yu ¹

Vasileva I D ¹

Kovalev S V ¹

Trebše P. ²

Torkar G ³

Surin A K ⁴

Zubarev R A ^{5, 6}

Lebedev A. T. ¹

Hide authors affiliations Show authors affiliations: 6 affiliations

Department of Organic Chemistry, Lomonosov Moscow State University, Moscow, Russia |

University of Ljubljana Faculty of Health Sciences, Ljubljana, Slovenia |

Department for Biology, Chemistry and Home Economics, University of Ljubljana Faculty of Education, Ljubljana, Slovenia |

⁴

Pushchino Branch, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia |

⁵

Department of Medicinal Biochemistry and Biophysics, Division of Molecular Biometry, Karolinska Institutet, Stockholm, Sweden |

⁶

Department of Pharmacological & Technological Chemistry, I.M. Sechenov First Moscow State Medical University, Moscow, Russia |

Publication type: Journal Article

Publication date: 2021-07-07

Springer Nature

Journal: Analytical and Bioanalytical Chemistry

Quartile SCImago

Quartile WOS

Impact factor: 4.3

ISSN: 16182642, 16182650

DOI: 10.1007/s00216-021-03506-1

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Biochemistry

Analytical Chemistry

Abstract

Skin secretion represents the only means of defense for the majority of frog species. That phenomenon is based on the fact that the main components of the secretion are peptides demonstrating greatly varying types of bioactivity. They fulfill regulatory functions, fight microorganisms and may be even helpful against predators. These peptides are considered to be rather promising pharmaceuticals of future generation as according to the present knowledge microorganisms are unlikely to develop resistance to them. Mass spectrometry sequencing of these peptides is the most efficient first step of their study providing reliably their primary structures, i.e., amino acids sequence and S-S bond motif. Besides discovering new bioactive peptides, mass spectrometry appears to be an efficient tool of taxonomy studies, allowing for distinguishing not only between closely related species, but also between populations of the same species. Application of several tandem mass spectrometry tools (CID, HCD, ETD, EThcD) available with Orbitrap mass analyzer allowed us to obtain full sequence of about 60 peptides in the secretion of Slovenian population of brown ranid frog Rana temporaria. The problem of sequence inside C-terminal cycle formed by two Cys and differentiation of isomeric Leu and Ile residues was done in top-down mode without any derivatization steps. Besides general biomarkers of Rana temporaria species, Central Slovenian population of Rana temporaria demonstrates six novel temporins and one brevinin 1, which may be treated as biomarkers of that population.

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Citations by journals

	1 2
Journal of the American Society for Mass Spectrometry	Journal of the American Society for Mass Spectrometry, 2, 40% Journal of the American Society for Mass Spectrometry 2 publications, 40%
International Journal of Mass Spectrometry	International Journal of Mass Spectrometry, 1, 20% International Journal of Mass Spectrometry 1 publication, 20%
International Journal of Molecular Sciences	International Journal of Molecular Sciences, 1, 20% International Journal of Molecular Sciences 1 publication, 20%
Journal of Analytical Chemistry	Journal of Analytical Chemistry, 1, 20% Journal of Analytical Chemistry 1 publication, 20%
	1 2

Citations by publishers

	1 2
American Chemical Society (ACS)	American Chemical Society (ACS), 2, 40% American Chemical Society (ACS) 2 publications, 40%
Elsevier	Elsevier, 1, 20% Elsevier 1 publication, 20%
Multidisciplinary Digital Publishing Institute (MDPI)	Multidisciplinary Digital Publishing Institute (MDPI), 1, 20% Multidisciplinary Digital Publishing Institute (MDPI) 1 publication, 20%
Pleiades Publishing	Pleiades Publishing, 1, 20% Pleiades Publishing 1 publication, 20%
	1 2

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Samgina T. Yu. et al. Differentiation of Central Slovenian and Moscow populations of Rana temporaria frogs using peptide biomarkers of temporins family // Analytical and Bioanalytical Chemistry. 2021. Vol. 413. No. 21. pp. 5333-5347.

GOST all authors (up to 50) Copy

Samgina T. Yu., Vasileva I. D., Kovalev S. V., Trebše P., Torkar G., Surin A. K., Zubarev R. A., Lebedev A. T. Differentiation of Central Slovenian and Moscow populations of Rana temporaria frogs using peptide biomarkers of temporins family // Analytical and Bioanalytical Chemistry. 2021. Vol. 413. No. 21. pp. 5333-5347.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1007/s00216-021-03506-1

UR - https://doi.org/10.1007%2Fs00216-021-03506-1

TI - Differentiation of Central Slovenian and Moscow populations of Rana temporaria frogs using peptide biomarkers of temporins family

T2 - Analytical and Bioanalytical Chemistry

AU - Samgina, T Yu

AU - Vasileva, I D

AU - Kovalev, S V

AU - Trebše, P.

AU - Torkar, G

AU - Surin, A K

AU - Zubarev, R A

AU - Lebedev, A. T.

PY - 2021

DA - 2021/07/07 00:00:00

PB - Springer Nature

SP - 5333-5347

IS - 21

VL - 413

SN - 1618-2642

SN - 1618-2650

ER -

BibTex |

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BibTex Copy

@article{2021_Samgina,

author = {T Yu Samgina and I D Vasileva and S V Kovalev and P. Trebše and G Torkar and A K Surin and R A Zubarev and A. T. Lebedev},

title = {Differentiation of Central Slovenian and Moscow populations of Rana temporaria frogs using peptide biomarkers of temporins family},

journal = {Analytical and Bioanalytical Chemistry},

year = {2021},

volume = {413},

publisher = {Springer Nature},

month = {jul},

url = {https://doi.org/10.1007%2Fs00216-021-03506-1},

number = {21},

pages = {5333--5347},

doi = {10.1007/s00216-021-03506-1}

}

MLA

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MLA Copy

Samgina, T. Yu., et al. “Differentiation of Central Slovenian and Moscow populations of Rana temporaria frogs using peptide biomarkers of temporins family.” Analytical and Bioanalytical Chemistry, vol. 413, no. 21, Jul. 2021, pp. 5333-5347. https://doi.org/10.1007%2Fs00216-021-03506-1.

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Publisher

Springer Nature

Journal

Analytical and Bioanalytical Chemistry

Quartile SCImago

Quartile WOS

Impact factor

4.3

ISSN

16182642 (Print)
16182650 (Electronic)

Labs

Laboratory of physico-chemical methods for the analysis of the structure of matter

Profiles

Lebedev, Albert T