Anti-anemic Activity of Sodium, Calcium, Iron-Polygalacturonate In Vivo in Rabbits

The aim of this study was to evaluate the antianemic activity of water-soluble PGNaCaFe, a pectin metal complex containing iron and calcium ions for hematopoiesis in vivo. The study of antianemic activity was performed on Chinchilla rabbits of both sexes with an average weight of 2.2–2.5 kg using the Posthemorrhagic Anemia model. The effect of PGNaCaFe was studied after oral administration for 60 days at a dose of 126 mg/kg (with an iron content of 1.3 mg/kg). As a comparison, the well-known antianemic drug-analogue Tot’hema at a dose of 0.25 ml/kg (with an iron content of 1.3 mg/kg) was performed for the correction of the iron deficiency state of rabbits subjected to bloodletting. The results of hematological study of the PGNaCaFe antianemic activity compared to Tot’hema at the end of the experiment show an increase and normalization of blood parameters: the number of erythrocytes increased by 32,1 and 24,3%, hemoglobin concentration—by 38.4 and 40.2%, hematocrit—by 32.1 and 34.5%, respectively. The introduction of iron-containing preparations causes a decrease in the total iron-binding capacity of serum on days 40, 50, and 60 in the first experimental group of rabbits by 21.3, 2.3, and 3.2%, in the second group—by 24.2%, 18.7, and 3.8%, respectively. No pronounced side effects on the internal organs of rabbits were revealed during pathomorphological studies, what demonstrates the advantages of PGNaCaFe which confirms its prospects as non-toxic bioavailable and effective anti-anemic drug.