BioNanoScience, volume 8, issue 2, pages 701-706
Development of a Sensitive Multiplexed Open Circuit Potential System for the Detection of Prostate Cancer Biomarkers
1
Department of Electronic & Electrical Engineering, University of Bath, Bath, UK
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Publication type: Journal Article
Publication date: 2017-04-01
Journal:
BioNanoScience
Quartile SCImago
Q3
Quartile WOS
—
Impact factor: 2.9
ISSN: 21911630, 21911649
Bioengineering
Biomedical Engineering
Abstract
We report the development of a sensitive label-free, cost-effective detection system with simultaneous multi-channel measurement of open circuit potential (OCP) variations for the detection of prostate specific antigen (PSA). We demonstrate a significant increase of 600 times in the sensitivity as compared to the reported literature. To accurately measure OCP variations, a complete monolithic field-effect transistor (FET)-input ultra-low input bias current instrumentation amplifier is used to form the electronic circuit to measure the variation between a working electrode and a reference electrode. This amplifier electronic system setup provides a differential voltage measurement with high input impedance and low input bias current. Since no current is applied to the electrochemical system, a true and accurate measurement of the variation can be performed. This is the first report on the use of DNA aptamers with an OCP system where we employed a DNA aptamer against PSA. An optimised ratio of anti-PSA DNA aptamer with 6-mercapto-1-hexanol (MCH) was used to fabricate the aptasensor using gold electrodes. The electrodes are hosted in a cell with an automated flow system. A wide range of concentrations of PSA (0.1 to 100 ng/mL) were injected through the system. The sensor could potentially differentiate 0.1 ng/mL PSA from blank measurement, which is well below the required clinical range (>1 ng/mL). The sensor was also challenged with 4% human serum albumin and human kallikrein2 as control proteins where the sensor demonstrated excellent selectivity. The developed system can be further generalised to various other targets using specific probes.
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Citations by publishers
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- We do not take into account publications without a DOI.
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Wong L. C. C., Jolly P., Estrela P. Development of a Sensitive Multiplexed Open Circuit Potential System for the Detection of Prostate Cancer Biomarkers // BioNanoScience. 2017. Vol. 8. No. 2. pp. 701-706.
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Wong L. C. C., Jolly P., Estrela P. Development of a Sensitive Multiplexed Open Circuit Potential System for the Detection of Prostate Cancer Biomarkers // BioNanoScience. 2017. Vol. 8. No. 2. pp. 701-706.
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TY - JOUR
DO - 10.1007/s12668-017-0408-0
UR - https://doi.org/10.1007/s12668-017-0408-0
TI - Development of a Sensitive Multiplexed Open Circuit Potential System for the Detection of Prostate Cancer Biomarkers
T2 - BioNanoScience
AU - Wong, Lai Chun Caleb
AU - Jolly, Pawan
AU - Estrela, Pedro
PY - 2017
DA - 2017/04/01 00:00:00
PB - Springer Nature
SP - 701-706
IS - 2
VL - 8
SN - 2191-1630
SN - 2191-1649
ER -
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@article{2017_Wong,
author = {Lai Chun Caleb Wong and Pawan Jolly and Pedro Estrela},
title = {Development of a Sensitive Multiplexed Open Circuit Potential System for the Detection of Prostate Cancer Biomarkers},
journal = {BioNanoScience},
year = {2017},
volume = {8},
publisher = {Springer Nature},
month = {apr},
url = {https://doi.org/10.1007/s12668-017-0408-0},
number = {2},
pages = {701--706},
doi = {10.1007/s12668-017-0408-0}
}
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Wong, Lai Chun Caleb, et al. “Development of a Sensitive Multiplexed Open Circuit Potential System for the Detection of Prostate Cancer Biomarkers.” BioNanoScience, vol. 8, no. 2, Apr. 2017, pp. 701-706. https://doi.org/10.1007/s12668-017-0408-0.