Bulletin of Experimental Biology and Medicine, volume 167, issue 1, pages 123-130
Preparation and Testing of Cells Expressing Fluorescent Proteins for Intravital Imaging of Tumor Microenvironment
Vodop’yanov S. S.
1
,
Kunin M A
2
,
Garanina A S
1
,
Grinenko N F
3
,
Vlasova K Yu
2
,
Melnikov P A
3
,
Chekhonin V. P.
3, 4
,
Sukhinich K K
5
,
Makarov A. V.
3
,
Naumenko V A
1
,
Abakumov M A
1, 4
,
Majouga A G
1, 2, 6
5
Publication type: Journal Article
Publication date: 2019-05-01
Quartile SCImago
Q3
Quartile WOS
Q4
Impact factor: 0.7
ISSN: 00074888, 15738221, 03659615, 24131008
General Biochemistry, Genetics and Molecular Biology
General Medicine
Abstract
Intravital microscopy is widely used for in vivo studies of the mechanisms of carcinogenesis and response to antitumor therapy. For visualization of tumor cells in vivo, cell lines expressing fluorescent proteins are needed. Expression of exogenous proteins can affect cell growth rate and their tumorigenic potential. Therefore, comprehensive analysis of the morphofunctional properties of transduced cells is required for creating appropriate models of tumor microenvironment. In the present study, six lines of mouse tumor cells expressing green and red fluorescent proteins were derived. Analysis of cells morphology, growth kinetics, and response to chemotherapy in vitro revealed no significant differences between wild-type and transduced cell lines. Introduction of fluorescent proteins into the genome of 4T1 (murine breast cancer) and B16-F10 (murine melanoma) cells did not affect tumor growth rate after subcutaneous implantation to mice, while both CT26-GFP and CT26-RFP cells (murine colon cancer) were rejected starting from day 8 after implantation. Elucidation of the mechanisms underlying CT26-GFP/RFP rejection is required to modify transduction technique for creating the models of tumor microenvironment accessible for in vivo visualization. Transduced 4T1 and B16-F10 cell lines can be used for intravital microscopic imaging of tumor cells, neoplastic vasculature, and leukocyte subpopulations.
Citations by journals
|
1
|
|
|
Acta Biomaterialia
|
Acta Biomaterialia
1 publication, 16.67%
|
|
Journal of Controlled Release
|
Journal of Controlled Release
1 publication, 16.67%
|
|
Nanomaterials
|
Nanomaterials
1 publication, 16.67%
|
|
Pharmaceuticals
|
Pharmaceuticals
1 publication, 16.67%
|
|
Journal of Fluorescence
|
Journal of Fluorescence
1 publication, 16.67%
|
|
1
|
Citations by publishers
|
1
2
|
|
|
Elsevier
|
Elsevier
2 publications, 33.33%
|
|
Multidisciplinary Digital Publishing Institute (MDPI)
|
Multidisciplinary Digital Publishing Institute (MDPI)
2 publications, 33.33%
|
|
Springer Nature
|
Springer Nature
1 publication, 16.67%
|
|
1
2
|
- We do not take into account publications that without a DOI.
- Statistics recalculated only for publications connected to researchers, organizations and labs registered on the platform.
- Statistics recalculated weekly.
{"yearsCitations":{"type":"bar","data":{"show":true,"labels":[2020,2021,2022,2023,2024],"ids":[0,0,0,0,0],"codes":[0,0,0,0,0],"imageUrls":["","","","",""],"datasets":[{"label":"Citations number","data":[1,1,1,2,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":["16.67","16.67","16.67","33.33","16.67"],"barThickness":null}]},"options":{"indexAxis":"x","maintainAspectRatio":true,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":1,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Citations per year","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"journals":{"type":"bar","data":{"show":true,"labels":["Acta Biomaterialia","Journal of Controlled Release","Nanomaterials","Pharmaceuticals","Journal of Fluorescence"],"ids":[9874,14351,10051,1203,21006],"codes":[0,0,0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp"],"datasets":[{"label":"","data":[1,1,1,1,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6","#3B82F6","#3B82F6"],"percentage":[16.67,16.67,16.67,16.67,16.67],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Journals","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}},"publishers":{"type":"bar","data":{"show":true,"labels":["Elsevier","Multidisciplinary Digital Publishing Institute (MDPI)","Springer Nature"],"ids":[17,202,8],"codes":[0,0,0],"imageUrls":["\/storage\/images\/resized\/GDnYOu1UpMMfMMRV6Aqle4H0YLLsraeD9IP9qScG_medium.webp","\/storage\/images\/resized\/MjH1ITP7lMYGxeqUZfkt2BnVLgjkk413jwBV97XX_medium.webp","\/storage\/images\/resized\/voXLqlsvTwv5p3iMQ8Dhs95nqB4AXOG7Taj7G4ra_medium.webp"],"datasets":[{"label":"","data":[2,2,1],"backgroundColor":["#3B82F6","#3B82F6","#3B82F6"],"percentage":[33.33,33.33,16.67],"barThickness":13}]},"options":{"indexAxis":"y","maintainAspectRatio":false,"scales":{"y":{"ticks":{"precision":0,"autoSkip":false,"font":{"family":"Montserrat"},"color":"#000000"}},"x":{"ticks":{"stepSize":null,"precision":0,"font":{"family":"Montserrat"},"color":"#000000"}}},"plugins":{"legend":{"position":"top","labels":{"font":{"family":"Montserrat"},"color":"#000000"}},"title":{"display":true,"text":"Publishers","font":{"size":24,"family":"Montserrat","weight":600},"color":"#000000"}}}}}
Metrics
Cite this
GOST |
RIS |
BibTex |
MLA
Cite this
GOST
Copy
Vodop’yanov S. S. et al. Preparation and Testing of Cells Expressing Fluorescent Proteins for Intravital Imaging of Tumor Microenvironment // Bulletin of Experimental Biology and Medicine. 2019. Vol. 167. No. 1. pp. 123-130.
GOST all authors (up to 50)
Copy
Vodop’yanov S. S., Kunin M. A., Garanina A. S., Grinenko N. F., Vlasova K. Yu., Melnikov P. A., Chekhonin V. P., Sukhinich K. K., Makarov A. V., Naumenko V. A., Abakumov M. A., Majouga A. G. Preparation and Testing of Cells Expressing Fluorescent Proteins for Intravital Imaging of Tumor Microenvironment // Bulletin of Experimental Biology and Medicine. 2019. Vol. 167. No. 1. pp. 123-130.
Cite this
RIS
Copy
TY - JOUR
DO - 10.1007/s10517-019-04475-3
UR - https://doi.org/10.1007%2Fs10517-019-04475-3
TI - Preparation and Testing of Cells Expressing Fluorescent Proteins for Intravital Imaging of Tumor Microenvironment
T2 - Bulletin of Experimental Biology and Medicine
AU - Vodop’yanov, S. S.
AU - Kunin, M A
AU - Garanina, A S
AU - Grinenko, N F
AU - Vlasova, K Yu
AU - Melnikov, P A
AU - Chekhonin, V. P.
AU - Sukhinich, K K
AU - Makarov, A. V.
AU - Naumenko, V A
AU - Majouga, A G
AU - Abakumov, M A
PY - 2019
DA - 2019/05/01 00:00:00
PB - Springer Nature
SP - 123-130
IS - 1
VL - 167
SN - 0007-4888
SN - 1573-8221
SN - 0365-9615
SN - 2413-1008
ER -
Cite this
BibTex
Copy
@article{2019_Vodop’yanov,
author = {S. S. Vodop’yanov and M A Kunin and A S Garanina and N F Grinenko and K Yu Vlasova and P A Melnikov and V. P. Chekhonin and K K Sukhinich and A. V. Makarov and V A Naumenko and A G Majouga and M A Abakumov},
title = {Preparation and Testing of Cells Expressing Fluorescent Proteins for Intravital Imaging of Tumor Microenvironment},
journal = {Bulletin of Experimental Biology and Medicine},
year = {2019},
volume = {167},
publisher = {Springer Nature},
month = {may},
url = {https://doi.org/10.1007%2Fs10517-019-04475-3},
number = {1},
pages = {123--130},
doi = {10.1007/s10517-019-04475-3}
}
Cite this
MLA
Copy
Vodop’yanov, S. S., et al. “Preparation and Testing of Cells Expressing Fluorescent Proteins for Intravital Imaging of Tumor Microenvironment.” Bulletin of Experimental Biology and Medicine, vol. 167, no. 1, May. 2019, pp. 123-130. https://doi.org/10.1007%2Fs10517-019-04475-3.