Journal of the American Society for Mass Spectrometry, volume 29, issue 5, pages 842-852
EThcD Discrimination of Isomeric Leucine/Isoleucine Residues in Sequencing of the Intact Skin Frog Peptides with Intramolecular Disulfide Bond
2
Faculty of Health Sciences, University of Ljubljana, Ljubljana, Slovenia
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3
Faculty of Education, University of Ljubljana, Ljubljana, Slovenia
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Publication type: Journal Article
Publication date: 2018-01-03
Quartile SCImago
Q1
Quartile WOS
Q1
Impact factor: 3.2
ISSN: 10440305, 18791123
Spectroscopy
Structural Biology
Abstract
Our scientific interests involve de novo sequencing of non-tryptic natural amphibian skin peptides including those with intramolecular S-S bond by means of exclusively mass spectrometry. Reliable discrimination of the isomeric leucine/isoleucine residues during peptide sequencing by means of mass spectrometry represents a bottleneck in the workflow for complete automation of the primary structure elucidation of these compounds. MS3 is capable of solving the problem. Earlier we demonstrated the advanced efficiency of ETD-HCD method to discriminate Leu/Ile in individual peptides by consecutive application of ETD to the polyprotonated peptides followed by HCD applied to the manually selected primary z-ions with the targeted isomeric residues at their N-termini and registration of the characteristic w-ions. Later this approach was extended to deal with several (4-7) broad band mass ranges, without special isolation of the primary z-ions. The present paper demonstrates an advanced version of this method when EThcD is applied in the whole mass range to a complex mixture of natural non-tryptic peptides without their separation and intermediate isolation of the targeted z-ions. The proposed EThcD method showed over 81% efficiency for the large natural peptides with intact disulfide ring, while the interfering process of radical site migration is suppressed. Due to higher speed and sensitivity, the proposed EThcD approach facilitates the analytical procedure and allows for the automation of the entire experiment and data processing. Moreover, in some cases it gives a chance to establish the nature of the residues in the intact intramolecular disulfide loops. Graphical Abstract ᅟ.
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Samgina T. Yu. et al. EThcD Discrimination of Isomeric Leucine/Isoleucine Residues in Sequencing of the Intact Skin Frog Peptides with Intramolecular Disulfide Bond // Journal of the American Society for Mass Spectrometry. 2018. Vol. 29. No. 5. pp. 842-852.
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Samgina T. Yu., Kovalev S., Tolpina M. D., Trebše P., Torkar G., Lebedev A. T. EThcD Discrimination of Isomeric Leucine/Isoleucine Residues in Sequencing of the Intact Skin Frog Peptides with Intramolecular Disulfide Bond // Journal of the American Society for Mass Spectrometry. 2018. Vol. 29. No. 5. pp. 842-852.
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TY - JOUR
DO - 10.1007/s13361-017-1857-y
UR - https://doi.org/10.1007%2Fs13361-017-1857-y
TI - EThcD Discrimination of Isomeric Leucine/Isoleucine Residues in Sequencing of the Intact Skin Frog Peptides with Intramolecular Disulfide Bond
T2 - Journal of the American Society for Mass Spectrometry
AU - Samgina, Tatiana Yu
AU - Tolpina, Miriam D
AU - Trebše, Polonca
AU - Kovalev, Sergey
AU - Torkar, Gregor
AU - Lebedev, Albert T.
PY - 2018
DA - 2018/01/03 00:00:00
PB - American Chemical Society (ACS)
SP - 842-852
IS - 5
VL - 29
SN - 1044-0305
SN - 1879-1123
ER -
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@article{2018_Samgina,
author = {Tatiana Yu Samgina and Miriam D Tolpina and Polonca Trebše and Sergey Kovalev and Gregor Torkar and Albert T. Lebedev},
title = {EThcD Discrimination of Isomeric Leucine/Isoleucine Residues in Sequencing of the Intact Skin Frog Peptides with Intramolecular Disulfide Bond},
journal = {Journal of the American Society for Mass Spectrometry},
year = {2018},
volume = {29},
publisher = {American Chemical Society (ACS)},
month = {jan},
url = {https://doi.org/10.1007%2Fs13361-017-1857-y},
number = {5},
pages = {842--852},
doi = {10.1007/s13361-017-1857-y}
}
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MLA
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Samgina, Tatiana Yu., et al. “EThcD Discrimination of Isomeric Leucine/Isoleucine Residues in Sequencing of the Intact Skin Frog Peptides with Intramolecular Disulfide Bond.” Journal of the American Society for Mass Spectrometry, vol. 29, no. 5, Jan. 2018, pp. 842-852. https://doi.org/10.1007%2Fs13361-017-1857-y.
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