Springer Nature
European Journal of Clinical Microbiology and Infectious Diseases
,  volume 43 ,  issue 7 ,  pages 1427-1436

Performance of the Roche Elecsys® IGRA SARS-CoV-2 test for the detection and quantification of virus-reactive T cells in COVID-19-vaccinated immunosuppressed patients and healthy subjects

Diego Carretero 1
, 
Estela Giménez 1
, 
Eliseo Albert 1
, 
Ester Colomer 1
, 
Marco Montomoli 2
, 
Rafael Hernani 3
, 
José Luis Piñana 3
, 
José Luis Górriz 2, 4
, 
Carlos Solano 3, 4
, 
David Navarro 1, 5, 6, 7
1
 
2
 
3
 
4
 
5
 
6
 
7
 
Publication type: Journal Article
Publication date: 2024-05-23
Springer Nature
scimago Q1
wos Q2
SJR: 0.986
CiteScore: 7.3
Impact factor: 3.0
ISSN: 09349723, 14354373
Abstract
Purpose

Comparing the performance of commercially available SARS-CoV-2 T-cell immunoassay responses may provide useful information for future observational or intervention studies as well as to their potential customers.

Method

Whole blood was collected from a total of 183 subjects fully vaccinated against COVID-19: 55 healthy controls (Group 1), 50 hematological patients (Group 2), 50 chronic kidney disease patients (Group 3), and 28 elderly nursing home residents (Group 4). Samples were tested with the Roche Elecsys® IGRA (Interferon-gamma release assay) SARS-CoV-2 test (Roche Diagnostics, Rotkreuz, Switzerland), the Euroimmun SARS-CoV-2 test (Euroimmun, Lubeck, Germany), the SARS-CoV-2 T Cell Analysis Kit (Miltenyi Biotec, Bergisch Gladbach, Germany), and a flow-cytometry for intracellular cytokine (IFN-γ) staining-based immunoassay (FC-ICS).

Results

Overall, the Roche Elecsys® assay returned the highest number of positive results (151/179; 84.3%), followed by the Euroimmun test (127/183; 69%), and the FC-ICS (135/179; 75%). The Kappa coefficient of agreement was best between IGRAs (0.64). Most discordant results across assays involved patients from Group 2. Overall, IFN-γ concentrations measured by both IGRAs correlated strongly (rho = 0.78; 95% CI 0.71–0.84; P < 0.001) irrespective of the study group. The frequencies of SARS-CoV-2-reactive IFN-γ T cells and IFN-γ concentrations measured by the IGRAs correlated moderately for CD4+ T cells, however, weakly for CD8+ T cells. SARS-CoV-2-experienced participants displayed stronger responses than SARS-CoV-2-naïve when IGRAs, rather than FC-ICS, were used.

Conclusion

The SARS-CoV-2 immunoassays evaluated in the present study did not return interchangeable qualitative or quantitative results either in seemingly healthy individuals or in immunosuppressed patients.

Found 
Found 

Top-30

Publishers

1
Cold Spring Harbor Laboratory
Cold Spring Harbor Laboratory, 1, 100%
Cold Spring Harbor Laboratory
1 publication, 100%
1
  • We do not take into account publications without a DOI.
  • Statistics recalculated weekly.

Are you a researcher?

Create a profile to get free access to personal recommendations for colleagues and new articles.
Metrics
1
Share
Cite this
GOST |
Cite this
GOST Copy
Carretero D. et al. Performance of the Roche Elecsys® IGRA SARS-CoV-2 test for the detection and quantification of virus-reactive T cells in COVID-19-vaccinated immunosuppressed patients and healthy subjects // European Journal of Clinical Microbiology and Infectious Diseases. 2024. Vol. 43. No. 7. pp. 1427-1436.
GOST all authors (up to 50) Copy
Carretero D., Giménez E., Albert E., Colomer E., Montomoli M., Hernani R., Piñana J. L., Górriz J. L., Solano C., Navarro D. Performance of the Roche Elecsys® IGRA SARS-CoV-2 test for the detection and quantification of virus-reactive T cells in COVID-19-vaccinated immunosuppressed patients and healthy subjects // European Journal of Clinical Microbiology and Infectious Diseases. 2024. Vol. 43. No. 7. pp. 1427-1436.
RIS |
Cite this
RIS Copy
TY - JOUR
DO - 10.1007/s10096-024-04852-5
UR - https://link.springer.com/10.1007/s10096-024-04852-5
TI - Performance of the Roche Elecsys® IGRA SARS-CoV-2 test for the detection and quantification of virus-reactive T cells in COVID-19-vaccinated immunosuppressed patients and healthy subjects
T2 - European Journal of Clinical Microbiology and Infectious Diseases
AU - Carretero, Diego
AU - Giménez, Estela
AU - Albert, Eliseo
AU - Colomer, Ester
AU - Montomoli, Marco
AU - Hernani, Rafael
AU - Piñana, José Luis
AU - Górriz, José Luis
AU - Solano, Carlos
AU - Navarro, David
PY - 2024
DA - 2024/05/23
PB - Springer Nature
SP - 1427-1436
IS - 7
VL - 43
PMID - 38777919
SN - 0934-9723
SN - 1435-4373
ER -
BibTex |
Cite this
BibTex (up to 50 authors) Copy
@article{2024_Carretero,
author = {Diego Carretero and Estela Giménez and Eliseo Albert and Ester Colomer and Marco Montomoli and Rafael Hernani and José Luis Piñana and José Luis Górriz and Carlos Solano and David Navarro},
title = {Performance of the Roche Elecsys® IGRA SARS-CoV-2 test for the detection and quantification of virus-reactive T cells in COVID-19-vaccinated immunosuppressed patients and healthy subjects},
journal = {European Journal of Clinical Microbiology and Infectious Diseases},
year = {2024},
volume = {43},
publisher = {Springer Nature},
month = {may},
url = {https://link.springer.com/10.1007/s10096-024-04852-5},
number = {7},
pages = {1427--1436},
doi = {10.1007/s10096-024-04852-5}
}
MLA
Cite this
MLA Copy
Carretero, Diego, et al. “Performance of the Roche Elecsys® IGRA SARS-CoV-2 test for the detection and quantification of virus-reactive T cells in COVID-19-vaccinated immunosuppressed patients and healthy subjects.” European Journal of Clinical Microbiology and Infectious Diseases, vol. 43, no. 7, May. 2024, pp. 1427-1436. https://link.springer.com/10.1007/s10096-024-04852-5.