Springer Nature
Blood Research
,  volume 59 ,  issue 1 ,  publication number 43

Bone marrow mesenchymal stem cell exosomes suppress JAK/STAT signaling pathway in acute myeloid leukemia in vitro

Publication type: Journal Article
Publication date: 2024-12-20
Springer Nature
scimago Q2
wos Q2
SJR: 0.650
CiteScore: 4.1
Impact factor: 2.8
ISSN: 2287979X, 22880011
Abstract
Introduction

Despite advances in the treatment of acute myeloid leukemia (AML), refractory forms of this malignancy and relapse remain common. Therefore, development of novel, synergistic targeted therapies are needed urgently. Recently, mesenchymal stem cells (MSCs) have been shown to be effective in treating various diseases, with most of their therapeutic outcomes attributed to their exosomes. In the current study, we investigated the effects of bone marrow mesenchymal stem cell (BM-MSC) exosomes on the expression of the Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling genes involved in AML pathogenesis.

Material and Methods

Exosomes were isolated from BM-MSCs and confirmed using transmission electron microscopy, dynamic light scattering, and flow cytometry. Subsequently, the exosome concentration was estimated using the bicinchoninic acid assay, and HL-60 cells were cocultured with 100 µg/mL of BM-MSC exosomes. Finally, the JAK2, STAT3, and STAT5 expression levels were analyzed using qRT-PCR.

Results

The exosome characterization results confirmed that most isolated nanoparticles exhibited a round morphology, expressed CD9, CD63, and CD81, which are specific protein markers for exosome identification, and ranged between 80 and 100 nm in diameter. Furthermore, qRT-PCR analysis revealed a significant downregulation of JAK2, STAT3, and STAT5 in HL-60 cells treated with 100 μg/mL of BM-MSC exosomes.

Conclusion

Since JAK/STAT signaling contributes to AML survival, our findings suggest that the downregulation of JAK/STAT genes by BM-MSC exosomes in leukemic cells may aid in designing a potent therapeutic strategy for AML treatment.

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GOST Copy
Jalilivand S. et al. Bone marrow mesenchymal stem cell exosomes suppress JAK/STAT signaling pathway in acute myeloid leukemia in vitro // Blood Research. 2024. Vol. 59. No. 1. 43
GOST all authors (up to 50) Copy
Jalilivand S., Nabigol M., Bakhtiyaridovvombaygi M., GHAREHBAGHIAN A. Bone marrow mesenchymal stem cell exosomes suppress JAK/STAT signaling pathway in acute myeloid leukemia in vitro // Blood Research. 2024. Vol. 59. No. 1. 43
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Cite this
RIS Copy
TY - JOUR
DO - 10.1007/s44313-024-00051-5
UR - https://link.springer.com/10.1007/s44313-024-00051-5
TI - Bone marrow mesenchymal stem cell exosomes suppress JAK/STAT signaling pathway in acute myeloid leukemia in vitro
T2 - Blood Research
AU - Jalilivand, Sahar
AU - Nabigol, Maryam
AU - Bakhtiyaridovvombaygi, Mehdi
AU - GHAREHBAGHIAN, Ahmad
PY - 2024
DA - 2024/12/20
PB - Springer Nature
IS - 1
VL - 59
PMID - 39704857
SN - 2287-979X
SN - 2288-0011
ER -
BibTex
Cite this
BibTex (up to 50 authors) Copy
@article{2024_Jalilivand,
author = {Sahar Jalilivand and Maryam Nabigol and Mehdi Bakhtiyaridovvombaygi and Ahmad GHAREHBAGHIAN},
title = {Bone marrow mesenchymal stem cell exosomes suppress JAK/STAT signaling pathway in acute myeloid leukemia in vitro},
journal = {Blood Research},
year = {2024},
volume = {59},
publisher = {Springer Nature},
month = {dec},
url = {https://link.springer.com/10.1007/s44313-024-00051-5},
number = {1},
pages = {43},
doi = {10.1007/s44313-024-00051-5}
}