BioMetals, volume 32, issue 2, pages 265-272
An engineered GFP fluorescent bacterial biosensor for detecting and quantifying silver and copper ions
3
Metagenom Bio Inc, Toronto, Canada
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Publication type: Journal Article
Publication date: 2019-02-13
Metals and Alloys
General Biochemistry, Genetics and Molecular Biology
Biomaterials
General Agricultural and Biological Sciences
Abstract
Presented here are two engineered bacterial biosensors for detecting and quantifying silver and copper ions. The biosensors contain a silver/copper resistance operon and a Green Fluorescent Protein gene that is strictly regulated through silver activated promoter regions normally found on a silver resistance gene (sil operon). The two biosensors efficiently detected silver and copper concentrations of 40 µM–300 µM and 20 µM–600 µM respectively. A strong correlation (R2 = 0.90 or above) between silver/copper and GFP signal makes it possible to quantify the ions using a linear regression. At room temperature incubation, the GFP signal of the biosensors in Ag+ saturated after 13 h. However, a detectable GFP signal was seen in 4 h.
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Martinez A. R. et al. An engineered GFP fluorescent bacterial biosensor for detecting and quantifying silver and copper ions // BioMetals. 2019. Vol. 32. No. 2. pp. 265-272.
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Martinez A. R., Heil J. R., Charles T. C. An engineered GFP fluorescent bacterial biosensor for detecting and quantifying silver and copper ions // BioMetals. 2019. Vol. 32. No. 2. pp. 265-272.
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TY - JOUR
DO - 10.1007/s10534-019-00179-3
UR - https://doi.org/10.1007/s10534-019-00179-3
TI - An engineered GFP fluorescent bacterial biosensor for detecting and quantifying silver and copper ions
T2 - BioMetals
AU - Martinez, Adam Radek
AU - Heil, John R.
AU - Charles, T. C.
PY - 2019
DA - 2019/02/13 00:00:00
PB - Springer Nature
SP - 265-272
IS - 2
VL - 32
SN - 0966-0844
SN - 1572-8773
ER -
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@article{2019_Martinez,
author = {Adam Radek Martinez and John R. Heil and T. C. Charles},
title = {An engineered GFP fluorescent bacterial biosensor for detecting and quantifying silver and copper ions},
journal = {BioMetals},
year = {2019},
volume = {32},
publisher = {Springer Nature},
month = {feb},
url = {https://doi.org/10.1007/s10534-019-00179-3},
number = {2},
pages = {265--272},
doi = {10.1007/s10534-019-00179-3}
}
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Martinez, Adam Radek, et al. “An engineered GFP fluorescent bacterial biosensor for detecting and quantifying silver and copper ions.” BioMetals, vol. 32, no. 2, Feb. 2019, pp. 265-272. https://doi.org/10.1007/s10534-019-00179-3.