BioMetals

, volume 32 , issue 2 , pages 265-272

An engineered GFP fluorescent bacterial biosensor for detecting and quantifying silver and copper ions

Adam Radek Martinez ¹

John R. Heil ^{1, 2, 3}

T. C. Charles ^{1, 2, 3, 4}

Hide authors affiliations Show authors affiliations: 4 affiliations

Waterloo Centre for Microbial Research, University of Waterloo, Waterloo, Canada |

Department Of Biology, University Of Waterloo, Waterloo, Canada |

Metagenom Bio Inc, Toronto, Canada |

⁴

University Of Waterloo, Waterloo, Canada |

Publication type: Journal Article

Publication date: 2019-02-13

Springer Nature

BioMetals

scimago Q1

wos Q2

SJR: 0.785

CiteScore: 7.7

Impact factor: 3.6

ISSN: 09660844, 15728773

DOI: 10.1007/s10534-019-00179-3

Copy DOI

PubMed ID: 30756218

Metals and Alloys

General Biochemistry, Genetics and Molecular Biology

Biomaterials

General Agricultural and Biological Sciences

Abstract

Presented here are two engineered bacterial biosensors for detecting and quantifying silver and copper ions. The biosensors contain a silver/copper resistance operon and a Green Fluorescent Protein gene that is strictly regulated through silver activated promoter regions normally found on a silver resistance gene (sil operon). The two biosensors efficiently detected silver and copper concentrations of 40 µM–300 µM and 20 µM–600 µM respectively. A strong correlation (R2 = 0.90 or above) between silver/copper and GFP signal makes it possible to quantify the ions using a linear regression. At room temperature incubation, the GFP signal of the biosensors in Ag+ saturated after 13 h. However, a detectable GFP signal was seen in 4 h.

Found

1 citation

Ponomarenko Sergey

🥼 🤝

DSc in Chemistry, associate member of the Russian Academy of Sciences

261 publications, 6 129 citations, 64 reviews

h-index: 41

Lomonosov Moscow State University

Enikolopov Institute of Synthetic Polymeric Materials of the Russian Academy of Sciences

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Martinez A. R. et al. An engineered GFP fluorescent bacterial biosensor for detecting and quantifying silver and copper ions // BioMetals. 2019. Vol. 32. No. 2. pp. 265-272.

GOST all authors (up to 50) Copy

Martinez A. R., Heil J. R., Charles T. C. An engineered GFP fluorescent bacterial biosensor for detecting and quantifying silver and copper ions // BioMetals. 2019. Vol. 32. No. 2. pp. 265-272.

RIS |

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RIS Copy

TY - JOUR

DO - 10.1007/s10534-019-00179-3

UR - https://doi.org/10.1007/s10534-019-00179-3

TI - An engineered GFP fluorescent bacterial biosensor for detecting and quantifying silver and copper ions

T2 - BioMetals

AU - Martinez, Adam Radek

AU - Heil, John R.

AU - Charles, T. C.

PY - 2019

DA - 2019/02/13

PB - Springer Nature

SP - 265-272

IS - 2

VL - 32

PMID - 30756218

SN - 0966-0844

SN - 1572-8773

ER -

BibTex |

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BibTex (up to 50 authors) Copy

@article{2019_Martinez,

author = {Adam Radek Martinez and John R. Heil and T. C. Charles},

title = {An engineered GFP fluorescent bacterial biosensor for detecting and quantifying silver and copper ions},

journal = {BioMetals},

year = {2019},

volume = {32},

publisher = {Springer Nature},

month = {feb},

url = {https://doi.org/10.1007/s10534-019-00179-3},

number = {2},

pages = {265--272},

doi = {10.1007/s10534-019-00179-3}

}

MLA

Cite this

MLA Copy

Martinez, Adam Radek, et al. “An engineered GFP fluorescent bacterial biosensor for detecting and quantifying silver and copper ions.” BioMetals, vol. 32, no. 2, Feb. 2019, pp. 265-272. https://doi.org/10.1007/s10534-019-00179-3.

Publisher

Springer Nature

Journal

BioMetals

scimago Q1

wos Q2

SJR

0.785

CiteScore

7.7

Impact factor

3.6

ISSN

09660844 (Print)

15728773 (Electronic)