Fluorometric determination and intracellular imaging of cysteine by using glutathione capped gold nanoclusters and cerium(III) induced aggregation
Publication type: Journal Article
Publication date: 2019-05-03
scimago Q1
wos Q1
SJR: 0.833
CiteScore: 7.8
Impact factor: 5.3
ISSN: 00263672, 14365073
PubMed ID:
31053973
Analytical Chemistry
Abstract
A turn-on fluorometric method is described for selective and sensitive detection of cysteine (Cys). Gold nanoclusters (Au NCs) capped with glutathione (GSH) are used as a fluorescent probe. If Ce3+ ion are present, they will bind to the carboxy groups of the GSH-capped Au NC. This results in aggregation-induced emission enhancement (AIEE), best measured at excitation/emission wavelengths of 360/575 nm. On addition of Cys, which has less steric hindrance compared with GSH and higher affinity for Ce3+, it will bind to Ce3+ through the carboxyl group and link with Au NCs via Au-S bond. Hence, the AIEE is increased and Cys can be quantified via this effect with a linear response in the 0.4–120 μmol L−1 Cys concentration range and a detection limit of 0.15 μmol L−1.
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Total citations:
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Citations from 2025:
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(9.09%)
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GOST
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Lai Q. et al. Fluorometric determination and intracellular imaging of cysteine by using glutathione capped gold nanoclusters and cerium(III) induced aggregation // Microchimica Acta. 2019. Vol. 186. No. 6. 327
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Lai Q., Liu Q., Zhao K., Duan X., Wang G., Su X. Fluorometric determination and intracellular imaging of cysteine by using glutathione capped gold nanoclusters and cerium(III) induced aggregation // Microchimica Acta. 2019. Vol. 186. No. 6. 327
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TY - JOUR
DO - 10.1007/s00604-019-3438-1
UR - https://doi.org/10.1007/s00604-019-3438-1
TI - Fluorometric determination and intracellular imaging of cysteine by using glutathione capped gold nanoclusters and cerium(III) induced aggregation
T2 - Microchimica Acta
AU - Lai, Qi
AU - Liu, Qing
AU - Zhao, Kai
AU - Duan, Xinhe
AU - Wang, Guannan
AU - Su, Xingguang
PY - 2019
DA - 2019/05/03
PB - Springer Nature
IS - 6
VL - 186
PMID - 31053973
SN - 0026-3672
SN - 1436-5073
ER -
Cite this
BibTex (up to 50 authors)
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@article{2019_Lai,
author = {Qi Lai and Qing Liu and Kai Zhao and Xinhe Duan and Guannan Wang and Xingguang Su},
title = {Fluorometric determination and intracellular imaging of cysteine by using glutathione capped gold nanoclusters and cerium(III) induced aggregation},
journal = {Microchimica Acta},
year = {2019},
volume = {186},
publisher = {Springer Nature},
month = {may},
url = {https://doi.org/10.1007/s00604-019-3438-1},
number = {6},
pages = {327},
doi = {10.1007/s00604-019-3438-1}
}